Simultaneous determination of anthelmintic drugs by capillary electrophoresis using cyclodextrins as buffer additives

Abstract

A generic capillary zone electrophoresis with diode array detection was developed for the simultaneous determination of five anthelmintic drugs (albendazole, fenbendazole, flubendazole, mebendazole, levamisole) used in human and veterinary medicine. The separation of structurally related benzimidazole derivatives was resolved by addition in the background electrolyte of randomly methylated β-cyclodextrin (RAMEB). The optimization of the analytical conditions was carried out using a "one factor at a time" tehnique. Optimized conditions were found to be 50 mM phosphate buffer at pH 1.9, 5 mM RAMEB as buffer additive, applied voltage + 25 kV, system temperature 20 °C, injection parameters 50 mbar/1 s. Good analytical performance with regards to linearity, reproducibility, accuracy and robustness was achieved. The method was applied for the determination of analytes from pharmaceutical preparations.

Applications of capillary electrophoresis in the fields of environmental, pharmaceutical, clinical, and food analysis (2019–2021)

“…Five anthelmintic drugs (albendazole, fenbendazole, furbendazole, mebendazole, and levamisole) belong to benzimidazole derivatives and have similar structures, which makes them impossible to be analyzed simultaneously with the same method. However, Hancu et al [38] have made use of the CZE with diode array detection (DAD) method for simultaneous determination of anthelmintic drugs by adding randomly methylated βcyclodextrin to the BGE. The addition of randomly methylated β-cyclodextrin in the BGE improved resolution and enhanced the selectivity.…”

“…However, Hancu et al. [38] have made use of the CZE with diode array detection (DAD) method for simultaneous determination of anthelmintic drugs by adding randomly methylated β ‐cyclodextrin to the BGE. The addition of randomly methylated β ‐cyclodextrin in the BGE improved resolution and enhanced the selectivity.…”

Chemical and pharmacological characterization of anthelmintic benzimidazoles

“…A kapilláris elektroforézis is több esetben alkalmazott módszer [25][26]. Hatásmechanizmus Az AHBi hatásmechanizmusa azon alapszik, hogy a tubulin fehérjéhez kötődve gátolják annak polimerizációját, így a citoplazma fontos alkotóelemének, a mikrotubulusoknak a képződést.…”