Simultaneous determination of cryptotanshinone, tanshinone I and tanshinone IIA in traditional Chinese medicinal preparations containing Radix salvia miltiorrhiza by HPLC

Shi, Zhihong; He, Jiantao; Yao, Tingting; Chang, Wen‐Bao; Zhao, Meiping

doi:10.1016/j.jpba.2004.11.015

Cited by 96 publications

(55 citation statements)

References 9 publications

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“…Analytical methods for the quantitative determination of the active tanshinones, tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone, in Salvia miltiorrhiza plants and medicinal preparations have been reported (Hu et al, 2005;Shi et al, 2005;Yuan et al, 2005;Liu et al, 2006;Yang et al, 2006;Zhou et al, 2006;Ma et al, 2007). Cryptotanshinone was metabolized to a major metabolite, tanshinone IIA, and trace amounts of several hydroxyl-and dihydroxyl-cryptotanshinone in rats (Li et al, 2005;Song et al, 2005;Hao et al, 2006a;Sun et al, 2007a,b).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone in rat plasma by liquid chromatography–tandem mass spectrometry: application to a pharmacokinetic study of a standardized fraction of Salvia miltiorrhiza, PF2401‐SF

Park

Kim

et al. 2008

Biomedical Chromatography

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A rapid, sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for the simultaneous determination of tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone, the active components of Salvia miltiorrhiza in rat plasma, was developed. After liquid-liquid extraction with tariquidar as an internal standard, tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone were eluted from an Atlantis dC18 column within 5 min with a mixture of methanol and ammonium formate (10 mm, pH 6.5; 85:15, v/v). The analytes were detected by an electrospray ionization tandem mass spectrometry in the selected reaction monitoring (SRM) mode. The standard curves were linear (r=0.999) over the concentration range of 0.25-80 ng/mL for tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone in rat plasma. The coefficients of variation and the relative errors of tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone for intra- and inter-assay at four quality control (QC) concentrations were 1.1-5.1% and -4.0-6.0%, respectively. The lower limit of quantification for tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone was 0.25 ng/mL from 100 microL of plasma. This method was successfully applied to the pharmacokinetic study of tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone after oral administration of PF2401-SF, the standardized fraction of Salvia miltiorrhiza enriched with tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone to male Sprague-Dawley rats.

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Section: Introductionmentioning

confidence: 99%

Simultaneous determination of tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone in rat plasma by liquid chromatography–tandem mass spectrometry: application to a pharmacokinetic study of a standardized fraction of Salvia miltiorrhiza, PF2401‐SF

Park

Kim

et al. 2008

Biomedical Chromatography

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“…Previously, several methods have been established for the quality evaluation of RRSM, including TLC (Li et al, 1993), HPLC (Ma et al, 2006;Liu et al, 2006;Shi et al, 2004Shi et al, , 2005, HPCE (Chen et al, 2005), and LC-MS (Zhang et al, 2005). Unfortunately, the abovementioned methods could only employ a few phenolic acids or diterpenoids separately as chemical markers to evaluate the quality of RRSM, and few reports have dealt with both the hydrophilic and lipophilic bioactive compounds (Zhou et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Determination of fifteen bioactive components in Radix et Rhizoma Salviae Miltiorrhizae by high‐performance liquid chromatography with ultraviolet and mass spectrometric detection

Cao

Wei

et al. 2007

Biomedical Chromatography

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A high-performance liquid chromatographic (HPLC) method coupled with ultraviolet (UV) and electrospray ionization time-of-flight mass spectrometry (ESI-TOF/MS) was established for simultaneous qualitative and quantitative determination of nine phenolic acids and six diterpenoids in Radix et Rhizoma Salviae Miltiorrhizae (RRSM). The optimal chromatographic conditions were achieved on a Zorbax C 18 column by gradient elution with 0.1% (v/v) aqueous formic acid and acetonitrile as mobile phase at the flow rate of 1.0 mL/min. The detection wavelength at 281 nm was chosen to determine the 15 bioactive components, namely danshensu (1), protocatechuic acid (2), protocatechuic aldehyde (3), caffeic acid (4), rosmarinic acid (5), lithospermic acid (6), salvianolic acid B (7), salvianolic acid A (8), salvianolic acid C (9); dihydrotanshinone I (10), cryptotanshinone (11), tanshinone I (12), methylene tanshiqunone (13), tanshinone IIA (14) and miltirone (15). Additionally, LC-ESI-TOF/MS was used to make definite identification of the constituents in samples in comparison with those reference compounds. The validation of the method included tests of linearity, sensitivity, repeatability, stability and recovery. The proposed method was successfully applied to quantify the 15 components in 21 samples; significant variations were demonstrated in the contents of the samples from diverse species and origins. The developed method could be used to effectively and comprehensively evaluate the quality of RRSM for its clinical safety and efficacy.

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“…Because of its better performance and fewer side effects confirmed during the long-time clinical use, Danshen is widely used in traditional Chinese medicinal preparations to treat a number of coronary heart diseases, especially angina pectoris and myocardial infarction. [1][2][3][4] At present, a variety of Danshen products are commercially available, such as Fufang Danshen Tablets and Fufang Danshen Dripping pills, which are the two most commonly used ones in China and these have been officially listed in the Chinese Pharmacopoeia 2005. …”

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confidence: 99%

Pharmacokinetic Interaction between Tanshinones and Polyphenolic Extracts of Salvia miltinorrhiza BUNGE after Intravenous Administration in Rats

Guo

Zhang

Tang

et al. 2008

Biological & Pharmaceutical Bulletin

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The dry roots of Salvia miltiorrhiza, also called "Danshen" in Chinese, are a well-known traditional Chinese medicinal herb. Because of its better performance and fewer side effects confirmed during the long-time clinical use, Danshen is widely used in traditional Chinese medicinal preparations to treat a number of coronary heart diseases, especially angina pectoris and myocardial infarction. [1][2][3][4] At present, a variety of Danshen products are commercially available, such as Fufang Danshen Tablets and Fufang Danshen Dripping pills, which are the two most commonly used ones in China and these have been officially listed in the Chinese Pharmacopoeia 2005. 5)According to their chemical structures, the major bioactive constituents in S. miltiorrhiza BUNGE can be classified into two groups: the tanshinone compounds, which belong to a group of lipid-soluble diterpenoids, and water-soluble polyphenolic compounds.6) The major lipophilic components are cryptotanshinone, tanshinone I and tanshinone IIA (TSIIA). Pharmacological tests have revealed that TSIIA possesses a variety of pharmacological activities including antioxidant effects, 7) prevention of angina pectoris and myocardial infarction, 8) as well as antineoplastic, 9) anticancer, 10,11) and anti-inflammatory effects.12) The major hydrophilic components include danshensu, protocatechuic aldehyde and salvianolic acid B (Sal B). Sal B is the most common component in the Salvia species and the most abundant in their aqueous extracts. 13,14) Different studies have shown that Sal B has a variety of biological activities including anti-oxidation, 15,16) antifibrotic, 17) and myocardial salvage effects 18) ; it also prevents hepatitis and uremia, 19) and improves blood circulation and renal function. [20][21][22][23][24][25] Considering their prominent pharmacological activities and high content in Danshen, both TSIIA and Sal B have been selected as marker components for the quality control of Danshen and many other medicinal preparations containing Danshen in the 2005 edition of Chinese Pharmacopoeia. 6,12,26) The chemical structures of TanIIA and Sal B are shown in Fig. 1. Recently, Song et al. 27) showed that the pharmacokinetics of cryptotanshinone and TSIIA in rats after oral intra-gavage of an extract of tanshinones was significantly affected by the co-present tanshinones. Also, Sal B had a lower effective concentration in vitro angiogenesis study than the crude extract. 28) In clinical practice, most Danshen products used consist of tanshinone and polyphenolic extracts at the same time.5) However, there have been no reports of pharmacokinetic interactions between tanshinones and polyphenolic extracts of S. miltiorrhiza BUNGE in vivo.To date, several methods have been established for the de- The objective of this study was to investigate the interaction between tanshinones and polyphenolic extracts of Salvia miltiorrhiza BUNGE in rats. The rats in the medium dose groups were given an intravenous administration of 10 mg/kg tanshinones extract-loaded emulsi...

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Simultaneous determination of cryptotanshinone, tanshinone I and tanshinone IIA in traditional Chinese medicinal preparations containing Radix salvia miltiorrhiza by HPLC

Cited by 96 publications

References 9 publications

Simultaneous determination of tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone in rat plasma by liquid chromatography–tandem mass spectrometry: application to a pharmacokinetic study of a standardized fraction of Salvia miltiorrhiza, PF2401‐SF

Simultaneous determination of tanshinone I, dihydrotanshinone I, tanshinone IIA and cryptotanshinone in rat plasma by liquid chromatography–tandem mass spectrometry: application to a pharmacokinetic study of a standardized fraction of Salvia miltiorrhiza, PF2401‐SF

Determination of fifteen bioactive components in Radix et Rhizoma Salviae Miltiorrhizae by high‐performance liquid chromatography with ultraviolet and mass spectrometric detection

Pharmacokinetic Interaction between Tanshinones and Polyphenolic Extracts of Salvia miltinorrhiza BUNGE after Intravenous Administration in Rats

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