Simultaneous Determination of Cinnarizine and Dimenhydrinate in Binary Mixture Using Chromatographic Methods

Abstract

Two accurate and sensitive chromatographic methods have been developed and validated for simultaneous determination of cinnarizine (CIN) and dimenhydrinate (DIM). The first method uses simultaneous quantitative thin layer chromatography (TLC) spectrodensitometric evaluation of them, using ethyl acetate:methylene chloride (8 : 2 by volume) as a mobile phase. Chromatograms are scanned at 254 nm. This method analyzes CIN in a concentration range of 0.5-6 µg per band with mean percentage recovery of 99.78 ± 1.001 and DIM in a concentration range of 1-6 µg per band with mean percentage recovery of 99.87 ± 1.319. The second method is high-performance liquid chromatography using methanol:acetonitrile:water [85 : 10 : 5, by volume +0.5% tri ethyl amine (TEA)] as a mobile phase. The linearity was found to be in the range of 10-60 and 5-60 µg mL(-1) for CIN and DIM, respectively. The methods were successfully applied to the simultaneous determination of CIN and DIM in bulk powder, laboratory-prepared mixtures and pharmaceutical dosage forms. The validity of results was assessed by applying standard addition techniques. The results obtained are found to agree statistically with those obtained by a reported method, showing no significant difference with respect to accuracy and precision.

Bioanalysis of antihistamines for clinical or forensic purposes

“…Ali ( (Fusari et al, 1975;Argekar and Powar, 1999;Makhija and Vavia, 2001;Subramaniyan and Das, 2004;Czerwinska et al, 2013;Lamie and Monir, 2016 Hassan et al, 2002) or after proteolysis in the case of plasma or serum (Pandya et al, 1996). Hydrolysis of urine samples with β-glucuronidase (Dell and Fiedler, 1977) can also be performed.…”

“…Published studies based on TLC determination of antihistamines (chlorpheniramine, deiphenidramine, hydroxyzine, meclizine, triprolidine, cinnarizine and cetirizine) in biological specimens are limited and rather old (Table ). TLC methods that detect specific antihistamines in pharmaceutical formulations, alone or in combination with other drugs, have also been described (Fusari et al , ; Argekar and Powar, ; Makhija and Vavia, ; Subramaniyan and Das, ; Czerwinska et al , ; Lamie and Monir, ).…”

Experimentally designed chromatographic method for the simultaneous analysis of dimenhydrinate, cinnarizine and their toxic impurities

“…In addition to its lower detection limit and wider linearity range relative to the reported ones. 30,31,35 Moreover, the developed method is greener when compared with the reported method. 30 On the other hand, using of the experimental design during the optimization of the developed method saved time, effort and money.…”

Simultaneous estimation of dimenhydrinate, cinnarizine and their toxic impurities benzophenone and diphenylmethylpiperazine; in silico toxicity profiling of impurities

“…[26][27][28] Similarly, CIN was determined spectrophotometrically, 29,30 electrochemically 31,32 and by different chromatographic methods [33][34][35][36][37][38][39] either alone or in combination with other drugs and related impurities. On the other hand, DMH and CIN were simultaneously assayed by spectrophotometric, 40,41 TLC-densitometric 42,43 and HPLC 43,44 methods. Moreover, a ternary mixture of DMH, CIN and DPP was analyzed by TLC-densitometric 45 and HPLC 45 methods.…”