Simultaneous determination of amitriptyline, imipramine and perphenazine in pharmaceutical preparations and blood serum by multi-wavelength spectrophotometry

Development of an HPLC method for the monitoring of tricyclic antidepressants in biofluids

“…The vast majority of the published methods involve liquid -liquid extraction (LLE) with different extraction media: n-hexane [7,10,13,25,27], mixtures of hexane with isoamyl alcohol in different ratios (98:2 or 99:1) [9,12,17,32], mixture of n-heptane, ethylacetate and isoamyl alcohol [8], mixture of isoamyl alcohol and heptane [30], and mixture of heptane and ethyl acetate [34], or diethyl ether [22]. SPE was applied to the isolation of TCAs from biomatrices using various sorbents C 18 [6,14,19,29,33] cyanopropyl cartridges using ASPEC XL automatic SPE apparatus by Gilson with high recoveries (93 -98%) [15], on-line extraction on an Oasism HLB extraction column (30 lm, 1650 mm) [24] and 3M-Empore highperformance extraction disc cartridges [17].…”

“…Other techniques used for the analysis of TCAs in biofluids include voltammetry with lipid-coated electrodes [26], derivative spectrometry using both first-and secondderivative spectra [27], fluorescence polarisation immunoassay [28], GC [29], GC-MS [30], nonaqueous CE [31 -33], CE with electrochemiluminescence detection based on end-column reaction of tris-(2,2-bipyridyl)ruthenium (II) with aliphatic tertiary amino moieties [34].…”

A validated spectrofluorimetric method for determination of some psychoactive drugs

“…These methods include; spectrophotometry [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18], spectrofluorimetry [19][20][21][22][23][24], titrimetry [25][26][27][28][29][30], chromatography [31][32][33][34][35][36][37][38][39][40][41][42], flow injection analysis [43][44][45][46][47], radio-immunoassay [48][49], electrochemical methods [50][51] and chemiluminescence methods [52]…”

Application of two chemometric methods for the determination of imipramine, amitriptyline and perphenazine in content uniformity and drug dissolution studies

“…Among the various analytical techniques, UV spectrophotometric method remains one of the most popular methods for quantitive analysis of binary or ternary mixtures of tricyclic antidepressants combined with neuroleptics drugs [12][13][14]. UV-vis spectra usually contain non-specific data, which can be converted into useful information by derivative or multivariate calibration methods [15][16][17][18][19].…”