Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurised liquid extraction and high-performance liquid chromatography

Li, Shaoping; Li, Peng; Lai, Chang-Jiang-Sheng; Gong, Yuanxiang; Kan, Koichi; Dong, Tina Tingxia; Tsim, Karl Wah Keung; Wang, Ying

doi:10.1016/j.chroma.2004.02.080

Cited by 112 publications

(78 citation statements)

References 14 publications

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“…), polysaccharides, ergosterol, mannitol, etc. [30][31][32][33] Chen et al (2008) found that adenosine stimulates adrenal steroidogenesis through the adenosine 2A and 2B receptors and further activates the intracellular Janus kinase 2-mitogen activated protein kinase kinase-extracellular regulated protein kinase signaling cascade. 34) Among these active components, to our knowledge only the nucleosides can stimulate steroidogenesis in Leydig cells (unpublished data).…”

Section: Discussionmentioning

confidence: 99%

Regulatory Mechanisms ofCordyceps sinensison Steroidogenesis in MA-10 Mouse Leydig Tumor Cells

Chen¹,

Huang²

2010

Bioscience, Biotechnology, and Biochemistry

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Section: Discussionmentioning

confidence: 99%

Regulatory Mechanisms ofCordyceps sinensison Steroidogenesis in MA-10 Mouse Leydig Tumor Cells

Chen¹,

Huang²

2010

Bioscience, Biotechnology, and Biochemistry

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“…The profile of nucleosides, nucleobases and uniquely modified nucleosides, instead of any single metabolite, can be feasible for the quality control of Cordyceps sensu lato products (Shiao et al 1994; Xiao and Xiong 2013). The nucleoside profile of Ophiocordyceps species and its allies has been explored, and the analytical methods of determining nucleosides and nucleobases in Cordyceps have been reported previously (Li et al 2004; Fan et al 2006; Yang et al 2007, 2009; Xiao and Xiong 2013; Zhang et al 2014; Qian and Li 2017). High performance liquid chromatography (HPLC), combined with ultraviolet (UV), evaporative light scattering detector or mass spectra, has been applied to analyse the profile of nucleosides, nucleobases in Cordyceps -related species, and proved to be a simple, convenient and reliable method.…”

Section: Introductionmentioning

confidence: 99%

Determination and comparative analysis of 13 nucleosides and nucleobases in natural fruiting body of Ophiocordyceps sinensis and its substitutes

et al. 2017

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Nucleosides and nucleobases are one of the most important indicators of quality control. A sensitive and reliable high performance liquid chromatography-ultraviolet method was applied to analyse 13 nucleosides and nucleobases simultaneously in 15 batches of nine Ophiocordyceps species and its allies in China. Principal component analysis (PCA) and cluster analysis were conducted by SPSS 22.0 software (IBM Corp., Armonk, NY, USA). The 15 samples of Cordyceps were differentiated successfully based on their nucleoside and nucleobase content. Total nucleosides content in mycelium was significantly higher than that in the natural fruiting bodies of Ophiocordyceps sinensis (NFOS). Five nucleosides or nucleobases – adenine (A), guanosine (Gu), uracil (U), uridine (Ur) and guanine (G) – were the major components contributed to the total variance according to PCA. The profiles of the 13 tested nucleosides and nucleobases (including adenosine, cytidine, guanosine, inosine, thymidine, uridine, cordycepin, adenine, cytosine, guanine, thymine, uracil and hypoxanthine) can discriminate different samples and can be candidate indicators applied for the quality control of Ophiocordyceps and its allies.

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“…Better separation efficiency and sensitivity were achieved with acetonitrile than methanol [21]. Using 0.2% acetic acid and triethylamine in the solvent system can reduce the ionization of alkaloids and give more symmetrical peaks and a better separation [31,32]. The proportion of each mobile phase gradient is closely related to the resolution since all studied alkaloids cannot be well separated by isocratic elution [31].…”

Section: Analysis Of the Samplesmentioning

confidence: 99%

“…Using 0.2% acetic acid and triethylamine in the solvent system can reduce the ionization of alkaloids and give more symmetrical peaks and a better separation [31,32]. The proportion of each mobile phase gradient is closely related to the resolution since all studied alkaloids cannot be well separated by isocratic elution [31]. The changes in the optimized gradient elution conditions can cause incomplete separation and overlapping peaks.…”

Section: Analysis Of the Samplesmentioning

confidence: 99%

Simultaneous determination of eleven alkaloids in Corydalis decumbens by HPLC with diode-array detection

Gu¹,

Yang²,

Wang³

et al. 2017

Acta Chromatographica

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Summary.A high-performance liquid chromatography-diode-array detection method was developed and validated to determine simultaneously eleven major alkaloids in Corydalis decumbens (Thunb.) Pers. The alkaloids detected were corlumidine, protopine, coptisine, tetrahydrojatrorrhizine, palmatine, berberine, sanguinarine, papaverine hydrochloride, tetrahydropalmatine, bicuculline, and corydaline. Chromatographic separation was achieved using a C-18 column with a mobile phase composed of A (0.2% acetic acid solution, adjusted with triethylamine to pH 5.0) and B (acetonitrile), with stepwise gradient elution. Ultraviolet diode-array detection was used; chromatograms were examined at the wavelength of 280 nm. The regression equations showed a good linear relationship between the peak area of each marker and concentration (r = 0.9994-0.9999). The recovery values ranged between 93.66% and 100.54%. The method was fully validated with respect to detection and quantification limits, precision, reproducibility, and accuracy. The described high-performance liquid chromatography (HPLC) method was successfully used for the differentiation and quantification of the eleven major alkaloids in C. decumbens (Thunb.) Pers. and can be considered an effective procedure for the analyses of this important class of natural compounds.

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Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurised liquid extraction and high-performance liquid chromatography

Cited by 112 publications

References 14 publications

Regulatory Mechanisms ofCordyceps sinensison Steroidogenesis in MA-10 Mouse Leydig Tumor Cells

Regulatory Mechanisms ofCordyceps sinensison Steroidogenesis in MA-10 Mouse Leydig Tumor Cells

Determination and comparative analysis of 13 nucleosides and nucleobases in natural fruiting body of Ophiocordyceps sinensis and its substitutes

Simultaneous determination of eleven alkaloids in Corydalis decumbens by HPLC with diode-array detection

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