Simultaneous determination of benzodiazepines by ultraviolet-visible spectrophotometry in micellar media

Abstract

A method for the simultaneous determination of benzodiazepines in binary mixtures is proposed, based on the acid hydrolysis of benzodiazepines to benzophenones and the spectrophotometric determination of the latter in the presence of Nemol K 1030, a non-ionic surfactant condensate of ethylene oxide with nonylphenol. The experimental conditions for the hydrolysis of several benzodiazepines in sealed Pyrex tubes were determined. The addition of Nemol K 1030 to acidic solutions of benzophenones modified the positions of the absorption bands and made possible the simultaneous analysis of binary mixtures of benzodiazepines.

Screening for Drugs of Abuse (II): Cannabinoids, Lysergic Acid Diethylamide, Buprenorphine, Methadone, Barbiturates, Benzodiazepines and other Drugs

“…The EC response is, however, highly temperature-dependent being IOfi times greater at 100uC than at 350°C. 222 GC characteristics for 19 benzophenones were reported by Schutz and Westenbergerv'' and UV spectra by de la Guardia et al 224 This method allowed simultaneous estimation of different benzophenones. It should be noted that some of the newer benzodiazepines, such as alprazolam and triazolam, do not form benzophenones.…”