Simultaneous determination of captopril and hydrochlorothiazide in human plasma by reverse-phase HPLC from linear gradient elution

Huang, Taomin; He, Zhong; Yang, Bei; Shao, Luping; Zheng, Xiaowei; Duan, Gengli

doi:10.1016/j.jpba.2005.12.007

Cited by 136 publications

(70 citation statements)

References 23 publications

(21 reference statements)

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“…To measure the total captopril plasma level, two approaches were usually used. The first approach was to stabilize the SH group in the biosamples, immediately after collection, to prevent conversion of thiol compounds into the respective disulfides and/or mixed disulfides using several stabilizing agents such as N-(1-pyrenyl) maleimide (Rezende et al, 2007), N-ethylmaleimide (NEM) (Chik et al, 2014), p-bromo-phenacyl bromide (p-BPB) (Huang et al, 2006;Jankowski et al, 1995;Rezende et al, 2007;Vancea et al, 2009), monobrombimane (Kok et al, 1997;Tache et al, 2002), 2-bromo 2-acetonaphtone (Foroutan et al, 2003), o-phthaldialdehyde (Hadjmohammadi et al, 2008;Kok et al, 1997) and 1-benzyl-2-chloropyridinium bromide (Bald and Sypniewski, 1997;Bald et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…Several liquid chromatographic methods were applied for determination of captopril in plasma using UV detection, (Bald and Sypniewski, 1997;Bald et al, 1996;Ding et al, 2001;Foroutan et al, 2003;Hadjmohammadi et al, 2008;Huang et al, 2006;Jankowski et al, 1995;Li et al, 1996 ;Sultan et al, 2013;Sypniewski and Bald, 1996) fluorescence detection (Aykin et al, 2001;Kok et al, 1997;Tache et al, 2002) or mass detection (Borges et al, 2006;Chik Z et al, 2014;Du, 2007;Rezende et al, 2007;Salem et al, 2005;Vancea et al, 2009) using either protein precipitation, solid phase extraction or liquid-liquid extraction for sample preparation prior to analysis.…”

Section: Introductionmentioning

confidence: 99%

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A Liquid Chromatography/Tandem Mass Spectrometric Method for Determination of Captopril in Human Plasma: Application to a Bioequivalence Study

2017

J App Pharma Sci

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A simple, rapid and sensitive ultra-performance liquid chromatography/positive ion electrospray tandem mass spectrometric method was developed and validated for the quantification of captopril in human plasma. Following plasma protein precipitation, the analyte and internal standard Rosuvastatin were separated by ultraperformance liquid chromatography using a gradient mode mobile phase on a reversed -phase column and analyzed by mass spectrometry in the multiple reaction monitoring mode (MRM) using the respective [MRH]R ions, m/z 218.09/116.16 for captopril and m/z 482.2/ 258.17 for the internal standard. The method exhibited a linear dynamic range of 10-2000 ng/mL for captopril in human plasma. The lower limit of quantification was 10 ng/mL with a relative standard deviation of less than 6%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A total run time of 3 minutes for each sample made it possible to analyze more than 20 human plasma samples per one hour. The validated method has been successfully applied to analyze human plasma samples in a bioequivalence study of captopril after oral administration of 50 mg captopril tablet to 24 healthy subjects.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Liquid Chromatography/Tandem Mass Spectrometric Method for Determination of Captopril in Human Plasma: Application to a Bioequivalence Study

2017

J App Pharma Sci

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“…[1][2][3] Different analytical methods have been applied to the determination of the remaining drugs analyzed by us, and also by HPLC techniques. Many of the HPLC analytical methods exist for the assay of ENA, [4][5][6][7] SOT, [8][9][10][11][12][13][14][15] CAP, [16][17][18][19][20][21][22][23][24] MET, [25][26][27][28][29][30][31] 62,63 and SPI [64][65][66][67][68] in different biological fluids and tissues. HPLC methods were applied to the simultaneous quantification of selected β-blockers (also: SOT, MET and PRO) in human fluids (plasma, serum, urine).…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of an HPLC Method for the Simultaneous Analysis of 23 Selected Drugs Belonging to Different Therapeutic Groups in Human Urine Samples

2009

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We have developed and validated a new and reliable gradient reversed-phase high-performance liquid chromatography (RP-HPLC) method with a diode array detector (DAD) for the simultaneous separation and determination of 23 frequently prescribed selected drugs belonging to different therapeutic groups in human urine samples. For the drugs listed below, this method of analysis for human urine was also successfully applied to determine urine concentrations of these drugs in samples from treated patients: enalapril (ENA), paracetamol (PAR), sotalol (SOT), dipyrone (DIP), vancomycin (VAN), captopril (CAP), fluconazole (FLU), cefazolin (CEF), metoprolol (MET), aspirin (ASP), ticlopidine (TIC), prednisolone (PRE), propranolol (PRO), digoxin (DIG), sildenafil (SIL), furosemide (FUR), dexamethasone (DEX), carvedilol (CAR), ketoprofen (KET), nifedipine (NIF), terbinafine (TER), acenocoumarol (ACE) and spironolactone (SPI). Separation of the analytes was achieved by RP-HPLC-DAD with a mobile phase composed of acetonitrile, methanol and 0.05% trifluoroacetic acid in water using a gradient elution program. Good linear relationships over the investigated concentration ranges were observed with values of r 2 higher than 0.998 for all of the drugs. The intra-day and inter-day precisions of this method were evaluated with RSD values less than 4.26 and 5.42%, respectively. The relative recoveries of the 23 investigated compounds ranged from 93.60 to 106.00% with RSD values less than 4.46%. An expanded uncertainty budget was constructed for all investigated drugs in human urine samples.

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“…The structure of the components of formulation were depicted in the Figure 1. The spectrophotometric determination of atenolol in tablets 5 and simultaneous determination of captopril and hydrochlorothiazide in human plasma by reverse-phase HPLC 6 was reported in the literature. Simultaneous determination…”

Section: Introductionmentioning

confidence: 99%

RP-HPLC Method for Simultaneous Estimation of Atenolol, Hydrochlorothiazide and Losarton in Tablet Dosage form

2013

Chem Sci Trans

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Abstract:The present work describes a simple, sensitive, accurate and rapid reverse phase high performance liquid chromatographic method for simultaneous estimation and validation of atenolol, hydrochlorothiazide and losartan in tablet dosage form. Chromatography was performed on a 25 cm × 4.6 mm i.d, 5 µm particle, C18 column with use of mixture of (A) acetonitrile, methanol (65:35) and (B) 10 mM sodium dihydrogen phosphate monohydrate buffer with 0.4% v/v triethylamine and mixture of A:B (60:40 v/v) adjusted to pH 3.0 with o-phosphoric acid (5% v/v) was used as a mobile phase at a flow rate of 1.5 mL/min. The chromatograms were recorded at 230 nm. The linearity range was found 1-25 µg/mL, 1-25 µg/mL and 1-25 µg/mL for atenolol, hydrochlorothiazide and losartan respectively.

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Simultaneous determination of captopril and hydrochlorothiazide in human plasma by reverse-phase HPLC from linear gradient elution

Cited by 136 publications

References 23 publications

A Liquid Chromatography/Tandem Mass Spectrometric Method for Determination of Captopril in Human Plasma: Application to a Bioequivalence Study

A Liquid Chromatography/Tandem Mass Spectrometric Method for Determination of Captopril in Human Plasma: Application to a Bioequivalence Study

Development and Validation of an HPLC Method for the Simultaneous Analysis of 23 Selected Drugs Belonging to Different Therapeutic Groups in Human Urine Samples

RP-HPLC Method for Simultaneous Estimation of Atenolol, Hydrochlorothiazide and Losarton in Tablet Dosage form

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