Simultaneous determination of bentazone and its metabolites in postmortem whole blood using liquid chromatography–tandem mass spectrometry

Cho, Byungsuk; Kim, Suncheun; In, Sangwhan; Choe, Sanggil

doi:10.1016/j.forsciint.2017.07.024

Cited by 11 publications

(1 citation statement)

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“…To test if the probes can also inhibit plant P450s, we monitored inhibition of maize (Zea mays) ZmCYP81A9, for which activity assays have been established, because it is an important herbicide-metabolizing enzyme responsible for the modification of herbicides with unrelated modes of action [15][16][17]. ZmCYP81A9 was expressed in brewer's yeast (Saccharomyces cerevisiae, [18]) and microsomes from this strain were isolated and used to monitor the conversion of bentazone, leading to hydroxybentazone (Figure 2a), which can be measured by LC-MS [19]. ZmCYP81A9 activity can be inhibited with the broad-range P450 inhibitor 1aminobenzotriazole (1-ABT, [20]), which was included as a positive control for inhibition.…”

Section: Zmcyp81a9 Is Inhibited By P450 Probesmentioning

confidence: 99%

Discovery of active mouse, plant and fungal cytochrome P450s in endogenous proteomes and upon expressionin planta

Farre,

Brown,

Toth

et al. 2024

Preprint

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Eukaryotes produce a large number of cytochrome P450s that mediate the synthesis and degradation of diverse endogenous and exogenous metabolites. Yet, most of these P450s are uncharacterized and global tools to study these challenging, membrane-resident enzymes remain to be exploited. Here, we applied activity profiling of plant, mouse and fungal P450s with chemical probes that become reactive when oxidized by P450 enzymes. Identification by mass spectrometry revealed labeling of a wide range of active P450s, including six plant P450s, 40 mouse P450s and 13 P450s of the fungal wheat pathogen Zymoseptoria tritici. We next used transient expression of GFP-tagged P450s by agroinfiltration to show ER-targeting and NADPH-dependent, activity-based labeling of plant, mouse and fungal P450s. Both global profiling and transient expression can be used to detect a broad range of active P450s to study e.g. their regulation and discover selective inhibitors.

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