Simultaneous Determination of Atenolol and Nifedipine by Using Spectrophotometric Method with Multivariate Calibration and HPLC Method Implementing ?Design of Experiment?

Abstract

Objectives: The aim of the present work is to develop rapid and simple methods for the simultaneous determination of atenolol and nifedipine. Multivariate calibration using preprocessing methods to enhance results in case of presence of any interference in samples and RP-HPLC method for achieving a good separation with accepted system suitability parameters with the use of design of experiment for robustness testing according to Plackett-Burman design. Methods: The spectrophotometric method is based on the measurement of the mixture in the range of 200-400 nm then applies the multivariate calibration methods for resolution of the binary mixture mainly using partial least squares (PLS) and principal component regression (PCR). The proposed RP-HPLC method utilizes an YMC-pack pro C 18 column (250 mm x 4.6 mm, 5 μm). Optimum chromatographic conditions were attained by application of Design of experiment (DOE). Both spectrophotometric and chromatographic methods were applied on the determination of both drugs in marketed capsules. Also, dissolution testing of these capsules was investigated. Results: The recovery percentage for nifedipine and atenolol in capsules dosage form were found to be in PLS method (100.50 ± 0.850, 100.78 ± 1.07), PCR method (100.60 ± 0.960, 100.72 ± 1.09) and RP-HPLC method (99.77 ± 0.560, 100.90 ± 1.23); respectively. Conclusion: The methods were validated according to ICH guidelines. All the results obtained were found to be within the acceptable limits. The methods were successful to estimate nifedipine and atenolol in bulk powder and pharmaceutical preparation.