Simultaneous Determination of ∊-Caprolactam and ∊-Aminocaproic Acid by Planar Chromatography

Abstract

This paper describes the qualitative and quantitative determination of E-caprolactarn and E-arninocaproic acid, which are potential contaminants of polyamid 6, by instrumental thin-layer chromatography (planar chromatography). A validation of the method is proposed. Detection was performed by photodensitornetry in UV range at 200 nm for e-caprolactarn, and at 588 nrn after derivatization by ninhydrin reagent for E-aminocaproic acid. Correlation coefficients for calibration were found about 0.996 -0.999. Repeatability results were included bet w e e n 2.4 % a nd 5.5 YO. D e r i v at i zat i o n by Over p re ss u red Derivatization (OPD) technique gave similar results as direct UV detection. 2737

Oxidation of ε-Aminocaproic Acid by Bis(dihydrogentellurato)argentite(III): A Kinetics and Mechanism Study

“…It is an antifibrinolytic agent used in the treatment and prophylaxis of haemorrhagic states associated with excessive fibrinolysis [3]. With the exception of the reference methodologies proposed by several pharmacopoeias, which are based on non-aqueous titrations, almost all of the available methodologies for aminocaproic acid determination involve either a non-specific potentiometric titration or the utilisation of time-consuming and expensive chromatographic techniques [4][5][6][7]. Aminocaproic acid is a synthetic lysine analog that suppresses fibrinolytic activity by fitting into plasminogen's lysinebinding site and preventing the binding of plasminogen to fibrin.…”