Simultaneous Determination of Atorvastatin and Amlodipine in Industrial Tablets by Apparent Content Curve and HPLC Methods

The development of two eco-friendly analytical methods for the simultaneous determination of eight cardiovascular drugs; hydrochlorothiazide (HCT), captopril (CPL), lisinopril (LSP), valsartan (VAL), atorvastatin (ATR), bisoprolol (BSL), amlodipine (AML) and carvedilol (CVL); alongside with the nutraceutical vincamine (VIC) is essential for sustainable pharmaceutical analysis. This study explores the application of Micellar Electro Kinetic Chromatography (MEKC) and High-Performance Liquid Chromatography (HPLC) for this purpose. In MEKC method, the separation was done using fused silica capillary (41.5 cm × 50 µm id) and a back ground electrolyte consisting of 50 mM borate buffer (pH 9) containing 50 mM sodium lauryl sulphate (SLS) and 10% organic modifier (Acetonitrile). In HPLC method, separation was performed on a ZORBAX Extend-C18 (4.6 × 250 mm, 5 µm) column, using a gradient mobile phase consisting of 50 mM phosphate buffer pH 3 and methanol. Both methods attained good linearity (r ≥ 0.9996) with low values of LOD and LOQ. Both methods were successfully applied in the determination of co-administered single, binary and ternary dosage form of the studied drugs. Moreover, application of various combinations of co-administered dosage forms was achieved in rat plasma, confirming the applicability of these methods in different matrices. The use of micellar solutions in MEKC enhances separation efficiency while reducing the need for organic solvents, aligning with green chemistry principles. HPLC methods were optimized using environmentally benign solvents, ensuring reduced toxicity and waste production. The methodologies were evaluated through green, white, and blue metrics to ensure comprehensive sustainability, considering ecological impact, safety, and practical efficiency. These methods were not only cost-effective and time-saving but achieved high efficiency, sensitivity, and reproducibility making them ideal for routine use in pharmaceutical analysis.

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Simple spectrophotometric methods for the determination of amlodipine and atorvastatin in bulk and tablets

Reid

Farid

Eltayeb

2021

Futur J Pharm Sci

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Background Simultaneous spectrophotometric determination of samples containing more than one analyte presents analytical challenge; the choice of an analytical procedure is strictly related to the extent of overlapping between the individual absorption peaks of these components; if the absorption peaks are satisfactorily resolved, the determination is not problematic, but if the individual component signals are partly or totally overlapped, then powerful techniques are needed. Combined amlodipine and atorvastatin are typical example where special techniques are needed to resolve bands overlapping. Results Application of multiwavelength regression and absorbance factor methods to the analysis of atorvastatin and amlodipine combination proved to be satisfactorily capable of accurate and precise determination of the two analytes. The two methods recoveries were very close to the expected analytes concentrations, and the precision of the methods was < 2% relative standard deviation. Statistical comparison indicated that there is no significant difference between the assay results obtained by the two method as the calculated t values 0.91 and 1.13 for amlodipine and atorvastatin, respectively, were less than the tabulated t value 2.23 at 95% confidence level. Conclusion The proposed methods are accurate, precise, simple and inexpensive. They can be applied successfully to the analysis of the two drugs in combined dosage form.

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Simultaneous Determination of Atorvastatin and Amlodipine in Industrial Tablets by Apparent Content Curve and HPLC Methods

Cited by 4 publications

References 8 publications

A New method for simultaneous qualitative and quantitative determination of amlodipine besylate and atorvastatin calcium in bulk and pharmaceutical formulations using transmission FT-IR spectroscopy

A New method for simultaneous qualitative and quantitative determination of amlodipine besylate and atorvastatin calcium in bulk and pharmaceutical formulations using transmission FT-IR spectroscopy

Simple simultaneous analysis of various cardiovascular drug mixtures with vincamine: comparative eco-friendly assessment

Simple spectrophotometric methods for the determination of amlodipine and atorvastatin in bulk and tablets

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