Simultaneous determination of common phenolic acid in leaves of Avicennia officinalis

Abstract

The mangrove plant, Avicennia officinalis (L.), has long been utilized as an antioxidant, anti-inflammatory, and anticancer agent. In this study, p-coumaric acid and cinnamic acid were employed to simultaneously quantify common phenolic acids from leaf extract using a high-performance liquid chromatography method and a diode array detector. With a gradient elution of 0.1% formic acid in acetonitrile, 0.2% ammonium acetate/0.1% formic acid in the water, and methanol at a flow rate of 1.0 mL/min, chromatographic separation was performed on a Kromasil C18 column (150 mm × 4.6 mm i.d., 5 µm); detection was carried out at 280 nm. The limits of detection (LOD) and quantification (LOQ), respectively, were 0.025 mg/mL and 0.05 mg/mL. The recovery varied between 92.16 and 104.69%, whereas the relative standard deviations of intra-and inter-day analyses ranged from 1.71 to 3.22%. The validated approach was effectively used to analyze the bioactive ingredients in A. officinalis, and it could be a helpful process for maintaining the quality of this plant. Additionally, the bioactive substances found in the plant's leaves have a great deal of potential to become a source of traditional Vietnamese medicine.

Establishment of a standardized ethanolic extract of Avicennia officinalis leaves in Vietnam

“…The majority of this plant's distribution is in Malaysia, Bangladesh, India, and Indonesia. This species may be found in abundance in Vietnam's mangrove regions, particularly in the coastal provinces of Ca Mau, Kien Giang, and Tra Vinh (Thu et al, 2019;Duong et al, 2022) (Figure 1).…”