Simultaneous Determination of Estradiol and Medroxyprogesterone Acetate in Pharmaceutical Formulations by Second-Derivative Spectrophotometry

Abstract

This paper reports a simple and fast method for the simultaneous determination of estradiol (ED) and medroxyprogesterone acetate (MP) in pharmaceutical formulations by second-derivative spectrophotometry. Methanol was used to extract the drugs from formulations, and subsequently the extracts were evaluated directly by derivative spectrophotometry. The drugs were determined simultaneously by using the graphic method at 297.4 nm for ED and the zero-crossing method at 273.4 nm for MP. If both compounds are present together in a sample, it is possible to quantitate one in the presence of the other. The best signal-to-noise ratio was found when the second derivative of the spectrum was used. The linear ranges for determination of the drugs were 4.7 × 10−6 to 1.6 × 10−4 and 7.2 × 10−6 to 2.0 × 10−4 mol/L for ED and MP, respectively. The ingredients commonly found in commercial pharmaceutical formulations do not interfere with the determination. Chemical and spectral variables were optimized for the determination of both analytes. Good levels of repeatability (relative standard deviation), 1.4 and 1.9%, were obtained for ED and MP, respectively. The proposed method was applied to the determination of these drugs in pharmaceutical formulations.

Simultaneous Determination of Binary Mixture of Estradiol and Progesterone Using Different Spectrophotometric Methods

“…In recent years, analytical methods ultraviolet spectrophotometric method 6,11 , derivative spectrophotometry DS [12][13][14] , HPLC 12,15 , IGC/MS stands for isotope dilution-gas chromatography/mass spectrometry 16 , LCTMS 17 , and GC-MS/MS 4,18 for estradiol and progesterone levels in human serum or plasma samples, as well as a pharmaceutical formulation, have been created. This study aims to develop new and very simple techniques for resolving overlap spectra in their mixtures and pharmaceutical formulations without the need for separation.…”

Simultaneous TLC–densitometric determination of tamoxifen citrate and medroxyprogesterone acetate and UV‐degradation kinetic study of medroxyprogesterone acetate

“…First for TMC there are HPLC (Kikuta & Schmid, 1989;Sanders, Burka, Shelby, Newbold, & Cunningham, 1997), thin-layer chromatography (TLC) (Adam, Gay, & Mood, 1980;Ahmad & Sharifi, 2009;Merey, Galal, Salem, & Abdel-Moety, 2013) spectrophotometry (Boyar & Severcan, 1997;Gamberini, Baraldi, Tinti, Palazzoli, & Ferioli, 2007), spectrofluorimetry (Paterson, Lim, & Smith, 2003;Sarzehi & Chamani, 2010), electrochemical (Aysu & Scheller, 2014;Parandis, Parviz, Reza, & Ali, 2009;Guo, Song, Tian and Song, 2008) and electrophoresis methods (Efstatiade & Aboul-Enein, 1998;Flores, Nevado, Salcedo, & Diaz, 2005;Peris-Vicente, Carda-Broch, & Esteve-Romero, 2014;Flores, Nevado, Berzas, Salcedo Contento, & Cabello, 2004). For MPA, several methods including spectrophotometry (De Beer et al, 2004;Toral, Soto, Richter, & Tapai, 2002), spectrofluorimetry (Ma, Yao, Luo, Pan, & Xu, 2016), HPLC (Dikkeschei, Van Veelen, Nagel, Willemse, & Wolthers, 1985;Rambaud, Bichon, Cesbron, André, & Le Bizec, 2011) and immunoassay (Qi, Wang, Yang, & Liu, 2005;Na et al, 2014;Xu et al, 2005) have been published for determination in pharmaceutical preparations and biological fluids. Although these drugs are commonly coadministered, there is no analytical method for their simultaneous determination.…”

Determinação do 17beta-estradiol nas formas farmacêuticas gel e adesivo transdérmico

“…Na literatura estão descritos alguns métodos alternativos como o uso da voltametria (Salci, Biryol, 2002), cromatografia em camada delgada de alta eficiência (Kotiyan, Vavia, 2000), espectrofotometria derivada de segunda ordem (Toral, Soro, Richter, 2002), entre outros.…”