Simultaneous determination of dehydroepiandrosterone, its 7-hydroxylated metabolites, and their sulfates in rat brain tissues

Kazihnitková, Hana; Tejkalová, H.; Benešová, O; Bičı́ková, Marie; Hill, Martin; Hampl, Richard

doi:10.1016/j.steroids.2004.06.002

Cited by 17 publications

(12 citation statements)

References 19 publications

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“…By contrast, in the old rats the maximum stimulatory effect was seen at the higher (1.0 mg) dose of DHEA (Tables 2, 3, 4, 5, 6 and 7). This is consistent with the reported low levels of DHEA (10% of adult value) in the old population (Hinson and Raven 1999;Parker 1999) and in rats (Kazihnitkova et al 2004;Ren and Hou 2005;Vallee et al 2000;Weill-Engerer et al 2003). Of interest to note in this context is our earlier observation that high dose of 2.0 mg had adverse affects on respiratory activities of the liver as well as the brain mitochondria (Patel and Katyare 2007).…”

Section: Discussionsupporting

confidence: 88%

“…In general, the higher dose of DHEA seemed to have a greater stimulatory effect in the old rats (Tables 2, 3, 4, 5, 6 and 7). This may perhaps relate to the declining levels of DHEA in the old animals (Kazihnitkova et al 2004;Ren and Hou 2005;Vallee et al 2000;Weill-Engerer et al 2003). From the data presented one may also tend to think that with respect to respiratory activities the effect was more pronounced in the young animals than in the old animals.…”

Section: Discussionmentioning

confidence: 82%

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Stimulation of oxidative energy metabolism in liver mitochondria from old and young rats by treatment with dehydroepiandrosterone (DHEA). A comparative study

Patel

Modi

Katyare

2007

AGE

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Effects of treatment with DHEA (0.2 or 1.0 mg/kg body weight for 7 days) on oxidative energy metabolism of rat liver mitochondria from old (18-24 month old) and young (8-10 weeks old) male albino rats belonging to Charles-Foster strain were examined. Treatment with 1.0 mg DHEA resulted in increased body weights of the young rats without change in the liver weight. In the old animals the liver weight increased progressively with increasing dose of DHEA without affecting body weight. The state 3 respiration rates in liver mitochondria from old animals were, in general, lower than those in the young rats. The state 3 and state 4 respiration rates increased following DHEA treatment in dose-dependent manner bringing them close to values for young animals or beyond that with the effect being more pronounced at 1.0 mg dose. Treatment with DHEA also stimulated state 3 and state 4 respiration rates in young rats in dose-dependent manner. Contents of cytochrome aa(3), b and c + c(1) increased significantly in old animals in dose-dependent manner. In the young rats the lower dose (0.2 mg) of DHEA was more effective in bringing about a maximum increase in the contents of the cytochromes; the effect declined at the higher dose (1.0 mg). DHEA treatment also stimulated the mitochondrial ATPase activity in the old as well as in the young rats. The dehydrogenases activities were considerably low in the old rats compared to the values for the young animals. Treatment with DHEA stimulated dehydrogenases activities in old rats in dose-dependent manner bringing them close to values for the young animals or beyond. Treatment with lower dose (0.2 mg) of DHEA maximally stimulated dehydrogenases activities in young animals.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 82%

Stimulation of oxidative energy metabolism in liver mitochondria from old and young rats by treatment with dehydroepiandrosterone (DHEA). A comparative study

Patel

Modi

Katyare

2007

AGE

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“…It has been shown that the concentration of DHEA, a neurosteroid ''antiaging'' hormone, declines with aging in the brain (Kazihnitková et al 2004). In the present study, in vivo DHEA administration was tested for its tentative neuroprotection and antioxidative role.…”

Section: Discussionmentioning

confidence: 99%

Effect of dehydroepiandrosterone (DHEA) on monoamine oxidase activity, lipid peroxidation and lipofuscin accumulation in aging rat brain regions

et al. 2008

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Dehydroepiandrosterone (DHEA), one of the major steroid hormones, and its ester have recently received attention with regard to aging and age-related diseases like Alzheimer and others. DHEA is synthesized de novo in the brain and its substantial fall with age has been shown to be associated with neuronal vulnerability to neurotoxicity processes. Thus, DHEA is considered to be a neuroactive pharmacological substance with potential antiaging properties. A prominent feature that accompanies aging is an increase in monoamine oxidase (MAO). Increased MAO activity with correlated increase in lipid peroxidation in the aging rat brain supports the hypothesis that catecholamine oxidation is an important source of oxidative stress. The progressive accumulation of lipofuscin in neuronal cells is one of the most characteristic age related changes, an increase in body weight was also observed at 24 months. The objective of this study was to observe the changes in monoamine oxidase activity, lipid peroxidation levels and lipofuscin accumulation occurring in aging rat brain regions, and to see whether these changes are restored to normal levels after exogenous administration of DHEA (30 mg/kg/day for 1 month). The results obtained in the present work revealed that normal aging was associated with significant increases in the activity of monoamine oxidase, lipid peroxidation levels and lipofuscin accumulation in brain regions of 4, 14 and 24 months age group male rats. The present study showed that DHEA treatment significantly decreased monoamine oxidase activity, lipid peroxidation and lipofuscin accumulation in brain regions of aging rats, the increased body weight at 24 months also decreased more than the age matched controls. It can therefore be suggested that DHEA's beneficial effects seemed to arise from its antioxidant, antiobesity, antilipofuscin, antilipidperoxidative and thereby anti-aging actions. The results of this study will be useful for pharmacological modification of the aging process and development of new drugs for age related disorders.

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“…In contrast, Kellogg and Frye (1999) reported levels of allopregnanolone in brain extracts from adult males of 3.3 ng/g; a concentration only slightly lower than that they reported for estrous females and comparable to that which they found for 3α-diol in males (3.2 ng/g). For the negative neurosteroid modulators, Robel and Baulieu (1995) estimated levels of ~1-5 ng/ g for DHEAS in the brains of the adult male rat and various strains of mice, while Kazihnitková et al, 2004 provided somewhat higher values for DHEAS of ~6.2 and 8.8 ng/g in male rat subcortex and cortex, respectively.…”

Section: Neurosteroid and Anabolic Androgenic Steroid Levels In The Cnsmentioning

confidence: 99%

Steroid modulation of GABAA receptor-mediated transmission in the hypothalamus: Effects on reproductive function

Henderson

2007

Neuropharmacology

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The hypothalamus, the seat of neuroendocrine control, is exquisitely sensitive to gonadal steroids. For decades it has been known that androgens, estrogens and progestins, acting through nuclear hormone receptors, elicit both organizational and activational effects in the hypothalamus and basal forebrain that are essential for reproductive function. While changes in gene expression mediated by these classical hormone pathways are paramount in governing both sexual differentiation and the neural control of reproduction, it is also clear that steroids impart critical control of neuroendocrine functions through nongenomic mechanisms. Specifically, endogenous neurosteroid derivatives of deoxycorticosterone, progesterone and testosterone, as well and synthetic anabolic androgenic steroids that are self-administered as drugs of abuse, elicit acute effects via allosteric modulation of γ-aminobutyric acid type A receptors. GABAergic transmission within the hypothalamus and basal forebrain is a key regulator of pubertal onset, the expression of sexual behaviors, pregnancy and parturition. Summarized here are the known actions of steroid modulators on GABAergic transmission within the hypothalamus/basal forebrain, with a focus on the medial preoptic area and the supraoptic/paraventricular nuclei that are known to be central players in the control of reproduction.

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Simultaneous determination of dehydroepiandrosterone, its 7-hydroxylated metabolites, and their sulfates in rat brain tissues

Cited by 17 publications

References 19 publications

Stimulation of oxidative energy metabolism in liver mitochondria from old and young rats by treatment with dehydroepiandrosterone (DHEA). A comparative study

Stimulation of oxidative energy metabolism in liver mitochondria from old and young rats by treatment with dehydroepiandrosterone (DHEA). A comparative study

Effect of dehydroepiandrosterone (DHEA) on monoamine oxidase activity, lipid peroxidation and lipofuscin accumulation in aging rat brain regions

Steroid modulation of GABAA receptor-mediated transmission in the hypothalamus: Effects on reproductive function

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