Simultaneous determination of five β-lactam antibiotics (cefepim, ceftazidim, cefuroxim, meropenem and piperacillin) in human plasma by high-performance liquid chromatography with ultraviolet detection

Denooz, Raphaël; Charlier, Corinne

doi:10.1016/j.jchromb.2008.01.037

Cited by 117 publications

(132 citation statements)

References 16 publications

(23 reference statements)

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“…For a comprehensive review of advances in the determination of -lactam antibiotics by liquid chromatography using different detector systems, the reader is referred to the paper by Lara et al [44]. In general, a number of HPLC methods with ultraviolet detection are described for -lactams including simultaneous determination of several -lactams [45][46][47][48][49].…”

Section: Page 8 Of 48mentioning

confidence: 99%

Simultaneous determination of seven β-lactam antibiotics in human plasma for therapeutic drug monitoring and pharmacokinetic studies

Sime

Roberts

et al. 2014

Journal of Chromatography B

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractThere is strong evidence in literature supporting the benefit of monitoring plasma concentrations of -lactam antibiotics in the critically ill to ensure appropriateness of dosing.The objective of this work was to develop a method for the simultaneous determination of total concentrations piperacillin, benzylpenicillin, flucloxacillin, meropenem, ertapenem, cephazolin and ceftazidime in human plasma. Sample preparation involved protein precipitation with acetonitrile containing 0.1% formic acid and subsequent dilution of supernatant with 0.1% formic acid in water. Chromatographic separation was achieved on a reversed phase column (C18, 2.6µm, 2.1* 50 mm) via gradient elution using water and acetonitrile, each containing 0.1% formic acid, as mobile phase. Tandem mass spectrometry (MSMS) analysis was performed, after electrospray ionization in the positive mode, with multiple reaction monitoring (MRM). The method is accurate with the inter-day and intra-day accuracies of quality control samples (QCs) ranging from 95%-107% and 95%-108%, respectively. It is also precise with intra-day and inter-day coefficient of variations ranging from 4 to 12 % and 5-14% respectively. The lower limit of quantification was 0.1g/mL for each antibiotic except flucloxacillin (0.25g/mL). Recovery was greater than 96% for all analytes except for ertapenem (78%). Coefficients of variation for the matrix effect were less than 10% over the six batches of plasma. Analytes were stable over three freeze-thaw cycles, and for reasonable hours on the bench top as well as post-preparation. This novel liquid chromatography tandem mass spectrometry method proved accurate, precise and applicable for therapeutic drug monitoring and pharmacokinetic studies of the selected -lactam antibiotics.Page 5 Highlights  We present a method for simultaneous determination of seven beta-lactams in plasma  The selected antibiotics are those commonly used in critically ill patients  The method is accurate, precise and meets validation requirements by guidelines  It proved applicable for therapeutic drug monitoring and pharmacokinetic studies

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Section: Page 8 Of 48mentioning

confidence: 99%

Simultaneous determination of seven β-lactam antibiotics in human plasma for therapeutic drug monitoring and pharmacokinetic studies

Sime

Roberts

et al. 2014

Journal of Chromatography B

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“…The following methods were devised, based partly on a method describing the extraction of total VAN from serum [24] and the behaviour of CXM chosen as internal standard [25]. For total VAN, thawed samples were subjected …”

Section: Sample Preparation and Hplc Assay Of Free And Total Vancomycinmentioning

confidence: 99%

Correlation between free and total vancomycin serum concentrations in patients treated for Gram-positive infections

Berthoin

Ampe

Tulkens

et al. 2009

International Journal of Antimicrobial Agents

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“…There is a constant requirement for accurate, sensitive and selective analytical methods for monitoring nafcillin antibiotic residues to guarantee the safety of food. Usually, the complexity of food matrices and contaminants presented in food at low concentration (ng g -1 to ȝg g -1 ) levels require analysis performance only after some clean-up and preconcentration steps [2][3][4] , and thus there is a considerable interest in developing new specific and selective methods for extracting and isolating components from complex food matrices. For this purpose, the solid phase extraction (SPE) technique is the most widely used for sample pre-treatment method due to its advantages such as convenience, low cost, timing saving and simplicity.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Application of Core-Shell Structural Carbon Nanotubes-Molecularly Imprinted Composite Material for Determination of Nafcillin in Egg Samples

LIU

Jian

et al. 2013

Chinese Journal of Analytical Chemistry

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Simultaneous determination of five β-lactam antibiotics (cefepim, ceftazidim, cefuroxim, meropenem and piperacillin) in human plasma by high-performance liquid chromatography with ultraviolet detection

Cited by 117 publications

References 16 publications

Simultaneous determination of seven β-lactam antibiotics in human plasma for therapeutic drug monitoring and pharmacokinetic studies

Simultaneous determination of seven β-lactam antibiotics in human plasma for therapeutic drug monitoring and pharmacokinetic studies

Correlation between free and total vancomycin serum concentrations in patients treated for Gram-positive infections

Preparation and Application of Core-Shell Structural Carbon Nanotubes-Molecularly Imprinted Composite Material for Determination of Nafcillin in Egg Samples

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