Simultaneous Determination of Dopamine, Serotonin and Ascorbic Acid at a Glassy Carbon Electrode Modified with Carbon-Spheres

Abstract

A novel glassy carbon electrode (GCE) modified with carbon-spheres has been fabricated through a simple casting procedure. The modified GCE displays high selectivity and excellent electrochemical catalytic activities towards dopamine (DA), serotonin (5-HT), and ascorbic acid (AA). In the co-existence system, the peak separations between AA and DA, DA and 5-HT, and AA and 5-HT are large up to 230, 180, and 410 mV, respectively. Differential pulse voltammetry (DPV) has been employed to simultaneously detect DA, 5-HT, and AA, and the linear calibration curves for DA, 5-HT, and AA are obtained in the range of 20.0–150.0 μM, 40.0–750.0 μM and 300.0–2,000.0 μM with detection limits (S/N = 3) of 2.0 μM, 0.7 μM and 0.6 μM, respectively. The proposed electrode has been applied to detect DA, 5-HT, and AA in real samples using standard addition method with satisfactory results.

Fused and unzipped carbon nanotubes, electrochemically treated, for selective determination of dopamine and serotonin

“…oxide with Pt nanoparticles [23], or GO-porphyrin electrochemically reduced [17]. There are few reports in literature about simultaneous determination of DA, 5HT and AA [26].…”

Recent Advances in Electroanalysis of Vitamins

“…This because it is not only the most common anti‐oxidant, but is also present in mammalian brain along with several neurotransmitters, so its determination is also crucial in the medical field. Electrochemical methods for its determination were recently reviewed , nevertheless many others were proposed since then . Many of these methods allow the determination of ascorbic acid in the presence of several other analytes, more commonly uric acid and dopamine since they are co‐existing in biological matrixes and they are oxidized at almost the same potential at traditional electrode materials.…”

“…Many of these methods allow the determination of ascorbic acid in the presence of several other analytes, more commonly uric acid and dopamine since they are co‐existing in biological matrixes and they are oxidized at almost the same potential at traditional electrode materials. Other methods were demonstrated successfully for mixture of ascorbic acid with some drugs , especially acetaminophen or components regulating the physiological function of organisms .…”

Sensitive determination of norepinephrine, epinephrine, dopamine and 5‐hydroxytryptamine by coupling HPLC with [Ag(HIO6)2]5−–luminol chemiluminescence detection

“…play a vital function in evaluating the activity of the central nervous systems, explaining the disease mechanism and supporting the diagnosis of many diseases (Westerink and Timmerman, 1999;Hussain and Lokhandwala, 2003). 5-HT makes a significant contribution to brain functions such as sleep, thermoregulation and food intake, as well as psychopathological states such as depression and anxiety (Zhou et al, 2013). For example, the deficiency of DA concentration in brain may lead to Parkinson's disease and schizophrenia (Kienast and Heinz, 2006;Tsunoda et al, 2010).…”

“…For example, the deficiency of DA concentration in brain may lead to Parkinson's disease and schizophrenia (Kienast and Heinz, 2006;Tsunoda et al, 2010). 5-HT makes a significant contribution to brain functions such as sleep, thermoregulation and food intake, as well as psychopathological states such as depression and anxiety (Zhou et al, 2013). Hence, it is important to develop a simple, rapid and sensitive method to determine monoamine neurotransmitters owing to their important roles in clinical diagnosis and medical treatment.…”