Simultaneous determination of ascorbic acid and caffeine in commercial soft drinks using reversed-phase ultraperformance liquid chromatography

Abstract

A new reversed-phase ultraperformance liquid chromatography method with a photodiode array detector was developed for the quantification of ascorbic acid (AA) and caffeine (CAF) in 11 different commercial drinks consisting of one energy drink and 10 ice tea drinks. Separation of the analyzed AA and CAF with an internal standard, caffeic acid, was performed on a Waters BEH C column (100 mm × 2.1 mm, 1.7 μm i.d.), using a mobile phase consisting of acetonitrile and 0.2M HPO (11:89, v/v) with a flow rate of 0.25 mL/min and an injection volume of 1.0 μL. Calibration graphs for AA and CAF were computed from the peak area ratio of AA/internal standard and CAF/internal standard detected at 244.0 nm and 273.6 nm, respectively. The developed reversed-phase ultraperformance liquid chromatography method was validated by analyzing standard addition samples. The proposed reversed-phase ultraperformance liquid chromatography method gave us successful results for the quantitative analysis of commercial drinks containing AA and CAF substances.

Development of a method for simultaneous analysis of caffeine and taurine in energy drinks by micellar electrokinetic chromatography with diode-array detector

“…Caffeine is considered one of the most common ingredients to any brand of energy drink (Paula Lima & Farah, 2019;McLellan et al, 2016;Rai et al, 2016;Turak et al, 2017). On the human body, it has a stimulation effect both in the central nervous system and the cardiac activities (Paula Lima & Farah, 2019;McLellan et al, 2016;Seifert et al, 2011).…”

“…On the human body, it has a stimulation effect both in the central nervous system and the cardiac activities (Paula Lima & Farah, 2019;McLellan et al, 2016;Seifert et al, 2011). However, when ingested at doses above 400 mg/day (considering a 70 kg adult) it may cause anxiety and other undesirable symptoms (McLellan et al, 2016;Reid et al, 2016;Temple, 2019;Tran et al, 2016;Turak et al, 2017). From 1.000 mg/day, it presents toxicity to the organism, and it may be lethal in quantities of 5.000 to 10.000 mg/day (Seifert et al, 2011).…”

Capillary electrophoresis method for simultaneous analysis of caffeine, vanillin and ethyl vanillin in beverages

“…Caffeine (Figure 1A), chemically 1,3,5-trimethylxanthine, is a naturally occurring alkaloid and it is existed in different plant species and is present in their seeds, leaves, and fruits [1]. Caffeine is major components to the most type of soft drink, liquid dietary supplements and beverages [2][3][4][5]. Caffeine commonly is added to soft drinks as flavoring agent Article Related Abbreviations: BGE, background electrolyte; EOF, electroosmotic flow; IS, internal standard; PTFE, polytetrafluoroethylene; RSD, relative standard deviation and is intentionally added to consumers attracted to these drinks.…”

Determination of Caffeine Content in Commercial Energy Beverages Available in Saudi Arabian Market by Gas Chromatography-Mass Spectrometric Analysis

“…ese techniques include UV spectrophotometry [19], HPLC [15,20], capillary electrophoresis [19], RP-UPLC [21], gas chromatography [22], GC-MS [1,23,24], continuous flow analyzer coupled with UV-Vis and evaporative light scattering detectors [17], solid phase-FT-Raman spectroscopy [25], and microemulsion electrokinetic chromatography [26].…”