Simultaneous determination of danofloxacin and N-desmethyldanofloxacin in cattle and chicken edible tissues by liquid chromatography with fluorescence detection

Strelevitz, Timothy J.; Linhares, Michael C.

doi:10.1016/0378-4347(95)00354-1

Cited by 31 publications

(17 citation statements)

References 15 publications

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“…Plasma samples were stored at approximately Ϫ20°C prior to assay. Measurement of concentrations of danofloxacin and its active metabolite N-desmethyldanofloxacin in plasma was performed by using a validated high-pressure liquid chromatography method with solid-phase extraction and fluorescence detection (20). The limit of quantification (LOQ) was 10 ng/ml.…”

Section: Methodsmentioning

confidence: 99%

Pharmacokinetic and Pharmacodynamic Profiles of Danofloxacin Administered by Two Dosing Regimens in Calves Infected with Mannheimia ( Pasteurella ) haemolytica

Sarasola¹,

Lees

Aliabadi

et al. 2002

Antimicrob Agents Chemother

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The pharmacokinetics and pharmacodynamics of danofloxacin in calves with induced Mannheimia (Pasteurella) haemolytica pneumonia were evaluated. Calves received either saline as an intravenous (IV) bolus or danofloxacin (0.738 mg/kg of body weight) administered as either a single IV bolus or a 36-h continuous IV infusion. Blood samples and bronchial secretions were collected before and at predetermined times over 48 h following the start of treatment. Calves were assessed clinically throughout, and lung consolidation was assessed at necropsy. Bronchial secretions and lung tissue were cultured for M. haemolytica. Bolus administration of danofloxacin produced a high maximum drug concentration-to-MIC ratio (C max :MIC) of 14.5 and a time period of 9.1 h when plasma danofloxacin concentrations exceeded the MIC (T>MIC). Danofloxacin is a fluoroquinolone antimicrobial drug with rapid bactericidal activity against a broad range of pathogens responsible for a number of disease syndromes of economic importance in the commercial rearing of livestock (8,15). Since their introduction in the late 1980s, fluoroquinolones have been shown by a number of studies to exhibit concentration-dependent bactericidal activity, whereby the optimal effect is attained by the administration of high doses over a short period (4,6,14). This is a property shared by the aminoglycosides but is in contrast to the predominantly time-dependent bactericidal action shown by the ␤-lactam antibiotics (3), where the time that bacteria are exposed to antimicrobial concentrations exceeding the MIC (TϾMIC) is the major determinant of efficacy. These different types of action have been confirmed for danofloxacin and amoxicillin in an in vitro pharmacodynamic model against Actinobacillus pleuropneumoniae (9).The purpose of this study was to establish the pharmacokinetic and pharmacodynamic properties of danofloxacin in vivo by using an experimental model of calf pneumonia and to determine whether the concentration-dependent activity of danofloxacin in cattle operates under simulated clinical conditions. A fixed equal total dose of danofloxacin was administered either as a single intravenous (IV) bolus or by continuous infusion over a 36-h period, and the clinical and bacteriological outcomes in calves with induced infections of Mannheimia (Pasteurella) haemolytica were compared. The study was conducted in compliance with Good Clinical Practice guidelines (5), the analysis of samples was conducted in accordance with Good Laboratory Practice guidelines (16), and the husbandry of all animals was in compliance with the requirements of national legislation and local animal welfare guidelines. The study was conducted under veterinary supervision, with veterinary attention available at all times. MATERIALS AND METHODSAnimals. Thirty-three male Friesian calves (approximately 11 to 13 weeks of age, with initial body weights of 66.5 to 106 kg) were enrolled in the study and inoculated with M. haemolytica. Prior to enrollment, the calves were free from preexisting medic...

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Section: Methodsmentioning

confidence: 99%

Pharmacokinetic and Pharmacodynamic Profiles of Danofloxacin Administered by Two Dosing Regimens in Calves Infected with Mannheimia ( Pasteurella ) haemolytica

Sarasola¹,

Lees

Aliabadi

et al. 2002

Antimicrob Agents Chemother

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“…Phosphoric acid is frequently used as an extraction solvent for some types of polar or charged analytes being isolated from media such as plasma or tissue [20]. Its ability to remove the analyte from a protein bound state suggested that it would be a logical choice to remove these analytes from tissue.…”

Section: Discussionmentioning

confidence: 99%

Quantification of Paraquat, MPTP, and MPP+ in brain tissue using microwave-assisted solvent extraction (MASE) and high-performance liquid chromatography–mass spectrometry

et al. 2009

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Animal models, consistent with the hypothesis of direct interaction of paraquat (PQ) and 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) with specific areas of the central nervous system have been developed to study Parkinson’s disease (PD) in mice. These models have necessitated the creation of an analytical method for unambiguous identification and quantitation of PQ and structurally similar MPTP and 1-Methyl-4-phenylpyridinium ion (MPP+) in brain tissue. A method for determination of these compounds was developed using microwave-assisted solvent extraction (MASE) and liquid chromatography-mass spectrometry. Extraction solvent and microwave conditions such as power and time were optimized to produce recoveries of 90% for PQ 78% for MPTP and 97% for its metabolite MPP+. The chromatographic separation was performed on a C8, column and detection was carried out using an ion trap as an analyzer with electrospray ionization. Mass spectrometer parameters such as heated capillary temperature, spray voltage, capillary voltage and others were also optimized for each analyte. Analysis was done in Selective Ion Monitoring (SIM) mode using 186 m/z for PQ, m/z 174 for MPTP, and m/z 170 for MPP+. The method detection limit for paraquat in matrix was 100 pg and 40 pg for MPTP and 20 pg MPP+.

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“…Equation [4] can be written as: Table II, indicate the goodness of fit of the experimental points to the linear model predicted by equation [9]. This suggests the possibility of making k' predictions from just two experimental measurements of k' for each compound and the pKa values.…”

Section: Mobile Phase Optimisationmentioning

confidence: 99%

“…Many analytical methods for quinolone determination in biological tissues, both as veterinary residues or in the course of pharmacokinetic studies, have been developed. Most of them are based on a liquid-chromatographic separation in the reversed phase mode [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Optimisation of mobile phase pH in liquid chromatography. Application to fluorimetric detection of series of quinolones

et al. 1998

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SummaryThe dissociation constants of several quinolones in a 7 % acetonitrite-water mixture are determined. A model describing the effect of pH on the retention of quinolones by an octadecylsilica stationary phase with a 7 % acetonitrile-water mobile phase is proposed. The model uses pH values in the mobile phase instead of pH values in water and takes into account the effect of activity coefficients. Fluorescence of quinolones in the mobile phase is studied, and a chromatographic separation with fluorimetric detection is established. Detection limits for the proposed method range from 3 to 12 gg.L q.

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Simultaneous determination of danofloxacin and N-desmethyldanofloxacin in cattle and chicken edible tissues by liquid chromatography with fluorescence detection

Cited by 31 publications

References 15 publications

Pharmacokinetic and Pharmacodynamic Profiles of Danofloxacin Administered by Two Dosing Regimens in Calves Infected with Mannheimia ( Pasteurella ) haemolytica

Pharmacokinetic and Pharmacodynamic Profiles of Danofloxacin Administered by Two Dosing Regimens in Calves Infected with Mannheimia ( Pasteurella ) haemolytica

Quantification of Paraquat, MPTP, and MPP+ in brain tissue using microwave-assisted solvent extraction (MASE) and high-performance liquid chromatography–mass spectrometry

Optimisation of mobile phase pH in liquid chromatography. Application to fluorimetric detection of series of quinolones

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