Simultaneous determination of d- and l-amino acids in the nervous tissues of crustaceans using precolumn derivatization with (+)-1-(9-fluorenyl)ethyl chloroformate and reversed-phase ion-pair high-performance liquid chromatography

Okuma, Emiko; Abe, Hiroki

doi:10.1016/0378-4347(94)00304-1

Cited by 67 publications

(24 citation statements)

References 19 publications

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“…6) The HPLC methods using various chiral derivatizing reagents (including 1-fluoro-2,4-dinitrophenyl-5-Lalanine amide (FDAA, Marfey's reagent), [37][38][39] (ϩ)-1-(9-fluorenyl)ethyl chloroformate (FLEC), 40,41) o-phthalaldehyde (OPA) in combination with chiral thiols 22,[40][41][42][43][44][45][46] ) are also widely used because of its convenience and high resolution. The HPLC methods using chiral stationary phases (Pirkletype columns, [47][48][49] cyclodextrin bonded columns 50,51) ) are also useful.…”

Section: Analytical Methods For the Determina-tion Of D-amino Acids Imentioning

confidence: 99%

Sensitive Determination of D-Amino Acids in Mammals and the Effect of D-Amino-Acid Oxidase Activity on Their Amounts

Hamase

Konno

Morikawa

et al. 2005

Biological & Pharmaceutical Bulletin

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Section: Analytical Methods For the Determina-tion Of D-amino Acids Imentioning

confidence: 99%

Sensitive Determination of D-Amino Acids in Mammals and the Effect of D-Amino-Acid Oxidase Activity on Their Amounts

Hamase

Konno

Morikawa

et al. 2005

Biological & Pharmaceutical Bulletin

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“…Sardine muscle and viscera weighing 500 g were homogenized with 125 g of sodium chloride and incubated at 303C for a year. The preparation was well mixed daily and sampled once a month for creatine and creatinine determination after perchloric acid extraction as described previously (Okuma and Abe, 1994b).…”

Section: Preparation Of Fish Saucementioning

confidence: 99%

Chemical Composition of Fish Sauces Produced in Southeast and East Asian Countries

Park

Fukumoto

Fujita

et al. 2001

Journal of Food Composition and Analysis

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126

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“…13,14) Determination of Inorganic Ions and Osmolality Means with the same horizontal line that do not share a common superscript letter were significantly different (p<0.05 or less).…”

Section: Derivatization and Hplc Determination Of D And L-ami No Acidsmentioning

confidence: 99%

Effects of Seawater Acclimation on the Levels of Free D- and L-Alanine and Other Osmolytes in the Japanese Mitten Crab <i>Eriocheir japonicus</i>

et al. 1999

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During artificial seawater acclimation in the laboratory of freshwater Japanese mitten crab Eriocheir japonicus, glycine and L-proline other than D and L-alanine increased significantly in the mus cle of the immature group, while no significant increase in glycine and L-proline was found in the ma ture group. Total inorganic ions in the muscle of the mature crabs increased significantly along with the salinity increments and reached the levels of estuarine specimens captured during downstream migra tion. In the immature group, inorganic ions also increased during salinity stress but the increase was far lower than those in the mature group. These data suggest that immature crabs cannot tolerate high ion ic concentrations in muscle and have to increase glycine and L-proline during seawater acclimation. Af ter adjustment of salinity tolerance, mature crabs incorporate inorganic ions in muscle and their cell volumes are likely regulated mainly by D and L-alanine and inorganic ions.Hemolymph ion concentrations and osmolalities increased largely during seawater acclimation for both immature and mature crabs and reached the same levels as those for estuarine and sea specimens in the natural environment. These data suggest that no special adjustment of hemolymph osmoregulato ry capability occurs along with the maturation.Key words: D-alanine, L-alanine, crustacean, Eriocheir japonicus, hemolymph, muscle, os moregulation, seawater acclimationAs we proposed previously,1-4) free D-alanine accumulat ed in large amount in the tissues of various crustaceans and mollusks is thought to be the most potent osmolyte for intracellular isosmotic regulation. The levels of this specific amino acid are well regulated in the tissues of these invertebrates through alanine racemase action.5,6) Several species of crustaceans have been known to be strong os moregulators and to migrate freely between freshwater and seawater. A representative of these euryhaline crusta ceans is the Japanese mitten crab Eriocheir japonicus which inhabits the river for growth and maturation and mi grates downstream to the sea for mating and spawning. A closely related Chinese counterpart E. sinensis has long been employed for the studies on the tissue isosmotic regu lation as a classical model animal from the early 1950s.7-12) These catadromous species are known to exhibit an ex tremely high capability to regulate intracellular and ex tracellular osmolality. Thus, we were interested in the mechanisms underlying the powerful hyper-osmoregula tion of this species with special reference to the physiologi cal functions of free D-alanine.In the previous paper,13) we reported that the Japanese mitten crab accumulates only D and L-alanine and inorgan ic ions in muscle during maturation in the river and during downstream migration to estuaries, and accumulates more D and L-alanine and glycine in place of deleterious inor ganic ions in the sea. We concluded from these data that D and L-alanine are not simple osmolytes but play an im portant role in the adjustment of sa...

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Simultaneous determination of d- and l-amino acids in the nervous tissues of crustaceans using precolumn derivatization with (+)-1-(9-fluorenyl)ethyl chloroformate and reversed-phase ion-pair high-performance liquid chromatography

Cited by 67 publications

References 19 publications

Sensitive Determination of D-Amino Acids in Mammals and the Effect of D-Amino-Acid Oxidase Activity on Their Amounts

Sensitive Determination of D-Amino Acids in Mammals and the Effect of D-Amino-Acid Oxidase Activity on Their Amounts

Chemical Composition of Fish Sauces Produced in Southeast and East Asian Countries

Effects of Seawater Acclimation on the Levels of Free D- and L-Alanine and Other Osmolytes in the Japanese Mitten Crab <i>Eriocheir japonicus</i>

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