Simultaneous determination of danshensu, hydroxysafflor yellow A, rosmarinic acid, lithospermic acid, salvianolic acid B in water extract of mixed Salviae Miltiorrhizae Radix et Rhizoma and Carthami Flos by HPLC

Li, Yao; Guo, Zheng-Tai; Gong, Xingchu; Qu, Haibin

doi:10.4268/cjcmm20131101

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Chinese Pharmacopia (2020 Edition), hydroxysafflor yellow A is the indicator component of Honghua. In previous work, it is observed that the content of hydroxysafflor yellow A was higher than that of rosmarinic acid in the water extract of mixed Danshen and Honghua [23]. However, the content of hydroxysafflor yellow A in Danhong injection is very low.…”

Section: Chemical Constituents In Danhong Injectionmentioning

confidence: 91%

A High-Performance Liquid Chromatography Method for the Simultaneous Determination of Five Index Components in Danhong Injection

An,

Tian,

Wang

et al. 2023

AJAC

View full text Add to dashboard Cite

The purpose of this study was to establish a high-performance liquid chromatography (HPLC) method for the simultaneous determination of sodium danshensu, protocatechuic aldehyde, rosmarinic acid, salvianolic acid B, and 4-coumaric acid in Danhong injection. The chromatographic method employed was as follows: the column was a Welch Ultimate XB-C18 column (250 mm × 4.6 mm, 10 μm), the mobile phase was a gradient elution of 0.4% formic acid aqueous solution (A) and acetonitrile (B), the detection wavelengths were 280 nm for sodium danshensu, protocatechuic aldehyde, and salvianolic acid B and 326 nm for 4-coumaric acid and rosmarinic acid, the sample volume was 10 μL, the flow rate was 1.0 mL/min, and the column temperature was 35˚C. This method can realize the separation and determination of sodium danshensu, protocatechuic aldehyde, rosmarinic acid, salvianolic acid B, and 4coumaric acid within 50 minutes. The linear relationships of the five peak areas and their concentrations are good (R 2 > 0.9997). The precision RSD values are all less than 1.0%. The reproducibility RSD values are all less than 1.3%. The stability RSD values are all less than 2.2%. The recovery values ranged from 92.4% to 99.4%. This method is simple, accurate, and reproducible. It can be used for the determination of sodium danshensu, protocatechuic aldehyde, rosmarinic acid, salvianolic acid B, and 4-coumaric acid in Danhong injection.

show abstract

Section: Chemical Constituents In Danhong Injectionmentioning

confidence: 91%

A High-Performance Liquid Chromatography Method for the Simultaneous Determination of Five Index Components in Danhong Injection

An,

Tian,

Wang

et al. 2023

AJAC

View full text Add to dashboard Cite

show abstract

Design Space Development for the Extraction Process of Danhong Injection Using a Monte Carlo Simulation Method

Gong

Chen

et al. 2015

PLoS ONE

View full text Add to dashboard Cite

A design space approach was applied to optimize the extraction process of Danhong injection. Dry matter yield and the yields of five active ingredients were selected as process critical quality attributes (CQAs). Extraction number, extraction time, and the mass ratio of water and material (W/M ratio) were selected as critical process parameters (CPPs). Quadratic models between CPPs and CQAs were developed with determination coefficients higher than 0.94. Active ingredient yields and dry matter yield increased as the extraction number increased. Monte-Carlo simulation with models established using a stepwise regression method was applied to calculate the probability-based design space. Step length showed little effect on the calculation results. Higher simulation number led to results with lower dispersion. Data generated in a Monte Carlo simulation following a normal distribution led to a design space with a smaller size. An optimized calculation condition was obtained with 10000 simulation times, 0.01 calculation step length, a significance level value of 0.35 for adding or removing terms in a stepwise regression, and a normal distribution for data generation. The design space with a probability higher than 0.95 to attain the CQA criteria was calculated and verified successfully. Normal operating ranges of 8.2-10 g/g of W/M ratio, 1.25-1.63 h of extraction time, and two extractions were recommended. The optimized calculation conditions can conveniently be used in design space development for other pharmaceutical processes.

show abstract

Simultaneous determination of danshensu, hydroxysafflor yellow A, rosmarinic acid, lithospermic acid, salvianolic acid B in water extract of mixed Salviae Miltiorrhizae Radix et Rhizoma and Carthami Flos by HPLC

Cited by 2 publications

References 0 publications

A High-Performance Liquid Chromatography Method for the Simultaneous Determination of Five Index Components in Danhong Injection

A High-Performance Liquid Chromatography Method for the Simultaneous Determination of Five Index Components in Danhong Injection

Design Space Development for the Extraction Process of Danhong Injection Using a Monte Carlo Simulation Method

Contact Info

Product

Resources

About