Simultaneous determination of eight ginsenosides in Zibu Piyin Recipe using ultra-performance liquid chromatography coupled with triple-quadrupole mass spectrometry (UPLC-MS/MS)

Abstract

A simple, sensitive, selective, and reliable ultra-performance liquid chromatograph-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the simultaneous detection and quantification of eight compounds (ginsenosides Rg 1 , Re, Rf, Rb 3 , Ro, Rd, and 20(S)-and 20(R)-ginsenoside-Rg 2 ) found in Zibu Piyin Recipe (ZBPYR). These compounds were separated within 20 min on a ZORBAX RRHD Eclipse Plus C18 column with acetonitrile and 0.1% formic acid as the mobile phase. The column was coupled to an ESI-triplequadrupole mass spectrometer operated in positive ion mode. The eight ginsenosides were quantified in multiple reaction monitoring acquisition mode. Regression analyses revealed good linear relationships (R ¼ 0.9986-0.9993) within the test ranges examined. Variations in the inter-and intra-day analytical precision for all analyses were less than 2.96%. The analytical accuracy, evaluated by a recovery test at three concentrations, was in the range of 96.51-104.94% (relative standard deviation < 4.5%). The proposed method is accurate, sensitive, and reliable. The 8 ginsenoside compounds were also quantified using this method in 10 ZBPYR sample batches, and the results provide a new basis for the overall assessment of ZBPYR quality.

Simultaneous determination of ten constituents in chaiqin qingning capsule by high-performance liquid chromatography coupled with triple-quadrupole mass spectrometry

“…According to the State FDA of China, baicalin was chosen as index component of CQQNC for quality control, but as a compound preparation, multi-index components determination which was used to chemically characterize the similarity and distinctness between the different batches, serving for their quality control, and overall assessment were necessary. [ 3 4 ] Second, two groups compound with similar structure and similar molecular weight were obtained good separation, which including one group of cholic acid, taurocholic acid, deoxycholic acid, hyodeoxycholic acid and chenodeoxycholic acid, another group of saikosaponin A, saikosaponin B1, saikosaponin C, and saikosaponin D. Third, during the specific and sensitive ESI-MS/MS detection, MRM method was used to exclude false-positive finding. Fourth, the values of LOD and LOQ under our method were less than the compounds in other references.…”