Simultaneous determination of atorvastatin, metformin and glimepiride in human plasma by LC–MS/MS and its application to a human pharmacokinetic study

Polagani, Srinivasa Rao; Pilli, Nageswara Rao; Gajula, Ramakrishna; Gandu, Venkateswarlu

doi:10.1016/j.jpha.2012.09.002

Cited by 60 publications

(35 citation statements)

References 45 publications

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“…Both APCI (Swales et al, 2010) and ESI (Liu & Coleman, 2009;Chen et al, 2011;Polagani et al, 2013;Al Bratt et al, 2017) ionization methods have been employed, with the latter being largely preferred even if ion suppression phenomena can arise. Despite these potential limitations, most methods permitted to obtain an easy multi-drug PK analysis in low plasma sample volumes ( 0.2 mL) after a simple protein precipitation procedure.…”

Section: B Glucose-lowering Agents and Their Metabolismmentioning

confidence: 99%

“…Alternative sample preparation procedures were the liquid-liquid extraction (LLE), the solid phase extraction (SPE), and DBS extraction (Shin et al, 2011;Scherf-Clavel & H€ ogger, 2015;Shah et al, 2016). Metformin PK has been assessed in combination with several other glucose-lowering agents including: rosiglitazone (Zhang et al, 2007;Chen et al, 2011) glimepiride (Gu et al, 2010;Shin et al, 2011;Polagani et al, 2013), and several dipeptidyl-peptidase-4 inhibitors, (Pontarolo et al, 2014;Shah et al, 2016;Al Bratt et al, 2017) with no or minimal analytical interference.…”

Section: B Glucose-lowering Agents and Their Metabolismmentioning

confidence: 99%

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The role of mass spectrometry in studies of glycation processes and diabetes management

DaRonco

Crotti

Agostini

et al. 2018

Mass Spectrometry Reviews

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In the last decade, mass spectrometry has been widely employed in the study of diabetes. This was mainly due to the development of new, highly sensitive, and specific methods representing powerful tools to go deep into the biochemical and pathogenetic processes typical of the disease. The aim of this review is to give a panorama of the scientifically valid results obtained in this contest. The recent studies on glycation processes, in particular those devoted to the mechanism of production and to the reactivity of advanced glycation end products (AGEs, AGE peptides, glyoxal, methylglyoxal, dicarbonyl compounds) allowed to obtain a different view on short and long term complications of diabetes. These results have been employed in the research of effective markers and mass spectrometry represented a precious tool allowing the monitoring of diabetic nephropathy, cardiovascular complications, and gestational diabetes. The same approaches have been employed to monitor the non‐insulinic diabetes pharmacological treatments, as well as in the discovery and characterization of antidiabetic agents from natural products. © 2018 Wiley Periodicals, Inc. Mass Spec Rev 38:112–146, 2019.

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Section: B Glucose-lowering Agents and Their Metabolismmentioning

confidence: 99%

Section: B Glucose-lowering Agents and Their Metabolismmentioning

confidence: 99%

The role of mass spectrometry in studies of glycation processes and diabetes management

DaRonco

Crotti

Agostini

et al. 2018

Mass Spectrometry Reviews

View full text Add to dashboard Cite

show abstract

“…LC-MS study was carried out using human plasma for the determination of metformin, atorvastatin, and glimepiride [8]. The extraction was done with protein precipitation with acetonitrile.…”

Section: Review Articlementioning

confidence: 99%

Assessment of Chromatographic Separations for Antihypertensive and Antidiabetic Drugs

Venkataraman

2018

Asian J Pharm Clin Res

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Context: The focus of this review is to compile the different chromatographic methods that were reported earlier for the analysis of different antihypertensive and antidiabetic drugs. Objective: The magnitude of chemical entities investigated and entering into the medicinal field for various health-related ailments is escalating year after year. The drugs are either innovative entities or fractional structural variation of the preexisting chemical molecule. These drugs may exhibit unexpected toxicities after Phase IV of clinical trials, resulting in their withdrawal from the market. Under these circumstances, analytical measures for these drugs may not be accessible in the pharmacopeias. The main aim of this work is to compile the different analytical techniques for the quantification of various antihypertensive drugs and antidiabetic drugs. Methods: The present work is to thoroughly study the literature for the application of different analytical techniques such as high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectroscopy/tandem mass spectroscopy (LC-MS/MS) for the quantification of antihypertensive drugs and anti-diabetic drugs. Results: The present study attempts to collate various analytical techniques that were developed and validated for the estimation of few important antidiabetic and antihypertensive drugs either in pure, individually or combined with other pharmaceutical dosage form by HPLC, LC-MS/MS, and high-performance thin-layer chromatography techniques. Conclusion: Different chromatographic methods are considered to be rapid tools for qualitative and quantitative analysis of newer chemical entities in pharmaceuticals. The amount of these newer chemical entities which are reaching the pharmaceutical market is increasing day by day nevertheless there exists a lag in establishing the standard protocols for the identification, impurity profiling, related substance and assay method. Hence, the present review compiles the different analytical methods that were reported in the literature and thus helps the researchers and chemists to make use of the analytical techniques for the quantification and validation of various antidiabetic and antihypertensive drugs.

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“…9,10 INVO-KAMET TM (Janssen Pharmaceuticals, Inc.) a combination of Canagliflozin and Metformin is available in the market with variable dosing. Many analytical methods were reported in literature for the determination of Canagliflozin 5,11 and Metformin [12][13][14][15][16][17][18][19][20][21][22] individually or with some other drugs in biological samples. These methods are only suitable for quantitation of one drug in biological samples selectively and sensitively may not be suitable for simultaneous quantification Canagliflozin and Metformin.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Canagliflozin and Metformin in Human Plasma by LC–MS/MS Assay and its Application to a Human Pharmacokinetic Study

Ramisetti¹,

Atmakuri²,

Venkata³

et al. 2019

IJPER

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Objective:The main objective of this work was to develop a simple, rapid and sensitive liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the simultaneous quantification of Canagliflozin and Metformin. Methods: Deuterated compounds of respective drugs were used an internal standard. Sample extraction was carried out using a simple Protein Precipitation (PP) technique. A C 18 column with an isocratic mobile phase composed of 5mM ammonium acetate with 0.01% formic acid and methanol were used for chromatographic separation. Results: The method was validated in the linearity range of 10.00-6028.00 ng/mL for Canagliflozin and 10.00-3027.00 ng/mL for Metformin. The precision and accuracy results over five concentration levels in five different batches were well within the acceptance limits. A variety of stability tests were executed in plasma and in neat samples are comply with the FDA guidelines. Conclusion: The proposed assay method is simple, rapid and sensitive for the simultaneous determination of Canagliflozin and Metformin in human plasma. This method was successfully used to quantitate the in vivo plasma concentrations obtained from a pharmacokinetic study.

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Simultaneous determination of atorvastatin, metformin and glimepiride in human plasma by LC–MS/MS and its application to a human pharmacokinetic study

Cited by 60 publications

References 45 publications

The role of mass spectrometry in studies of glycation processes and diabetes management

The role of mass spectrometry in studies of glycation processes and diabetes management

Assessment of Chromatographic Separations for Antihypertensive and Antidiabetic Drugs

Simultaneous Determination of Canagliflozin and Metformin in Human Plasma by LC–MS/MS Assay and its Application to a Human Pharmacokinetic Study

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