Simultaneous Determination of Epinephrine, Noradrenaline and Dopamine in Human Serum Samples by High Performance Liquid Chromatography with Chemiluminescence Detection

Abstract

A simple, rapid and accurate high performance liquid chromatographic (HPLC) technique coupled with chemiluminescence (CL) detection was developed for the simultaneous determination of epinephrine (E), noradrenaline (NA) and dopamine (DA). It was based on the analyte enhancement effect on the CL reaction between luminol and potassium ferricyanide. The effects of various parameters, such as potassium ferricyanide concentration, luminol concentration, pH value and component of the mobile phase on chromatographic behaviors of the analytes (E, NA and DA) were investigated. The separation was carried out on C 18 column using the mobile phase of 0.01 mol/L potassium hydrogen phthalate solution and methanol (92∶8, V/V). Under the optimum conditions, E, NA and DA showed good linear relationships in the range of 1×10 -8 -5×10 -6 , 5.0×10 -9 -1.0×10-6and 5.0×10 -9 -1.0×10 -6 g/mL respectively. The detection limits for E, NA and DA were 4.0×10 -9 , 1.0×10and 8.0×10 -10 g/mL. The proposed method has been applied successfully to the analysis of E, NA and DA in human serum samples.

Enhancement of chemiluminescence of the KIO4–luminol system by gallic acid, acetaldehyde and Mn2+: application for the determination of catecholamines

“…[16][17][18][19] The advantages of CL technique include superb sensitivity, wide dynamic range, simple instrumentation and easy automation. Some of the reported CL methods utilized the enhancing effect of catetcolamines on the CL emission of luminol-periodate, [8,[12][13][14]19] while others took advantage of their inhibiting effect on the CL emission from the oxidation of luminol with hypochlorite, [7] ferricyanide, [9,10,17,18] chlorate, [11] iodine, [15] or hydrogen peroxide. [16] The detection limits of catecholamines for these methods varied from subnmol/L [12,13] to μmol/L.…”

“…However, some of these methods lack sensitivity and selectivity or require derivation steps or sophisticated instruments. Another attractive method for sensitive detection of catechoamines is based on chemiluminescence (CL), which is usually coupled with flow injection (FI), [7][8][9][10][11][12][13] high-performance liquid chromatography (HPLC) [14,15] or capillary electrophoresis (CE). [16][17][18][19] The advantages of CL technique include superb sensitivity, wide dynamic range, simple instrumentation and easy automation.…”

Indirect Chemiluminescence-based Determination of Catecholamines in Pharmaceutical Formulations by Furandicarboxylate Derivative as a Novel Blue Fluorescer in Peroxyoxalate-H2O2 System

“…However, some of these methods lack sensitivity and selectivity or require derivation steps or sophisticated instruments. Another attractive method for sensitive detection of CAs is based on chemiluminescence (CL), which is usually coupled with flow injection (FI), [12][13][14][15][16][17][18][19] high-performance liquid chromatography (HPLC) 20,21 or capillary electrophoresis (CE). [22][23][24][25] The advantages of the CL technique include superb sensitivity, wide dynamic range, simple instrumentation and easy automation.…”

Highly selective detection of Epinephrine at oxidized Single-Wall Carbon Nanohorns modified Screen Printed Electrodes (SPEs)

“…A number of methods were reported for the determination of catecholamines by spectrophotometry (Salem, 1987), capillary electrophoresis (Peterson et al, 2002) and chromatography equipped with an electrochemical detection (Patel and Chau, 2008), chemiluminescence (Chen et al, 2007) and fluorescence (Fotopoulou and Ioannou, 2002). Among them high-performance liquid chromatography (HPLC) coupled to electrochemical detection offered advantages of high resolution with low detection limits (Holmes et al, 1994).…”