Simultaneous determination of creatinine and uric acid in urine by liquid chromatography–tandem mass spectrometry with polarity switching electrospray ionization

Determination of markers of the urinary stone disease

“…Among the hybrid methods for the determination of the concentration of USD markers the most wide spread are chromatographic [37][38][39][40] and electro phoretic [40][41][42][43][44][45][46] methods using a wide range of detec tors. Thus, for the determination of calcium and mag nesium ions in urine, ion chromatography with a piezoelectric detector was used [37].…”

“…On a cation exchange column 15 cm in length (5 × 150 mm) using a mobile phase with an addition of tartaric acid and ethylendiamine (pH 4.0), the elution times of magne A similar method of the preparation of urine samples consisting of centrifugation and filtration stages was proposed for the determination of uric acid by ion chromatography with a conductometric detec tor in the isocratic mode [38]. The simultaneous determination of creatinine and uric acid was recommended to perform using HPLC with mass spectrometric [39] or ultraviolet detection [40]. Using tandem mass spectrometry as one of the most sensitive methods of analysis, low limits of detec tion (10 mg/L for creatinine and 5 mg/L for uric acid) and low elution times of analytes (1.2 min for creati nine and 1.7 min for uric acid) were achieved.…”

“…Using tandem mass spectrometry as one of the most sensitive methods of analysis, low limits of detec tion (10 mg/L for creatinine and 5 mg/L for uric acid) and low elution times of analytes (1.2 min for creati nine and 1.7 min for uric acid) were achieved. The reversed phase version and gradient mode are used [39].…”

Determination of urine ionic composition with potentiometric multisensor system

“…Classical chromatography (liquid and gas) can be successfully applied for analysis of nonionic substances [16][17][18]. Creatinine and urate as uric acid can be quantified with liquid chromatography-tandem mass spectrometry in urine samples [16,17]. The derivatization with 2,3,4,5,6-pentafluorobenzyl bromide is used for creatinine determination in urine sample by gas chromatography-tandem mass spectrometry [18].…”

Simultaneous determination of N1-methylnicotinamide, l-carnitine, and creatinine in human plasma and urine by liquid chromatography with mass spectrometry for assessing the activities of multiple renal cationic transporters

“…A number of analytical methods for endogenous cations using high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) detection have been published recently [8][9][10][11][12]. LC-MS/MS system has many advantages (e.g., sensitivity and specificity) compared to the single mass spectrometry detection system (LC-MS).…”