Simultaneous determination of artemisinin and its analogs and flavonoids in <i>Artemisia annua</i> crude extracts with the use of NMR spectroscopy

Kontogianni, Vassiliki G.; Primikyri, Alexandra; Sakka, Marianna; Gerothanassis, Ioannis P.

doi:10.1002/mrc.4971

Cited by 20 publications

(14 citation statements)

References 35 publications

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“…Thus, based on the results of this study, we preliminarily speculated that AAE could reduce the content and gene expression level of IL-1β and IL-6 in the intestinal mucosa of broilers by regulating the TLR4/NF-κB signaling pathway. The reason might be that A. annua aqueous extract contains bioactive components (polysaccharides, polyphenols, flavonoids) that play an immunoregulatory role (18,27), and previous studies showed that polysaccharides in Artemisia could regulate the immune function of broilers through the TLR4/NF-κB signaling pathway (39). However, the specific mechanism of dietary AAE regulating intestinal immune function of broilers needs further study.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, artemisinin, an antimalarial component of A. annua studied by Tu, has attracted wide attention worldwide (12). A. annua has multiple properties, such as anticancer (13), anti-malarial (14), anti-inflammatory (15), and antioxidant (11), which are related to its rich bioactive constituents, including polysaccharides (16), polyphenols (17), and flavonoids (18). It was reported that dietary inclusion of dried A. annua leaves was used for the coccidiosis treatment and growth advancement in broilers (19,20).…”

Section: Introductionmentioning

confidence: 99%

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Artemisia annua L. Aqueous Extract Promotes Intestine Immunity and Antioxidant Function in Broilers

Guo

Xing

et al. 2022

Front. Vet. Sci.

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This study was conducted to investigate the effects of Artemisia annua L. aqueous extract (AAE) on intestinal immune and antioxidative function of broilers. A total of 200 one-day-old Arbor Acre broilers were randomly allotted into five dietary treatment groups, with five replicates per treatment and eight broilers per replicate. The five treatment diets were formulated by adding, respectively, 0 (control group), 0.5, 1.0, 1.5, and 2.0 g/kg AAE in the basal diet. The results showed that dietary inclusion of AAE quadratically decreased interleukin (IL)-1β content, linearly decreased IL-6 content in the small intestine through regulating the nuclear factor-kappa B signal pathway, and quadratically increased immunoglobulin (Ig)M and sIgA content in ileum and jejunum. Besides, there was a quadratic decrease in the gene expression of IL-1β, IL-6, and toll like receptor 4 (TLR4) in ileum on day 21, and the gene expression of IL-6 and TLR4 in duodenum on day 42, thereby improving small intestinal immune function in broilers. Additionally, dietary inclusion of AAE improves antioxidative function through the nuclear factor-erythroid 2-related factor 2 (Nrf2) signal pathway in the small intestinal mucosa of broilers, especially, quadratically increased catalase (CAT) and superoxidase dismutase activity in ileum, and total antioxidant capacity and glutathione peroxidase activity in duodenum, and quadratically decreased malondialdehyde concentration in ileum, besides, linearly increased heme oxygenase-1 and Nrf2 gene expression in jejunum and ileum on day 42, quadratically increased CAT gene expression in the small intestine. Furthermore, regression analyses of the above parameters showed that the optimal dose range of AAE in the diet of broilers was 1.12–1.38 g/kg.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Artemisia annua L. Aqueous Extract Promotes Intestine Immunity and Antioxidant Function in Broilers

Guo

Xing

et al. 2022

Front. Vet. Sci.

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“…Compounds 2 and 5 had never been reported before from plants of the genus Achillea. The remaining eight compounds (Figure 2) were phenolic derivatives, identified as chlorogenic acid (10) [19], the polymethoxylated flavonols penduletin (11) [20] and artemetin (12) [21], the polymethoxylated flavones santoflavone (13) [22] and salvigenin ( 14) [23] and the flavone-C-glycosides vitexin (15) [24], isovitexin ( 16) [25], and isoschaftoside (17) [26]. Wilhelmsin (7) was isolated as an optically active colorless amorphous solid with molecular formula C19H25NO6, as determined by HR-ESIMS.…”

Section: Chemical Analysis and Structure Elucidation Of Wilhelmsin And Wilhelmsolidementioning

confidence: 99%

Salvigenin, a Trimethoxylated Flavone from Achillea Wilhelmsii C. Koch, Exerts Combined Lipid-Lowering and Mitochondrial Stimulatory Effects

et al. 2021

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Phytochemical analysis of the Iranian plant Achillea wilhelmsii led to the isolation of 17 pure secondary metabolites belonging to the classes of sesquiterpenoids and phenolics. Two of these compounds, named wilhemsin (7) and wilhelmsolide (9), are new sesquiterpenoids, and the first shows undescribed structural features. Their structures were elucidated through extensive spectroscopic analysis, mainly based on 1D and 2D NMR, and chemical derivatization. Starting from plant traditional use and previous reports on the activity of the plant extracts, all the pure compounds were evaluated on endpoints related to the treatment of metabolic syndrome. The sesquiterpene hanphyllin (8) showed a selective cholesterol-lowering activity (−12.7% at 30 µM), santoflavone (13) stimulated glucose uptake via the GLUT transporter (+16.2% at 30 µM), while the trimethoxylated flavone salvigenin (14) showed a dual activity in decreasing lipid levels (−22.5% palmitic acid biosynthesis at 30 µM) and stimulating mitochondrial functionality (+15.4% at 30 µM). This study further confirms that, in addition to the antioxidants vitexin, isovitexin, and isoschaftoside, A. wilhelmsii extracts contain molecules that can act at different levels on the metabolic syndrome symptoms.

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“…The existing methods for the characterization of flavonoids include: wave spectrometry [ 23 ], UV spectrometry [ 24 ], thin-layer chromatography [ 25 ], High-Performance Liquid Chromatography (HPLC) [ 26 ], Ultra-Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS) [ 27 ], UPLC-MS combined with database method [ 28 ], HPLC-MS combined with mass spectrometry cleavage law method, etc. [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Extract identification and evaluation of the cytotoxic activity of Polygala fallax Hemsl in Heilongjiang ethnic medicine against tumors

Yang

Lang

2023

THC

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BACKGROUND: Heilongjiang Province is a frontier province with distinctive characteristics, fertile land and rich products. OBJECTIVE: This study provides a new method for qualitatively studying flavonoids in traditional Chinese medicine and a new auxiliary means for identifying flavonoid isomers. METHODS: The flavonoids in Polygala fallax Hemsl were identified by ultra-performance liquid chromatography-photo-diode array (PDA)-quadrupole-electro- static field orbitrap mass spectrometry tandem by UV Spectrum, primary and secondary high-resolution mass spectrometry (MS1/MS2) cleavage of fragments combined with databases, mass spectrometry cleavage patterns and literature. RESULTS: The established QSRR model was used to verify the flavonoids identified from the Polygala fallax Hemsl. CONCLUSION: The structure of multiple Polygala fallax Hemsl has been identified using various spectral methods. The tumor cytotoxic activity of the isolated compounds was evaluated. This paper is of great significance for further elucidating the pharmacodynamic substance basis and further developing and utilizing Polygala fallax Hemsl.

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Simultaneous determination of artemisinin and its analogs and flavonoids in Artemisia annua crude extracts with the use of NMR spectroscopy

Cited by 20 publications

References 35 publications

Artemisia annua L. Aqueous Extract Promotes Intestine Immunity and Antioxidant Function in Broilers

Artemisia annua L. Aqueous Extract Promotes Intestine Immunity and Antioxidant Function in Broilers

Salvigenin, a Trimethoxylated Flavone from Achillea Wilhelmsii C. Koch, Exerts Combined Lipid-Lowering and Mitochondrial Stimulatory Effects

Extract identification and evaluation of the cytotoxic activity of Polygala fallax Hemsl in Heilongjiang ethnic medicine against tumors

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