Simultaneous determination of cinnamaldehyde, eugenol and piperine by HPTLC densitometric method

Abstract

An HPTLC densitometric method for the simultaneous determination of cinnamaldehyde and eugenol as well as trace amounts of piperine in pepper-contaminated cinnamon was developed. The applicability of the method was tested with cinnamon bark powder adulterated with pepper powder, cinnamon oil, clove powder, clove oil and a commercial preparation containing cinnamaldehyde and eugenol. The method was validated for specificity, precision, accuracy and robustness. The method was found to be precise for different concentrations of cinnamaldehyde, eugenol and piperine. The accuracy of the method was checked by conducting a recovery study at three different levels. The linearity was found to be in the ranges 52.54-735.56, 533.2-8531.2 and 50-300 ng/spot, respectively, with correlation coefficients of 0.9985 +/- 0.04, 0.9982 +/- 0.06 and 0.9937 +/- 0.11 for cinnamaldehyde, eugenol and piperine.

Aqueous Cinnamon Extract (ACE-c) from the bark of Cinnamomum cassiacauses apoptosis in human cervical cancer cell line (SiHa) through loss of mitochondrial membrane potential

“…The supernatant was further filter-sterilized using swiney filter (pore size, 0.45 μm) and the resultant filtrate was stored in aliquots at -80°C until use. The bark identity was further confirmed by detecting the marker molecule cinnamaldehyde in ACE- c by HPTLC analysis as described previously [23,26]. The total polyphenol content of ACE-c was measured by Folin-Ciocalteau method as described previously [23,26].…”

Quantification and Brain Targeting of Eugenol-Loaded Surface Modified Nanoparticles Through Intranasal Route in the Treatment of Cerebral Ischemia

“…For PK studies, it is very important to look for an easy, simple and sensitive analytical method of quantification for EUG in brain and blood samples. Though various methods of EUG quantifications [44][45][46][47], have been reported, however most of these studies reported a simultaneous quantification of EUG with other drugs at the same times. Some of the studies reported EUG as individually i. e. not in plasma samples [48,49] or brain samples [50].…”

Karakterisasi Minyak Atsiri dari Simplisia Basah Ranting dan Daun sebagai Alternatif Subtitusi Kulit Batang Cinnamomum burmannii Blume

“…Prosedur profiling dilakukan sesuai metode yang dijabarkan oleh Gopu, et al dengan sedikit modifikasi. 20 Tabel 1 menunjukkan perbandingan nilai rendemen minyak Cinnamomum burmannii Blume pada masing-masing jenis sampel, baik yang berasal dari Tawangmangu ataupun Purwokerto. Rendemen minyak Cinnamomum burmannii Blume yang berasal dari Purwokerto rata-rata memiliki nilai yang lebih tinggi dibandingkan Tawangmangu.…”