Simultaneous determination of adenosine and its metabolites by capillary electrophoresis as a rapid monitoring tool for 5′-nucleotidase activity

Tzeng, Huey-Fen; Hung, Chao-Hsien; Wang, Jeng-Yu; Chou, Chih-Hong; Hung, Hsueh-Ping

doi:10.1016/j.chroma.2006.08.030

Cited by 16 publications

(3 citation statements)

References 30 publications

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“…Finally, additional assay protocols utilize capillary electrophoresis (CE) coupled with UV detection (Table , entry e) − or high performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS) (Table , entry f). − In the CE assay method, the products of the CD73 enzymatic reaction are separated based on different electrophoretic mobility within fused-silica capillaries exposed to a high voltage (15 kV). − Following the enzymatic reaction, which can be performed directly in a capillary, the products are then quantified by UV absorption at 260 nm. In the nanoscale CE protocol originally reported for rat CD73, the LOD for adenosine was ∼1 μM, the limit of quantitation (LOQ) was ∼4 μM, and AMPCP had a K i value of 0.87 ± 0.02 μM (rat CD73), which is 4-fold higher than the K i determined using the radiometric TLC assay (AMPCP K i = 197 ± 5 nM, rat CD73) .…”

Section: Small-molecule Cd73 Inhibitorsmentioning

confidence: 99%

Targeting Metabolism of Extracellular Nucleotides via Inhibition of Ectonucleotidases CD73 and CD39

Jeffrey¹,

Lawson²,

Powers³

2020

J. Med. Chem.

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In the tumor microenvironment, unusually high concentrations of extracellular adenosine promote tumor proliferation through various immunosuppressive mechanisms. Blocking adenosine production by inhibiting nucleotidemetabolizing enzymes, such as ectonucleotidases CD73 and CD39, represents a promising therapeutic strategy that may synergize with other immuno-oncology mechanisms and chemotherapies. Emerging small-molecule ectonucleotidase inhibitors have recently entered clinical trials. This Perspective will outline challenges, strategies, and recent advancements in targeting this class with smallmolecule inhibitors, including AB680, the first small-molecule CD73 inhibitor to enter clinical development. Specific case studies, including structure-based drug design and lead optimization, will be outlined. Preclinical data on these molecules and their ability to enhance antitumor immunity will be discussed.

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Section: Small-molecule Cd73 Inhibitorsmentioning

confidence: 99%

Targeting Metabolism of Extracellular Nucleotides via Inhibition of Ectonucleotidases CD73 and CD39

Jeffrey¹,

Lawson²,

Powers³

2020

J. Med. Chem.

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“…The analysis of the above indicated nitrogenous compounds in various matrices and using various procedures has been reported in the literature. Adenosine, for example, was analyzed using high-performance liquid chromatography HPLC (9) or capillary electrophoresis (10). Analysis of deoxyfructosazines (DFs) was less frequently reported in the peer reviewed literature (11,12).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Certain Nitrogenous Compounds in Tobacco. Part 1: Adenosine, 2,5- and 2,6-Deoxyfructosazines, Mannosamine and Glucosamine

Moldoveanu

Byrd

Gerardi

2011

Beiträge Zur Tabakforschung International/Contributions to Tobacco Research

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Nitrogenous compounds such as amino acids and proteins are frequently analyzed in tobacco since they are considered precursors of toxicants in cigarette smoke. However, much less attention is given to other nitrogenous compounds such as amino sugars and deoxyfructosazines, although their concentration in tobacco can be equal to or even higher than that of most free amino acids. These nitrogenous compounds may contribute to the formation of toxicants in smoke, or may contribute to the sensory properties of cigarette smoke, reasons for which their analysis is important. This study describes a procedure for the analysis of adenosine, 2,5-and 2,6-deoxyfructosazines (DFs), mannosamine and glucosamine in tobacco. The analysis uses a liquid chromatography-tandem mass spectrometry (LC/MS/MS) technique. Sample preparation for analysis consists of the extraction of the tobacco with a solution of 90% water and 10% methanol, followed by filtration. The separation of the analytes was done on a hydrophilic interaction liquid chromatography HILIC column using an isocratic procedure with a solvent consisting of 78% CH 3 CN, 22% H 2 O, that also contained 0.1 % HCOOH and 0.143 g/L CH 3 COONH 4 . The measurements were done using electrospray positive ionization mass spectrometric detection. The analytical procedure was validated and was proven very reliable. A number of tobaccos were analyzed, including several fluecured and Burley USA tobaccos, off-shore tobaccos, two Oriental tobaccos, two green tobaccos, as well as tobaccos from commercial and Kentucky reference cigarettes. The ranges for the analytes per g tobacco were found between 0.4 and 20.3 µg/g for adenosine, between 0.0 and 608.5 µg/g for 2,5-DF, between 0.0 and 424.5 µg/g for 2,6-DF, between 12.5 and 415.5 µg/g for mannosamine and between 25.9 and 1885.7 µg/g for glucosamine.The study also indicated that the levels of DFs and that of the amino sugars in tobacco show a very good correlation. This correlation can be explained by the same source of the two classes of compounds, namely the reaction of (reducing) sugars and ammonia. [Beitr. Tabakforsch. Int. 24 (2011) 233-242] ZUSAMMENFASSUNG Stickstoffhaltige Verbindungen im Tabak, wie Aminosäuren und Proteine, werden häufig untersucht, da sie als Vorläufer für toxische Substanzen im Zigarettenrauch betrachtet werden. Dagegen wird anderen stickstoffhaltigen Verbindungen wie Aminozuckern und Deoxyfructosazinen weniger Aufmerksamkeit gewidmet, obwohl deren Konzentration im Tabak genauso groß oder sogar größer ist, als die von freien Aminosäuren. Dass diese stickstoffhaltigen Verbindungen zu der Bildung von toxischen Substanzen im Tabakrauch oder zu den sensorischen Eigenschaften von Zigarettenrauch beitragen können, sind wichtige Gründe für die Untersuchung. Diese Arbeit beschreibt ein Verfahren zur Analyse von Adenosin, 2,5-und 2,6-Deoxyfructosazinen (DFs), Mannosamin und Glucosamin im Tabak. Zur Analyse wurde eine liquid chromatography-tandem mass spectrometry (LC/MS/MS)-Technik verwendet. Die Probenvorbereitung für die ...

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“…A great quantity of adenosine is released into body uid/blood when the oxygen-supply balance is disturbed. 3 Conventional analytical methods, such as radioimmunoassay (RIA), 4,5 high-performance liquid chromatography (HPLC) [6][7][8] and capillary electrophoresis (CE), 9,10 have been successfully used to detect adenosine. But these techniques/methods normally require time-consuming sample pretreatment processes, expensive instrumentation and well-trained technicians, all of which limit their application.…”

Section: Introductionmentioning

confidence: 99%

Exonuclease III assisted aptasensor for adenosine detection with gold nanoparticle probes

2014

Anal. Methods

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Simultaneous determination of adenosine and its metabolites by capillary electrophoresis as a rapid monitoring tool for 5′-nucleotidase activity

Cited by 16 publications

References 30 publications

Targeting Metabolism of Extracellular Nucleotides via Inhibition of Ectonucleotidases CD73 and CD39

Targeting Metabolism of Extracellular Nucleotides via Inhibition of Ectonucleotidases CD73 and CD39

Analysis of Certain Nitrogenous Compounds in Tobacco. Part 1: Adenosine, 2,5- and 2,6-Deoxyfructosazines, Mannosamine and Glucosamine

Exonuclease III assisted aptasensor for adenosine detection with gold nanoparticle probes

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