Simultaneous determination of cefatrizine and clavulanic acid in dog plasma by HPLC

Choi, Hyun Seok; Jun, H. W.; Kim, Dae‐Duk; Sah, Hongkee; Yoo, Bong Kyu; Yong, Chul Soon

doi:10.1016/j.jpba.2004.01.010

Cited by 14 publications

(10 citation statements)

References 28 publications

(26 reference statements)

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“…Although clavulanic acid is known to be highly unstable in blood, Choi et al [21] showed that no significant change was detected in the concentration of clavulanic acid when stored at −80 • C for 3 months in plasma. We also found no significant reduction in the concentration of both amoxicillin and clavulanic acid when stored at −30 • C for 2 weeks in plasma.…”

Section: Methods Optimization and Validationmentioning

confidence: 99%

Simultaneous determination of amoxicillin and clavulanic acid in human plasma by HPLC–ESI mass spectrometry

Yoon¹,

Lee²,

Kim³

et al. 2004

Journal of Chromatography B

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Section: Methods Optimization and Validationmentioning

confidence: 99%

Simultaneous determination of amoxicillin and clavulanic acid in human plasma by HPLC–ESI mass spectrometry

Yoon¹,

Lee²,

Kim³

et al. 2004

Journal of Chromatography B

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“…The equation parameters (slope and intercept) of each standard curve were used to determine concentration values for unknown samples. Concentrations were backcalculated and compared with the nominal concentrations, and the relative concentration residuals (RCRs) were calculated (14).…”

Section: Linearitymentioning

confidence: 99%

Simultaneous Determination of Levocetirizine and Pseudoephedrine in Dog Plasma by Liquid Chromatography-Mass Spectrometry in the Presence of Dextrocetirizine

Ryu

Yoo

2012

J Pharm Pharm Sci

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Purpose. This study describes the development of a rapid and sensitive LC-ESI-MS assay for simultaneous enantioselective determination of levocetirizine and pseudoephedrine in dog plasma in the presence of dextrocetirizine. Methods. Separations were achieved on an Ultron ES-OVM chiral column using the mobile phase consisting of 10 mM aqueous NH 4 OAc (pH 6.6) and acetonitrile (9:1 v/v). Results. The retention times of pseudoephedrine, dextrocetirizine, levocetirizine and diazepam (internal standard) were 5.2, 8.3, 9.6 and 11.6 min, respectively, and the total run time was less than 15 min. The assay was validated to demonstrate the linearity, accuracy and precision, recovery and stability. The calibration curves were linear over the concentration range from 1 -200 ng/mL for levocetirizine and from 5 -1000 ng/mL for pseudoephedrine. Conclusions. The developed assay was successfully applied to a pharmacokinetic study after oral administration of the racemic cetirizine (0.5 mg/kg, or 0.25 mg/kg as levocetirizine) and pseudoephedrine (12 mg/kg) in the dog.

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“…Hoizey et al (2002) and Foroutan et al (2007) developed HPLC-UV methods for determining AMX and CLV in plasma, respectively. However, because of CLV's weak absorbance in ultraviolet wavelengths, a pre-column derivatization of CLV combined with UV-detection had to be used (Choi et al 2004). The post-column derivatization of CLV combined with fluorimetric detection was also reported (Haginaka, Yasuda, and Uno 1986).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Amoxicillin and Clavulanic Acid in Dog Plasma by High-Performance Liquid Chromatography Tandem Mass Spectrometry

Guo

et al. 2012

Analytical Letters

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A rapid, sensitive, and specific high-performance liquid chromatography tandem mass spectrometric method was developed for the simultaneous determination and confirmation of amoxicillin and clavulanic acid in plasma. Plasma sample was subjected to a simple deproteinization with acetonitrile, and then the supernatant was directly diluted by water. Analysis was performed on a Phenomenex Luna C 8 reversed-phase column by detection with mass spectrometry in negative ions multiple reaction monitoring mode. A gradient elution program with 0.1% formic acid and acetonitrile was performed at a flow of 0.25 mL min À1 . There is good linearity in the range of 0.5-500 ng mL À1 for both amoxicillin and clavulanic acid. The decision limits of amoxicillin and clavulanic acid were 0.06 ng mL À1 and 0.08 ng mL À1 in plasma, respectively, and the detection capabilities of two analytes were below 0.5 ng mL À1 . Acceptable precision and accuracy were obtained for concentrations over the standard curve range. The extraction recoveries of amoxicillin and clavulanic acid were between 102% and 115% in plasma at three spiked levels of 0.5, 50, and 500 ng mL À1 , with the relative standard deviations less than 15% for each analyte. The developed method was applied to pharmacokinetic studies of amoxicillin and clavulanic acid tablets in healthy beagles.

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Simultaneous determination of cefatrizine and clavulanic acid in dog plasma by HPLC

Cited by 14 publications

References 28 publications

Simultaneous determination of amoxicillin and clavulanic acid in human plasma by HPLC–ESI mass spectrometry

Simultaneous determination of amoxicillin and clavulanic acid in human plasma by HPLC–ESI mass spectrometry

Simultaneous Determination of Levocetirizine and Pseudoephedrine in Dog Plasma by Liquid Chromatography-Mass Spectrometry in the Presence of Dextrocetirizine

Simultaneous Determination of Amoxicillin and Clavulanic Acid in Dog Plasma by High-Performance Liquid Chromatography Tandem Mass Spectrometry

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