Simultaneous determination of five coumarins in Angelicae dahuricae Radix by HPLC/UV and LC‐ESI‐MS/MS

Abstract

Rapid, simple and reliable HPLC/UV and LC-ESI-MS/MS methods for the simultaneous determination of five active coumarins of Angelicae dahuricae Radix, byakangelicol (1), oxypeucedanin (2), imperatorin (3), phellopterin (4) and isoimperatorin (5) were developed and validated. The separation condition for HPLC/UV was optimized using a Develosil RPAQUEOUS C(30) column using 70% acetonitrile in water as the mobile phase. This HPLC/UV method was successful for providing the baseline separation of the five coumarins with no interfering peaks detected in the 70% ethanol extract of Angelicae dahuricae Radix. The specific determination of the five coumarins was also accomplished by a triple quadrupole tandem mass spectrometer equipped with an electrospray ionization source (LC-ESI-MS/MS). Multiple reaction monitoring (MRM) in the positive mode was used to enhance the selectivity of detection. The LC-ESI-MS/MS methods were successfully applied for the determination of the five major coumarins in Angelicae dahuricae Radix. These HPLC/UV and LC-ESI-MS/MS methods were validated in terms of recovery, linearity, accuracy and precision (intra- and inter-day validation). Taken together, the shorter analysis time involved makes these HPLC/UV and LC-ESI-MS/MS methods valuable for the commercial quality control of Angelicae dahuricae Radix extracts and its pharmaceutical preparations.

Rapid discrimination of different Apiaceae species based on HPTLC fingerprints and targeted flavonoids determination using multivariate image analysis

“…Several methods have been developed using various chromatographic and spectroscopic tools, mostly high‐performance liquid chromatography (HPLC) and HPLC‐mass spectrometry (MS), for the identification of the botanical origin and/or major constituents in Apiaceae medicinal plants . However, these methods are not efficient for the analysis of large numbers of samples because of the time‐consuming sample preparation steps involved, such as extraction and solvent evaporation.…”

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

“…Park et al reported on LC-MS analysis for five furocoumarins in A. dahuricae [196]. Byakangelicol, oxypeucedanin, imperatorin, phellopterin and isoimperatorin were determined within 5.5 min with gabapentin as the internal standard using QqQ mass spectrometer in MRM mode.…”

Tentative identification of new metabolites of cnidilin by liquid chromatography–mass spectrometry

“…Cnidilin (9-methoxy-4-((3-methylbut-2-en-1-yl)oxy)-7H-furo [3,2-g]chromen-7-one), one of coumarin compounds, represents a major active constituent of Radix Angelicae dahuricae [2,3]. However, there were only a few related studies in vitro research, which contained simultaneous determination of coumarins in Qingxuan Tablets [4], quantitative determination of Radix Angelicae dahuricae (Liquid Chromatography [5], High Liquid Chromatography Mass Spectrometry [5,6], Capillary Electrophoresis [7]), identification of the component [8].…”