Simultaneous determination of androstenedione and testosterone in human serum by liquid chromatography-tandem mass spectrometry

Abstract

We have developed a sensitive and precise LC-MS/MS method, which gives more accurate results for all androstenedione measurements and low testosterone concentrations than immunoassay.

Age-associated changes in hypothalamic–pituitary–testicular function in middle-aged and older men are modified by weight change and lifestyle factors: longitudinal results from the European Male Ageing Study

“…A further strength is consideration of the feedback-feed-forward regulatory nature of the HPT axis by examining multiple hormones, enabling differentiation between hypothalamic and testicular malfunctions. In our previous cross-sectional study, a gas chromatographymass spectrometry method was used for measuring testosterone (1), which showed a high concordance correlation (RZ0.95, P!0.001; data not shown) with the validated LC-MS/MS method (25) used in this analysis. In the present longitudinal study, parallel analyses of both baseline and follow-up samples, together in the same LC-MS/MS run (25), not only ensured optimal accuracy and precision (40) but also minimised inter-laboratory and methodological variations.…”

“…In our previous cross-sectional study, a gas chromatographymass spectrometry method was used for measuring testosterone (1), which showed a high concordance correlation (RZ0.95, P!0.001; data not shown) with the validated LC-MS/MS method (25) used in this analysis. In the present longitudinal study, parallel analyses of both baseline and follow-up samples, together in the same LC-MS/MS run (25), not only ensured optimal accuracy and precision (40) but also minimised inter-laboratory and methodological variations. The ability to use data from such a sizeable general population cohort in a real-world setting is another one of the study's major strengths.…”

“…Testosterone was measured by liquid chromatographytandem mass spectrometry (LC-MS/MS) (25), with paired baseline and follow-up samples analysed simultaneously. LH and SHBG were measured by the E170 platform electrochemiluminescence immunoassay (Roche Diagnostics).…”

“…Free testosterone (FT) was calculated from testosterone, SHBG and albumin, using the Vermeulen formula (26). Intra-and inter-assay coefficients of variation were as follows: testosterone 4.0 and 5.6%; SHBG 1.7 and 3.2% and LH 1.9 and 3.0% respectively (1,25). Stability of samples during long-term storage was established with no evidence of any systematic drift with time.…”

Automated and sensitive determination of four anabolic androgenic steroids in urine by online turbulent flow solid-phase extraction coupled with liquid chromatography–tandem mass spectrometry: A novel approach for clinical monitoring and doping control

“…Furthermore, AAS are even misused by athletes to improve their sports performance [6,15]. Although the use of AAS has been banned in sports and governed by the World Anti-Doping Agency since 1976, some of the priority controlled substances such as methenolone were still detected in urine at the [19,20] and LC-MS/MS [21][22][23]. Immunoassay is a feasible method in the clinical laboratory but it is susceptible to matrix interference especially for determination of low concentration.…”