Simultaneous determination of domperidone and cinnarizine in a binary mixture using derivative spectrophotometry, partial least squares and principle component regression calibration

Abstract

This work is concerned with the simultaneous determination of domperidone maleate (DOM) and cinnarizine (CINN) in a binary mixture form, without previous separation, by two different techniques. The first method is the application of derivative spectrophotometry where the linearity range and percentage recoveries for DOM and CINN were 2.5-30 micro g mL(-1), 5-25 micro g mL(-1) and 100.06+/-1.157, 99.93+/-1.377, respectively. The second method depends on the application of partial least squares (PLS) and principle component regression (PCR) models. A training set consisting of 10 mixtures containing 5-20 micro g mL(-1) for each component was used for the construction of the PCR and PLS models. These models were used after their validation for the prediction of the concentration of DOM and CINN in their mixtures. The proposed procedures were successfully applied for the simultaneous determination of both drugs in laboratory prepared mixtures and in commercial tablet preparations. The validity of the proposed methods was assessed by applying the standard addition technique where the percentage recovery of the added standard was found to be 99.98+/-0.297 and 99.84+/-0.700 for DOM and CINN, respectively, using the derivative spectrophotometric method and 100.29+/-0.398 and 100.11+/-0.363 for DOM and CINN, respectively, using the PLS and PCR methods. The proposed procedures are rapid, simple, require no preliminary separation steps and can be used for routine analysis of both drugs in quality control laboratories.

Simultaneous determination of Fe(III) and Al(III) by first-derivative spectrophotometry and partial least-squares (PLS-2) method – Application to post-haemodialysis fluids

“…In the literature it is described that a sufficiently high maximum number of factors must be assayed to calculate the optimum number of factors, using PRESS value analysis. In our case a number of 8 was selected as convenient because the number of standards employed was 14 (half the number of total standards +1 is recommended in the literature [33]). The root mean squares error (RMSE), which is an indication of the average error in the analysis, for each component, was determined for the calibration (RMSEC) and prediction (RMSEP) sets, according to…”

“…The combination of derivative techniques with multivariate calibration methods has been proposed [23][24][25][26][27][28][29][30][31][32][33]. PLS methods present two modifications known as PLS-1 and PLS-2.…”

Development of a method for the optical in-situ determination of pH value during high-pressure treatment of fluid food

“…The use of multivariate data modeling for spectroscopic data has already been proved at ambient pressure (e.g. Heise & Bittner, 1997;Salem, El-Zanfaly, El-Tarras, & El-Bardicy, 2003;Seemann, Rapp, Zell, & Gauglitz, 1997). Fig.…”

Sensitive and Selective Extraction Free Ion-Pair Complexometric Determination of Domperidone in Pharmaceuticals and in Spiked Human Urine

“…The most widely used technique for the assay of DOM has been UV spectrophotometry. Several UV-spectrophotometric [24][25][26][27][28][29][30][31][32][33][34][35][36] procedures employing different media have been reported for assay in single as well as in combined dosage forms. There is only one report on the visible spectrophotometric assay of DOM in pharmaceuticals 37 in which four procedures are described.…”