Simultaneous determination of chromium(<scp>III</scp>) complexes and chromium(<scp>VI</scp>) by fast protein anion-exchange liquid chromatography–atomic absorption spectrometry

Milačić, Radmila; Štupar, Janez

doi:10.1039/an9941900627

Cited by 48 publications

(25 citation statements)

References 18 publications

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“…Two main arguments are underscored to show that such speciation is pointless. The first one is that the Cr(VI) ingested is expected to be reduced in the acidic environment of the stomach [4], leading to negligible health hazards. Nevertheless, some studies in animals and humans have shown strong side effects after Cr(VI) oral administration like increased values of urinary Cr [5] or development of tongue and small intestine carcinoma [6,7].…”

Section: Introductionmentioning

confidence: 99%

Cr(VI) speciation in foods by HPLC-ICP-MS: investigation of Cr(VI)/food interactions by size exclusion and Cr(VI) determination and stability by ion-exchange on-line separations

2015

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A method has been developed for the specific and sensitive determination of Cr(VI) in foods. First, the interactions between Cr(VI) and the matrices were investigated by size-exclusion HPLC-ICP-MS (SEC-ICP-MS). Evidence was found for the complexation of Cr(VI) potentially present with the ligands. For quantification of Cr(VI), the method was based on an alkaline extraction (NH4OH solution at pH 11.5) followed by Cr(VI) determination by anion-exchange HPLC-ICP-MS. Analytical performances of the method were satisfactory in terms of linearity, specificity, accuracy, repeatability, and intermediate precision. Detection limits ranged from 1 to 10 μg/kg, depending on the matrices investigated. The method was then applied for the determination of Cr(VI) in several products (dairy products, flour, chocolate, vegetables, fruits, meat, fish, eggs, and beverages) from different brands and origins. Cr(VI) was found in none of the samples investigated. To further investigate the reason for this absence, a stability study of spiked Cr(VI) was therefore conducted. A semi-skimmed cow milk was selected for this study. Cr(VI) was shown to be unstable in this matrix with a degradation rate increasing with the temperature.

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Section: Introductionmentioning

confidence: 99%

Cr(VI) speciation in foods by HPLC-ICP-MS: investigation of Cr(VI)/food interactions by size exclusion and Cr(VI) determination and stability by ion-exchange on-line separations

2015

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“…Cr-EDTA and Cr-oxalate as well as Cr 3+ on the anion-exchange FPLC column was investigated in our previous work [25]. It was demonstrated that Cr-EDTA and Cr-oxalate were eluted from 6.0 to 7.0 min and 8.0 to 9.0 min, respectively, and were therefore completely separated from Cr(VI).…”

Section: Determination Of Cr(vi) In Standard Cro 4 2-solutions By Animentioning

confidence: 99%

“…After leaching, Cr(VI) was determined by ion chromatography or extraction with a liquid anion exchange solution of Amberlite LA 2/MIBK. Among different analytical techniques developed or modified in our laboratory for the determination of Cr(VI) in a variety of sample matrices [21,22,23,24] anion-exchange fast protein liquid chromatography (FPLC) with AAS detection offered simultaneous determination of Cr(III) complexes and Cr(VI) [25]. The technique was successfully applied in speciation of Cr in plant sap [25] and in the investigation of oxidation-reduction processes of trace amounts of Cr in highly alkaline lime-treated sewage sludge samples [26].…”

Section: Introductionmentioning

confidence: 99%

Determination of Cr(VI) in welding fumes by anion-exchange fast protein liquid chromatography with electrothermal atomic absorption spectrometric detection

2002

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The applicability of an anion-exchange fast protein liquid chromatographic-electrothermal atomic absorption spectrometric procedure (FPLC-ETAAS) was investigated for the determination of Cr(VI) in welding fumes after alkaline extraction of aerosols loaded on filters. Gas tungsten arc welding (GTAW) of stainless steel was applied. Samples of welding fumes were collected during regular welding on polycarbonate membrane filters of 8 microm and 0.4 microm pore size (inhalable and respirable aerosols). Alkaline extraction (2% NaOH-3% Na2CO3) of filters in a heated ultrasonic bath was applied to leach Cr from the airborne particulate matter. 0.5 cm3 of sample extract was then injected onto an anion-exchange FPLC column. Tris-HCl buffer (0.005 mol dm(-3), pH 8.0) and the same buffer with NaCl (0.5 mol dm(-3)) were employed in gradient elution (15 min, flow rate 1 cm3 min(-1)). The separated Cr species were determined "off line" by ETAAS in 0.5 cm3 fractions. Cr(VI) was reproducibly and quantitatively eluted from 12.0 to 13.0 min with a maximum peak at 12.5 min. Good repeatability of measurement (+/-3.0%) of alkaline extracts was obtained for Cr(VI). The LOD (3s) was found to be 0.035 microg m(-3) Cr(VI), when 2 m3 of aerosols were collected on the filter. Validation of the procedure was performed by spiking alkaline extracts and by the analysis of standard reference material CRM 545, Cr(VI) in welding dust loaded on a filter. The technique was successfully applied for the determination of Cr(VI) in welding fumes.

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“…Recently, it was reported that several ppm of chromium was measured in many livers following sudden traumatic death (2). Thus, interest in the possible biological function of chromium continues to increase, particularly at the trace level.Many researchers have presented the determination of chromium in biological materials by electrothermal atomic absorption spectrometry (ETAAS) (2-16), inductively coupled plasma emission spectrometry (ICP) (17), and flame AAS (18,19), which have relatively high sensitivities and are convenient. In these methods, ETAAS has higher sensitivity but severe chemical interferences (6,16).…”

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confidence: 99%

Atomization Characteristic of Chromium on a Molybdenum Tube Atomizer by Electrothermal Atomic Absorption Spectrometry and Determination of Chromium in Biological Materials

Ohta

Uegomori

Itoh

et al. 1997

Microchemical Journal

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Simultaneous determination of chromium(III) complexes and chromium(VI) by fast protein anion-exchange liquid chromatography–atomic absorption spectrometry

Cited by 48 publications

References 18 publications

Cr(VI) speciation in foods by HPLC-ICP-MS: investigation of Cr(VI)/food interactions by size exclusion and Cr(VI) determination and stability by ion-exchange on-line separations

Cr(VI) speciation in foods by HPLC-ICP-MS: investigation of Cr(VI)/food interactions by size exclusion and Cr(VI) determination and stability by ion-exchange on-line separations

Determination of Cr(VI) in welding fumes by anion-exchange fast protein liquid chromatography with electrothermal atomic absorption spectrometric detection

Atomization Characteristic of Chromium on a Molybdenum Tube Atomizer by Electrothermal Atomic Absorption Spectrometry and Determination of Chromium in Biological Materials

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