Simultaneous determination of dexamethasone and trimethoprim by liquid chromatography

Gallego, Jorge; Arroyo, J. Pérez

doi:10.1016/s0731-7085(02)00468-5

Cited by 24 publications

(12 citation statements)

References 23 publications

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“…Dexamethasone (8S,9R,10S,11S,13S,14S,1 6R,17R)-9-Fluoro-11,17-dihydroxy-17-(2-hydroxyacetyl)-10,13,16-trimethyl-, 7,8,9,10,11,12,13,14,15,16,17-dodecahydro-3H-cyclopenta[a] phenanthren-3-one [ Figure 1] a corticosteroid, is used to relieve bacterial ocular infections that require corticosteroid [1]. Many authors have reported a lot of different analytical methods for both drugs individually utilizing different techniques like HPLC [2][3][4][5][6][7][8][9], spectrophotometry and spectrofluorometric [10][11][12][13], titrimetric [14] methods for ciprofloxacin while for dexamethasone different methods involving HPLC, GC and TLC are reported [15][16][17][18][19][20][21].…”

Section: Ciprofloxacinmentioning

confidence: 99%

Stability Indicating Rp-HPLC Method for Simultaneous Determination of Ciprofloxacin and Dexamethasone in Binary Combination

Razzaq

Ashfaq

Mariam³

et al. 2017

J. Chil. Chem. Soc.

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A simple and isocratic HPLC method with stability indicating nature was developed and then subsequently validated for simultaneous determination of ciprofloxacin and dexamethasone in pharmaceutical formulations, human serum and urine. Best chromatographic separations were attained within run time of 10 minutes using C8 as stationary phase and mixture of phosphate buffer and methanol (41:59 v/v) as mobile phase. The mobile phase was flowed at 1.5 mL min -1 with detection of both the analytes at 270 nm using photodiode array detector. Validation of the method was accomplished using specificity, linearity, accuracy, precision, robustness, LOD and LOQ. The method was found linear from 3-21 mg mL -1 for ciprofloxacin (r 2 ≥ 0.999) and 1-7 mg mL -1 for dexamethasone (r 2 ≥ 0.999). The %age recoveries of ciprofloxacin in spiked human urine and serum were ≥99% and ≥85% respectively, while for dexamethasone they were ≥97% in both matrices. The method proficiently separated the peaks of ciprofloxacin and dexamethasone from all types of interfering substances including degradation products/impurities with purity index ≥ 0.9998. The method thus was stability-indicating and can be employed for simultaneous analysis of ciprofloxacin and dexamethasone in complex matrices involving multiple components in the mixture. 1-INTRODUCTION Ciprofloxacin(1-cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-quinoline-3-carboxylic acid), a well-known antibiotic, is used to relieve bacterial infections of the eyes, corneal ulcers along with some common bacterial attacks. Dexamethasone (8S,9R,10S,11S,13S,14S,1 6R,17R)-9-Fluoro-11,17-dihydroxy-17-(2-hydroxyacetyl)-10, 13,16-trimethyl-,7,8,9,10,11,12,13,14,15,16,17-dodecahydro-3H-cyclopenta[a] phenanthren-3-one [ Figure 1] also the enforcement agencies in general to monitor the quality of the marketed products. 2-EXPERIMENTAL Chemicals and ReagentsCiprofloxacin (99.87%) and dexamethasone (99.96%) reference standards were received from Schazoo Zaka Laboratories (Lahore, Pakistan) and were used as such without further refinement. Commercial products (Zoxan D and Ciprodex) containing 3 mg mL -1 ciprofloxacin and 1 mg mL -1 dexamethasone were analyzed during the current research work. All the chemicals used were of either HPLC grade or highest grade available commercially and were used as such. Double distilled water, other liquids used in mobile phase and all the injecting solutions were subject to filtration employing 0.45 mm nylon filters (Millipore, USA). Equipment and Chromatographic ConditionsAll the chromatographic work was conducted on LC-20A system (Shimadzu, Japan) using PDA detector at 270 nm. Each time 20 mL volume of analytes were injected using the fixed loop and peak areas of ciprofloxacin and dexamethasone were integrated using system built software. For separation, Hypersil BDS C8 column (250 X 4.6 mm, 5 mm) was used at room temperature. Pre-mixed mobile phase was used which was prepared by mixing 59:41 v/v ratio of methanol and 0.018M phosphate buffer (pH 3.0) and was flowe...

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Section: Ciprofloxacinmentioning

confidence: 99%

Stability Indicating Rp-HPLC Method for Simultaneous Determination of Ciprofloxacin and Dexamethasone in Binary Combination

Razzaq

Ashfaq

Mariam³

et al. 2017

J. Chil. Chem. Soc.

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“…The HPLC conditions used were adapted from methods previously reported (Gallego and Arroyo, 2002 concentration of samples collected during the in vitro permeability assay, were generated for TMP (R t = 5.98 ± 0.02 min, y = 140188x + 4622.9, R 2 = 0.9998) and was linear from 0.25 µM to 10 µM. The limit of detection (LOD, signal-to-noise ratio 3-to-1) was 0.1 µM and the limit of quantification (LOQ, signal-tonoise ratio 10-to-1) was 0.25 µM.…”

Section: Tmp High Performance Liquid Chromatography (Hplc) Assaymentioning

confidence: 99%

Antibacterial Activity of β-Cyclodextrin and 2-Hydroxypropyl-β-Cyclodextrin Trimethoprim Complexes

M.¹

2011

American Journal of Microbiology

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“…The volume was completed to 1,000 ml using distilled water according to our previous research (32). DEX was detected at 242 nm with a mobile phase of 0.01 mol/L phosphate buffer (pH 3.0) and acetonitrile (ratio 25:75, v/v) at a flow rate of 1.0 ml/min according to reference (33). The mobile phase for 5-FU consisted of acetonitrile and 0.0364 mol/L monobasic potassium phosphate in distilled water (1:99, v/v), the wavelength was set at 265 nm (34).…”

Section: Drug Analysismentioning

confidence: 99%

In vitro Enhancement of Lactate Esters on the Percutaneous Penetration of Drugs with Different Lipophilicity

et al. 2010

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Abstract. Lactate esters are widely used as food additives, perfume materials, medicine additives, and personal care products. The objective of this work was to investigate the effect of a series of lactate esters as penetration enhancers on the in vitro skin permeation of four drugs with different physicochemical properties, including ibuprofen, salicylic acid, dexamethasone and 5-fluorouracil. The saturated donor solutions of the evaluated drugs in propylene glycol were used in order to keep a constant driving force with maximum thermodynamic activity. The permeability coefficient (K p ), skin concentration of drugs (SC), and lag time (T), as well as the enhancement ratios for K p and SC were recorded. All results indicated that lactate esters can exert a significant influence on the transdermal delivery of the model drugs and there is a structure-activity relationship between the tested lactate esters and their enhancement effects. The results also suggested that the lactate esters with the chain length of fatty alcohol moieties of 10-12 are more effective enhancers. Furthermore, the enhancement effect of lactate esters increases with a decrease of the drug lipophilicity, which suggests that they may be more efficient at enhancing the penetration of hydrophilic drugs than lipophilic drugs. The influence of the concentration of lactate esters was evaluated and the optimal concentration is in the range of 5∼10 wt.%. In sum, lactate esters as a penetration enhancer for some drugs are of interest for transdermal administration when the safety of penetration enhancers is a prime consideration.

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Simultaneous determination of dexamethasone and trimethoprim by liquid chromatography

Cited by 24 publications

References 23 publications

Stability Indicating Rp-HPLC Method for Simultaneous Determination of Ciprofloxacin and Dexamethasone in Binary Combination

Stability Indicating Rp-HPLC Method for Simultaneous Determination of Ciprofloxacin and Dexamethasone in Binary Combination

Antibacterial Activity of β-Cyclodextrin and 2-Hydroxypropyl-β-Cyclodextrin Trimethoprim Complexes

In vitro Enhancement of Lactate Esters on the Percutaneous Penetration of Drugs with Different Lipophilicity

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Simultaneous determination of dexamethasone and trimethoprim by liquid chromatography

Cited by 24 publications

References 23 publications

Stability Indicating Rp-HPLC Method for Simultaneous Determination of Ciprofloxacin and Dexamethasone in Binary Combination

Stability Indicating Rp-HPLC Method for Simultaneous Determination of Ciprofloxacin and Dexamethasone in Binary Combination

Antibacterial Activity of &beta;-Cyclodextrin and 2-Hydroxypropyl-&beta;-Cyclodextrin Trimethoprim Complexes

In vitro Enhancement of Lactate Esters on the Percutaneous Penetration of Drugs with Different Lipophilicity

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Antibacterial Activity of β-Cyclodextrin and 2-Hydroxypropyl-β-Cyclodextrin Trimethoprim Complexes