Simultaneous determination of cytosine arabinoside and its metabolite, uracil arabinoside, in human plasma using hydrophilic interaction liquid chromatography with UV detection

Abstract

A practical high-performance liquid chromatography using a Cosmosil HILIC column and UV detection was developed for determining the concentrations of cytosine arabinoside (Ara-C) and uracil arabinoside (Ara-U), which is a major metabolite of Ara-C, in human plasma. This method was used to determine the plasma concentrations of Ara-C and Ara-U in a patient treated with high-dose Ara-C therapy for end-stage renal failure.

L‐Lysine‐derived highly selective stationary phases for hydrophilic interaction chromatography: Effect of chain length on selectivity, efficiency, resolution, and asymmetry

“…This approach is gaining attention for the separation of polar compounds as observed from the recent reviews and many stationary phases have been developed . HILIC is a very useful technique due to the increasing need to analyze polar molecules in different areas of science and industry, including such as pharmaceutical industries , toxicology , environmental sciences , food industry , and drug metabolism . The increasing number of fields in which the HILIC mode can be applied is due to its excellent separation ability for polar or hydrophilic compounds such as peptides , carbohydrates , pharmaceuticals , proteins , natural product extracts , and small polar molecules .…”