Simultaneous Determination of Chlorophylls, Pheophytins, β‐Carotene, Tocopherols, and Tocotrienols in Olive and Soybean Oils by High‐performance Liquid Chromatography

Lutein is a major carotenoid in soybean [Glycine max (L.) Merr.] seed, and has been shown to be beneficial for eye health in humans. Development of soybeans high in lutein is a goal of some breeding programmes. Little is known about how different growing environments affect lutein content. The objective of this study was to determine the variation of lutein and its relationship to seed protein, oil and individual fatty acids in soybean seed. Fifteen soybean genotypes were planted at four environments. There was no effect of year and planting date on lutein content in soybean seed. However, genotype · year, genotype · planting date and genotype · year · planting date were significant for lutein content. Although each genotype showed similar response across environments, lutein content varied significantly across the four growing environments in 14 of the 15 genotypes evaluated. Lutein content was not correlated with seed protein or oil and palmitic or stearic acid concentrations. However, lutein was positively correlated with oleic acid content and negatively correlated with linoleic and linolenic acids content.

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“…As a constituent in soybean oil (Seppanen et al. 2003), increasing lutein can improve the value of soybean oil.…”

Section: Resultsmentioning

confidence: 99%

Environmental effects on lutein content and relationship of lutein and other seed components in soybean

Lee

Shannon

et al. 2009

Plant Breeding

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“…In some cases, the direct solvent extraction (Mendoza, Pons, Bargallo, & Lopez-Sabater, 2003;Ryynänen, Lampi, Salo-Väänänen, Ollilainen, & Piironen, 2004) or the Soxhlet extraction (González, Pablos, Martín, León-Camacho, & Valdenebro, 2001;Ramadan & Moersel, 2002) of the vitamins, without previous saponification, is carried out. For the analysis of vitamin E in oils, the sample can be injected directly into the LC system after appropriate dilution in an organic solvent (Gliszczynska-Swiglo & Sikorska, 2004;Puspitasari-Nienaber, Ferruzzi, & Schwartz, 2002;Seppanen, Rahmani, & Csallany, 2003).…”

Section: A-tocopherol Contentmentioning

confidence: 99%

Effects of γ-irradiation on the α-tocopherol and fatty acids content of raw unpeeled almond kernels (Prunus dulcis)

Stefano

Pitonzo

Bartolotta

et al. 2014

LWT - Food Science and Technology

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The present study evaluated fatty acid composition and a-tocopherol content of almond as a function of irradiation dose in order to determine dose levels causing minimal undesirable changes to almonds. Raw unpeeled almonds variety Tuono (Prunus dulcis (Mill.) D. A. Webb) were irradiated using 60 Co source at dose of 0.5, 1.5, 3, 6, 8 and 10 kGy. Both control and irradiated samples were kept frozen and immediately analyzed. The data obtained showed no change in fatty acid compositions up to a dose of 10 kGy; on the contrary, a general trend observed is that increasing the dose of irradiation resulted in the decrease of a-tocopherol content. The study has shown that irradiation is an effective tool in simultaneous preservation of a-tocopherol, that is the main vitamin-E active compound, and fatty acids content in almonds at low doses.

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“…For all these reasons, it is not surprising that a great effort is dedicated to develop standardized methods to quantify pigments and possibly correlating them to specific features of EVOO production and quality. Several analytical methods based on high performance liquid chromatography (HPLC) have been proposed to identify and quantify carotenoids (Cortes et al, 2004), chlorophyll derivatives (Watanabe, 1984) or both (Seppanen, Rahmani, & Csallany, 2003;Minguez-Mosquera, Gandul-Rojas, & Gallardo-Guerrero, 1992). Most of these methods require a preventive solvent extraction of pigments from the olive oil lipid matrix.…”

Section: Introductionmentioning

confidence: 99%

Pigments in extra virgin olive oils produced in different mediterranean countries in 2014: Near UV-vis spectroscopy versus HPLC-DAD

Lazzerini

Cifelli

Domenici

2017

LWT

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Carotenoids and chlorophyll derivatives play a key role in Extra Virgin Olive Oils (EVOOs). Many factors,\ud such as cultivar, geographic origin, maturity of olives, climate and storage conditions, influence the\ud pigments’ content. The quantification of pigments is usually done by chromatographic techniques.\ud However, recent works evidenced the potentialities of UV-visible-related methodologies. In this\ud research, a selection of EVOO samples produced from olives harvested at the beginning of November\ud 2014 in Greece, Tunisia, Italy and Spain, was investigated in terms of pigments by means of two methods.\ud The first one is a recent approach based on the mathematical treatment of near UV-vis absorption spectra\ud of olive oils to quantify in a fast, cheap and non-destructive way four main pigments, namely b-carotene,\ud lutein, pheophytin A and pheophytin B. The second one is a more standard HPLC-DAD method. From the\ud comparison between the two methods, we can conclude that the new near UV-vis approach gives\ud reliable results, with good precision and high reproducibility. Pigments quantified by these two methods\ud in EVOOs produced in four countries from different cultivars are analyzed by principal component\ud analysis (PCA). Results indicate that pigments can be correlated to several factors such as ripeness stage,\ud geographic origin and cultivars

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Simultaneous Determination of Chlorophylls, Pheophytins, β‐Carotene, Tocopherols, and Tocotrienols in Olive and Soybean Oils by High‐performance Liquid Chromatography

Cited by 30 publications

References 11 publications

Environmental effects on lutein content and relationship of lutein and other seed components in soybean

Environmental effects on lutein content and relationship of lutein and other seed components in soybean

Effects of γ-irradiation on the α-tocopherol and fatty acids content of raw unpeeled almond kernels (Prunus dulcis)

Pigments in extra virgin olive oils produced in different mediterranean countries in 2014: Near UV-vis spectroscopy versus HPLC-DAD

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