Simultaneous Determination of Amlodipine Besylate and Atorvastatin Calcium in Binary Mixture by Spectrofluorimetry and HPLC Coupled with Fluorescence Detection

Abstract

Caduet tablets are novel prescription drug that combines amlodipine besylate (AM) with atorvastatin calcium (AT). A spectrofluorimetric and an HPLC-fluorescence detection methods were developed for simultaneous determination of both drugs in tablets. In the spectrofluorimetric method, native fluorescence of AM and AT were measured in methanol at 442 and 369 nm upon excitation at 361 and 274 nm, respectively. The emission spectrum of each drug reveals zero value at the emission wavelength of the other drug, thus allowing their simultaneous determination without interference. In the HPLC method, separation of AM and AT was achieved within 8 minutes on a C18 column using acetonitrile:phosphate buffer (0.015 M, pH 3) (45:55, v/v) as the mobile phase. Fluorescence detection was carried out using excitation wavelengths 361 and 274 nm and emission wavelengths 442 and 378 nm for AM and AT, respectively. Excellent linearity was observed. Careful validation proved advantages of the new methods: high sensitivity, accuracy, selectivity and suitability for quality control laboratories.

Quantitative Determination of Amlodipine Besylate, Losartan Potassium, Valsartan and Atorvastatin Calcium by HPLC in their Pharmaceutical Formulations

“…BP described a RP-HPLC method for assay of Amlodipine besylate and potentiometric titrations for assay of Atorvastatin calcium, Losartan potassium and Valsartan [3]. Several methods have been published for simultaneous determination of studied drugs in their combinations with each other, these methods depends on different analytical technique like Spectrophotometry [4][5][6][7][8][9][10][11], Spectrofluorimetry [12][13][14], Capillary Electrophoresis [15][16][17][18], HPTLC and TLC [19][20][21] and HPLC coupled with UV detector [21][22][23][24][25][26][27][28][29], fluorescence detector [30] and mass spectrometer detector [31][32][33][34]. Our scope is development of a validated analytical method for assay of these drugs in combination with each other in its pharmaceutical preparations and it should be characterized by a simplicity, accuracy, preciseness and sensitivity.…”

Quantification of amlodipine and atorvastatin in human plasma by UPLC–MS/MS method and its application to a bioequivalence study

“…Several analytical methods have been described for the simultaneous determination of AML and ATO in pharmaceutical formulations. These methods include spectroscopy (Darwish, Hassan, Salem, & El‐Zeany, ; Khan & Jain, ; Ramesh & Ramakrishna, ; Sohrabi, Abdolmaleki, & Dehroudi, ), HPLC with UV detection (Mohammadi et al, ; Rajeswari, Sankar, Rao, & Seshagirirao, ; Sivakumar, Manavalan, Muralidharan, & Valliappan, ) and HPLC with fluorescence detection (Moussa, El‐Zaher, Mahrouse, & Ahmed, ). Simultaneous determination of AML and ATO in human plasma was done by HPLC–MS/MS methods (Pilli et al, ; Yacoub, Awwad, Alawi, & Arafat, ; Zhou et al, ), but these methods show several limitations.…”

Quantitative Determination of Amlodipine Besylate, Losartan Potassium, Valsartan and Atorvastatin Calcium by HPLC in their Pharmaceutical Formulations

“…BP described a RP-HPLC method for assay of Amlodipine besylate and potentiometric titrations for assay of Atorvastatin calcium, Losartan potassium and Valsartan [3]. Several methods have been published for simultaneous determination of studied drugs in their combinations with each other, these methods depends on different analytical technique like Spectrophotometry [4][5][6][7][8][9][10][11], Spectrofluorimetry [12][13][14], Capillary Electrophoresis [15][16][17][18], HPTLC and TLC [19][20][21] and HPLC coupled with UV detector [21][22][23][24][25][26][27][28][29], fluorescence detector [30] and mass spectrometer detector [31][32][33][34]. Our scope is development of a validated analytical method for assay of these drugs in combination with each other in its pharmaceutical preparations and it should be characterized by a simplicity, accuracy, preciseness and sensitivity.…”