Simultaneous Determination of Different Classes of Lipid Hydroperoxides by High-Performance Liquid Chromatography with Post Column Detection by a Ferrous/Xylenol Orange Reagent

Sugino, Keiji

doi:10.1271/bbb.63.773

Cited by 15 publications

(3 citation statements)

References 18 publications

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“…Moreover, an HPLC-fluorescence method using postcolumn detection was effectively applied in the analyses of hydroperoxide mixtures containing conjugated and nonconjugated diene structures [ 71 ]. The simultaneous determination of different classes of lipid hydroperoxides by HPLC with post-column detection by a ferrous xylenol orange reagent (FOX) was also reported [ 72 ].…”

Section: Chromatographic Methodsmentioning

confidence: 99%

Analytical and Structural Tools of Lipid Hydroperoxides: Present State and Future Perspectives

Kontogianni

Gerothanassis

2022

Molecules

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Mono- and polyunsaturated lipids are particularly susceptible to peroxidation, which results in the formation of lipid hydroperoxides (LOOHs) as primary nonradical-reaction products. LOOHs may undergo degradation to various products that have been implicated in vital biological reactions, and thus in the pathogenesis of various diseases. The structure elucidation and qualitative and quantitative analysis of lipid hydroperoxides are therefore of great importance. The objectives of the present review are to provide a critical analysis of various methods that have been widely applied, and more specifically on volumetric methods, applications of UV-visible, infrared, Raman/surface-enhanced Raman, fluorescence and chemiluminescence spectroscopies, chromatographic methods, hyphenated MS techniques, NMR and chromatographic methods, NMR spectroscopy in mixture analysis, structural investigations based on quantum chemical calculations of NMR parameters, applications in living cells, and metabolomics. Emphasis will be given to analytical and structural methods that can contribute significantly to the molecular basis of the chemical process involved in the formation of lipid hydroperoxides without the need for the isolation of the individual components. Furthermore, future developments in the field will be discussed.

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Section: Chromatographic Methodsmentioning

confidence: 99%

Analytical and Structural Tools of Lipid Hydroperoxides: Present State and Future Perspectives

Kontogianni

Gerothanassis

2022

Molecules

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“…This method is highly sensitive and pretty versatile considering both column adapted to HPLC separation [25]. Specific hydroperoxides generated from sterols can also be assessed by liquid chromatography.…”

Section: Liquid Chromatographymentioning

confidence: 99%

A review of analytical methods measuring lipid oxidation status in foods: a challenging task

Barriuso

Astiasarán

Ansorena

2012

Eur Food Res Technol

267

220

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Lipid oxidation analysis in food samples is a relevant topic since compounds generated in the process are related to undesirable sensory and biological effects. As the process is complex and depends on the type of lipid substrate, oxidation agents and environmental factors, proper measurement of lipid oxidation remains a challenging task. A great variety of methodologies have been developed and implemented so far, both for determining primary oxidation products and secondary oxidation products. Most common methods and classical procedures are described, including peroxide value, TBARS analysis and chromatography. Some other methodologies such as chemiluminescence, fluorescence emission, Raman spectroscopy, infrared spectroscopy or magnetic resonance, provide interesting and promising results, so attention must be paid to these alternative techniques in the area of food lipid oxidation analysis.

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“…Over the past few years, numerous new methods have been developed for measuring PV. These studies have described rapid methods for the quantitative determination of PV of vegetable oils and different foods by FTIR transmission spectroscopy (3)(4)(5); by coordination ion spray mass spectrometry (CIS/MS) (6); by GC-MS (7); by HPLC (8)(9)(10)(11)(12)(13)(14); by IR and NMR spectroscopic techniques (15); by conventional, difference, or difference-derivative UV spectrophotometry (16); by thermogravimetry (17); by measuring thermoluminescence (18); and by using fluorescence and autofluorescence spectroscopy (19,20). A simple and sensitive spectrophotometric lipid hydroperoxide measurement using oxidation of ferrous (Fe 2+ ) to ferric ions (Fe 3+ ) by hydroperoxides has already been developed.…”

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confidence: 99%

Comparison of four analytical methods for the determination of peroxide value in oxidized soybean oils

Yildiz

Wehling

Cuppett

2003

J Americ Oil Chem Soc

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Previous work in our laboratory demonstrated that soybean oil oxidation, expressed as PV, can be determined using NIR transmission spectroscopy as an alternative to the official AOCS iodometric titration method. In the present study, a comparison of four peroxide analytical methods was conducted using oxidized soybean oil. The methods included the official AOCS iodometric titration, the newly developed NIR method, the PeroxySafe™ kit, and a ferrous xylenol orange (FOX) method, the latter two being colorimetric methods based on oxidation of iron. Five different commercially available soybean oils were exposed to fluorescent light to obtain PV levels of 0-20 meq/kg; periodic sampling was done to ensure having representative samples throughout the designated range. A total of 46 oil samples were analyzed. Statistical analysis of the data showed that the correlation coefficient (r) and standard deviation of differences (SDD) between the standard titration and NIR methods were r = 0.991, SDD = 0.72 meq/kg; between titration and the PeroxySafe™ kit were r = 0.993, SDD = 0.56 meq/kg; and between the standard titration and FOX method were r = 0.975, SDD = 2.3 meq/kg. The high correlations between the titration, NIR, and PeroxySafe™ kit data indicated that these methods were equivalent.

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Simultaneous Determination of Different Classes of Lipid Hydroperoxides by High-Performance Liquid Chromatography with Post Column Detection by a Ferrous/Xylenol Orange Reagent

Cited by 15 publications

References 18 publications

Analytical and Structural Tools of Lipid Hydroperoxides: Present State and Future Perspectives

Analytical and Structural Tools of Lipid Hydroperoxides: Present State and Future Perspectives

A review of analytical methods measuring lipid oxidation status in foods: a challenging task

Comparison of four analytical methods for the determination of peroxide value in oxidized soybean oils

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