Simultaneous determination of ascorbic acid and dehydroascorbic acid in fish tissues by high-performance liquid chromatography

Ito, Takafumi; Murata, Hisashi; Yasui, Yoshihide; Matsui, Morimasa; Sakai, Tadashi; Yamauchi, Kiyoshi

doi:10.1016/0378-4347(95)00030-m

Cited by 16 publications

(6 citation statements)

References 4 publications

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“…The diets were analysed for proximate composition, total carotenoids and vitamins A, C and E as described previously (Watanabe, Wada, Takeuchi & Arai 1981; Watanabe 1988; Schierle, Hardi, Faccin, Buhler & Schuep 1995; Ito, Murata, Yasui, Matsui, Sakai & Yamauchi 1995).…”

Section: Methodsmentioning

confidence: 99%

Influence of various dietary synthetic carotenoids on bio-defence mechanisms in rainbow trout,Oncorhynchus mykiss(Walbaum)

Amar

Kiron

Satoh

et al. 2001

Aquaculture Research

157

112

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This study examined the in¯uence of different carotenoids on growth and some immune indices in rainbow trout. Six semipuri®ed casein-based diets were formulated to contain one of three different carotenoids: astaxanthin, canthaxanthin and b-carotene, at 100 mg kg ±1 , each of them with vitamins A, C and E either added or omitted. The two control diets contained no carotenoids and were either with or without the vitamins. Rainbow trout weighing about 140 g were fed the diets for 9 weeks. Speci®c growth rate, feed:gain ratio and nonspeci®c immune parameters were determined. Growth and feed conversion were similar among the groups. Immune parameters like production of reactive oxygen species by head kidney leukocytes and plasma total immunoglobulin levels did not vary with the treatment. Serum complement activity in both b-carotene groups and the vitamincontaining astaxanthin group were signi®cantly higher than both the control ®sh. Serum lysozyme activity in the vitamin-containing bcarotene and astaxanthin groups were signi®cantly different from both control groups. Phagocytic activity was also high in the vitamin-containing b-carotene and astaxanthin groups compared with the controls. For phagocytic index, in addition to the foregoing groups, the vitamin-containing canthaxanthin group gave better results compared with the controls. The vitamin-containing astaxanthin and b-carotene groups also exhibited better nonspeci®c cytotoxicity for the peripheral blood lymphocytes at all effector-to-target ratios. Thus, among the carotenoids studied, b-carotene and astaxanthin elevated humoral factors such as serum complement and lysozyme activity, as well as cellular factors such as phagocytosis and nonspeci®c cytotoxicity. In the presence of the vitamins the carotenoids exerted a greater in¯uence on the bio-defense mechanisms of rainbow trout.

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Section: Methodsmentioning

confidence: 99%

Influence of various dietary synthetic carotenoids on bio-defence mechanisms in rainbow trout,Oncorhynchus mykiss(Walbaum)

Amar

Kiron

Satoh

et al. 2001

Aquaculture Research

157

112

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show abstract

“…Studies have shown that animals synthesize AsA in particular tissues (Drouin et al, 2011; Ito et al, 1995; Travica et al, 2017). Lower vertebrates, such as fish, amphibians, and reptiles, synthesize VC only in their kidneys (Dabrowski et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Bombyx mori can synthesize ascorbic acid through the l‐gulose pathway to varying degrees depending on developmental stage

Hou

Dai

Liang

et al. 2021

Arch Insect Biochem Physiol

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Vitamin C (VC) is an essential nutrient for many animals. However, whether insects, including Bombyx mori, can synthesize VC remains unclear. In this article, the optimized HPLC method was used to determine the content of l‐ascorbic acid (AsA) in silkworm eggs, larvae and pupae, and the activity of l‐gulono‐1,4‐lactone oxidase (GULO), a key enzyme in VC synthesis. The RNA interference method was used to determine the effect of the BmGulo‐like gene on embryonic development and GULO activity in the pupal fat body. The AsA content increased significantly during E144 h–E168 h in the late embryonic stage and P48 h–P144 h in the middle‐late pupal stage, in which exogenous VC was not ingested. Furthermore, the body AsA content in larvae fed VC‐free feed also increased with larval stage. The GULO enzymatic activity was present in eggs and the fat bodies of larvae and pupae, even when the larvae were reared with fresh mulberry leaves. Moreover, the activity was higher in the later embryonic stages (E144 h–E168 h) and the early pupal stage (before P24 h). The GULO activity in the pupal fat body dramatically decreased when the screened BmGulo‐like gene (BGIBMGA005735) was knocked down with small interfering RNA; in addition, the survival rate and hatching rate of eggs significantly decreased 21% and 44%, respectively, and embryonic development was delayed. Thus, Bombyx mori can synthesize AsA through the l‐gulose pathway, albeit with low activity, and this synthesis ability varies with developmental stages.

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“…Vitamin C ---Vitamin C (ascorbic acid) concentration in diets and liver homogenates was determined by post-column HPLC (PC-HPLC) methods. 8,9) A mixture of 100 µl of 5-fold diluted homogenates of samples and 100 µl 5% metaphosphoric acid was stored at -80°C until the analysis of Vitamin C. Then, these samples were mixed with 200 µl of 5% metaphosphoric acid and centrifuged at 12000 × g at 4°C for 5 min. 50 µl of the supernatants obtained was subjected to the PC-HPLC.…”

Section: Methodsmentioning

confidence: 99%

Feeding Period of Selenium-Deficient Diet and Response of Redox Relating Minerals

Matsumoto

Ariyoshi

Terada

et al. 2006

JOURNAL OF HEALTH SCIENCE

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Simultaneous determination of ascorbic acid and dehydroascorbic acid in fish tissues by high-performance liquid chromatography

Cited by 16 publications

References 4 publications

Influence of various dietary synthetic carotenoids on bio-defence mechanisms in rainbow trout,Oncorhynchus mykiss(Walbaum)

Influence of various dietary synthetic carotenoids on bio-defence mechanisms in rainbow trout,Oncorhynchus mykiss(Walbaum)

Bombyx mori can synthesize ascorbic acid through the l‐gulose pathway to varying degrees depending on developmental stage

Feeding Period of Selenium-Deficient Diet and Response of Redox Relating Minerals

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