Simultaneous Determination of Cefotaxime Sodium and Paracetamol by LC-MS

Chinnadurai, Sivakumar

doi:10.9790/3013-040201012-18

Cited by 4 publications

(4 citation statements)

References 13 publications

(9 reference statements)

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“…The developed methods were validated according to the international conference on harmonisation guidelines ICH [24].…”

Section: Methods Validationmentioning

confidence: 99%

“…According to a comprehensive survey, few analytical techniques have been employed like RP-HPLC and LC-MS for determination of CEF and PAR, 23,24 simultaneously. Elhassan et al developed a RP-HPLC method using 1% formic acid in methanol as a mobile phase was optimal with a lower flow rate of 0.8 mL/min.…”

Section: Introductionmentioning

confidence: 99%

“…Our developed method does not require buffer solution. 24 Our study presents a chromatographic approach that is characterized by its simplicity, speed, reproducibility and sensitivity. The method was developed for the accurate detection of CEF and PAR in a synthetic mixture.…”

Section: Introductionmentioning

confidence: 99%

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Developing a highly validated and sensitive HPLC method for simultaneous estimation of cefotaxime and paracetamol in pure and pharmaceutical preparations

Sebaiy,

El-Adl,

Elbaramawi

et al. 2024

10.5267/j.ccl

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An isocratic HPLC technique was exploited and validated for the quick simultaneous separation and measurement of cefotaxime and paracetamol in vials dosage forms, with a total analysis time of 3 minutes. The process of separation was carried out on a Thermo Scientific® Venusil XBPC18 (L) (5µm, 4.6x250 mm) using a mobile phase of ACN: distilled water (70:30, v/v) at the ambient temperature. The flow rate used in the experiment was 1 mL/min, and the highest level of absorption was determined by high-performance liquid chromatography with photodiode array detection (HPLC-PDA) employing a PDA detector set at a wavelength of 255 nm. The established retention times for cefotaxime and paracetamol were 1.79 and 2.97 minutes, respectively, suggesting reduced analysis duration. The observed limits of detection for ceftaxime and paracetamol were 4.2×10-5 and 1.2×10-5 µg/mL, respectively, indicating a significant level of sensitivity in the approach. The approach was subsequently verified in accordance with the requirements set out by the Food and Drug Administration (FDA) for the quantification of medicines in vial dosage form.

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“…The developed methods were validated according to the international conference on harmonisation guidelines ICH [24].…”

Section: Methods Validationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Developing a highly validated and sensitive HPLC method for simultaneous estimation of cefotaxime and paracetamol in pure and pharmaceutical preparations

Sebaiy,

El-Adl,

Elbaramawi

et al. 2024

10.5267/j.ccl

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“…Various methods for determining cefotaxime concentration have been developed as a single analyte or simultaneously with other cephalosporin antibiotics. The instruments used also vary, namely spectrophotometry UV [4], HPTLC [5], HPLC-UV [6][7][8][9][10][11][12], UHPLC-MS/MS [13,14], LC-MS [15], and UPLC-MS/MS [16].…”

Section: Preparation Of Standard Solutionsmentioning

confidence: 99%

Bioanalytical and validation high-performance liquid chromatography method for simultaneous quantification cefotaxime and ciprofloxacin in human plasma

Dewi,

Wahyono,

Puspitasari

et al. 2023

J Appl Pharm Sci

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This study aims to obtain a validated method for simultaneously determining cefotaxime and ciprofloxacin levels in spiked human plasma. The chromatographic system used a Luna Phenomenex ® C18 column (250 × 4 mm i.d; 5 µm), and a mobile phase consisting of an isocratic mixture of 0.02 M phosphate buffer (pH 3.0), acetonitrile (ACN), and methanol (80:12:8 v/v), flow rate 1.0 ml/minute, 280 nm. Extraction of human plasma was carried out using 100 µl of acetic acid 10% and ACN, and extraction was repeated twice, the supernatant obtained was collected and then evaporated to dryness. The dried sample was dissolved with 500 µl of mobile phase before being injected into the system. The method was validated by evaluating the selectivity, linearity, accuracy, precision, and stability parameters. This method was declared valid for the simultaneous determination of cefotaxime and ciprofloxacin levels in human plasma and it is stated to be linear with the value of the correlation coefficient of 0.9986 for cefotaxime and 0.9981 for ciprofloxacin. The average percent recovery of cefotaxime was in the range of 89.13 and 112.81. Meanwhile, ciprofloxacin was in the range of 94.33 and 107.98. The developed analytical method is stated to fulfill the validity requirements.

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Indirect spectrophotometric determination of cefotaxime using N-bromosuccinimide and crystal violet dye

Nayif

Alhabbo

2023

International Conference of Computational Methods in Sciences and Engineering Iccmse 2021

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Simultaneous Determination of Cefotaxime Sodium and Paracetamol by LC-MS

Abstract: ABSTACT:A rapid and sensitive liquid chromatography-mass spectrometry (LC-MS)

Cited by 4 publications

References 13 publications

Developing a highly validated and sensitive HPLC method for simultaneous estimation of cefotaxime and paracetamol in pure and pharmaceutical preparations

Developing a highly validated and sensitive HPLC method for simultaneous estimation of cefotaxime and paracetamol in pure and pharmaceutical preparations

Bioanalytical and validation high-performance liquid chromatography method for simultaneous quantification cefotaxime and ciprofloxacin in human plasma

Indirect spectrophotometric determination of cefotaxime using N-bromosuccinimide and crystal violet dye

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