Simultaneous determination of catecholamines and metanephrines in urine by HPLC with fluorometric detection

Abeling, N.G.G.M.; Gennip, Albert H.; Overmars, H.; Voûte, P. A.

doi:10.1016/0009-8981(84)90181-5

Cited by 59 publications

(19 citation statements)

References 12 publications

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“…The recoveries (± SD) of 500 ng external Standards were (n = 13 each), normetanephrine: 93.5 + 5.4%; metanephrine: 82.7 ± 5.4%, and 3-methoxytyramine: 93.8 ± 9.0%. These values are in the same ränge of data that have appeared in the literature (10,11). Individuäl values are given in table l. It is our experience that acid äs well äs enzymatic hydrolysis increases the number of electrochemically detectable compounds in biological samples.…”

Section: Resultssupporting

confidence: 64%

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24 h Excretion Patterns of Free, Conjugated and Methylated Catecholamines in Man

Westerink¹,

Kate²

1986

Clinical Chemistry and Laboratory Medicine

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Siunmary:A method based on high performance liquid chromatography with electrochemical detection is described for quantification of adrenaline, noradrenaline, dopamine (free and total), metanephrine, normetanephrine, and 3-methoxytyramine (free) in urine samples. Purification of the samples is achieved on disposable -Rad ion-exchange columns in combination with small Sephadex G 10 columns. A clear circadian rhythm was found in the 24 h excretion pattern of free adrenaline and noradrenaline for 4 volunteers (city bus drivers during a working day). No rhythms were seen in the 24h excretion pattern of free or conjugated dopamine, conjugated adrenaline or conjugated noradrenaline, or of the methylated free catecholamines. It is concluded that in order to obtain Information about the neurotransmitter function of peripheral catecholamines it is sufficient to determine the non-conjugated compounds; values for the conjugated and methylated metabolites contribute no extra Information. 24-Stunden^-Ausscheidungsmuster der freien, konjugierten und methylierten Katecholamine beim MenschenZusammenfassung: Eine Methode zur quantitativen Bestimmung von Adrenalin, Noradrenalin, Dopamin (freies und gesamtes), Metajjephrin, Normetanephrin und 3-Methoxytyramin (freies) im Harn auf der Grundlage der Hochleistungsflüssigchromatographie mit elektrochemischer Detektion wird beschrieben. Die Reinigung der Proben wird durch Einmäl-Ionenaustauscher-Säulen ( -Rad) in Kombination mit kleinen Sephadex G -Säulen erreicht. Bei vier Busfahrern wurde während eines Arbeitstages ein deutlicher circadianer Rhythmus im 24^Stunden*Ausscheidungsmuster für freies Adrenalin und Noradrenalin gefunden. Für freies öder konjugiertes Dopamin, konjugiertes Adrenalin, konjugiertes Noradrenalin oder die methylierten freien Katecholamine wurde keine Rhythmik im 24-Stunden-Ausscheidungsmuster beobachtet. Wir schließen daraus, daß es für den Erhalt von Informationen über die Neurotransmitterfunktion der peripheren Katecholamine ausreicht, die nicht-konjügierten Verbindungen zu bestimmen; Werte für die konjugierten und methylierten Metabolite erbringen keine weitere Information.is some recent evidence that also methylated catecholIntroduetion amines should be taken into account äs indices of Psychoendocrine research has emphasized the use of sympathieoadrenal activity (5). It is believed that Ocätecholamines äs indicators of mental stress and methylation of catecholamines by the extraneuronally physical workload (l, 2). Adrenaline (A) excretion localized enzyme, catechol-O-methyl transferase, is appears to be particulary responsive to mental stress, the primary metabolic route for actively released catewhereas urinary noradrenaline responds mainly to cholamine. This enzyme converts noradrenaline, adphysical activity (3, 4). Although studies in this field renaline and dopamine into nonnetanephrine, metaare usually restricted to the free catecholamines, there nephrine, and 3-methoxytyramine, respectively.

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Section: Resultssupporting

confidence: 64%

“…However, these methods were usually developed for diagnosis of catecholamine secreting tumours, äs is the case in pheochromocytoma. In contrast to the total (free plus conjugated) urinary metanephrines, data on the levels of the free metanephrines are relatively scarce in the literature (10,11).…”

mentioning

confidence: 99%

24 h Excretion Patterns of Free, Conjugated and Methylated Catecholamines in Man

Westerink¹,

Kate²

1986

Clinical Chemistry and Laboratory Medicine

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“…Urine concentrations of catecholamines and their metabolites were determined at two centers: the NIH and the Academic Medical Center. These measurements were carried out using gas chromatography-mass spectroscopy at the NIH (8,28) and liquid chromatography with fluorimetric detection at the Academic Medical Center (29,30). Urine concentrations of 5-HT were also measured at the latter center using liquid chromatography with fluorometric detection.…”

Section: Methodsmentioning

confidence: 99%

Specific genetic deficiencies of the A and B isoenzymes of monoamine oxidase are characterized by distinct neurochemical and clinical phenotypes.

Lenders¹,

Eisenhofer²,

Abeling³

et al. 1996

J. Clin. Invest.

148

101

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Monoamine oxidase (MAO) exists as two isoenzymes and plays a central role in the metabolism of monoamine neurotransmitters. In this study we compared the neurochemical phenotypes of previously described subjects with genetically determined selective lack of MAO-A or a lack of both MAO-A and MAO-B with those of two subjects with a previously described X chromosome microdeletion in whom we now demonstrate selective MAO-B deficiency.

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“…Intra-and inter-assay variations, calculated on low, mid, and high levels, ranged from 1.2 to 7.8% (intra-assay) and 4.8-10.4% (inter-assay) respectively. Concentrations of HVA, VMA, MHPG, DA, and NE in urine were determined using RP-HPLC with ECD and fluorometric detection (Abeling et al, 1984;Stroomer et al, 1990). For HVA, VMA, and MHPG variation calculated on three different levels ranged from 1.2 to 4.1% (intraassay) and 3.6-8.5% (inter-assay) respectively.…”

Section: Catecholamine Metabolites and Prolactinmentioning

confidence: 99%

Disrupted Dopaminergic Neurotransmission in 22q11 Deletion Syndrome

Boot

Booij²,

Zinkstok

et al. 2007

Neuropsychopharmacol

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22q11 Deletion syndrome (22q11DS) is associated with chromosome 22q11 microdeletions and high rates of psychiatric disorders. Susceptibility for these disorders could be explained by haploinsufficiency of the catechol-O-methyltransferase gene, which encodes an enzyme involved in dopamine (DA) breakdown. It is unknown how dopaminergic neurotransmission is affected in people with 22q11DS. To date, there have been no controlled studies investigating dopaminergic neurotransmission in people with 22q11DS. We report the results of a challenge study in high-functioning adults with 22q11DS and age-and gender-matched controls using neuro-endocrine and peripheral dopaminergic markers. At baseline, 22q11DS subjects compared to controls had higher urine DA levels and lower plasma levels of the predominant DA metabolite homovanillic acid (HVA). Following DA depletion, 22q11DS subjects showed lower urine and plasma HVA levels and a lower prolactin response than controls. The ratio of DA/HVA, a rough index of DA turnover, was significantly higher in the 22q11DS subjects at baseline and after DA depletion. Our results suggest that adults with 22q11DS have disrupted dopaminergic neurotransmission, which might explain their susceptibility for psychiatric disorders.

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Simultaneous determination of catecholamines and metanephrines in urine by HPLC with fluorometric detection

Cited by 59 publications

References 12 publications

24 h Excretion Patterns of Free, Conjugated and Methylated Catecholamines in Man

24 h Excretion Patterns of Free, Conjugated and Methylated Catecholamines in Man

Specific genetic deficiencies of the A and B isoenzymes of monoamine oxidase are characterized by distinct neurochemical and clinical phenotypes.

Disrupted Dopaminergic Neurotransmission in 22q11 Deletion Syndrome

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