Simultaneous Determination of Abscisic Acid and Its Catabolites by Hydrophilic Solid-Phase Extraction Combined with Ultra High Performance Liquid Chromatography–Tandem Mass Spectrometry

Abstract

An efficient and selective pretreatment method of one-step hydrophilic interaction chromatography-based solid phase extraction (HILIC SPE) was developed using silica as the sorbent to quickly and sensitively detect endogenous ABA and its five catabolites in fresh Oryza sativa tissues. The extracted analytes were sensitively quantified with ultra high performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS). Under the optimized conditions, good linearity of the developed analytical method was obtained in the range of 0.2–1000 ng/mL with linear correlation coefficients (r) greater than 0.9987. The limits of detection (LODs, signal/noise = 3) ranged from 0.01 to 0.74 ng/mL. The relative recoveries were between 83.3% and 112.0% with the relative standard deviations (RSDs) ranging from 0.5 to 15.0%. Using the proposed method, the concentration variations of ABA and its catabolites were monitored in the salt-stressed rice tissues.

The phytomelatonin receptor PMTR1 regulates seed development and germination by modulating abscisic acid homeostasis in Arabidopsis thaliana

“…The extraction and determination of ABA, phaseic acid (PA), and dihydrophaseic acid (DPA) were performed by following the protocol described in our previous reports. 41,44 Briefly, [ 2 H 6 ] ABA (Olomouc), [ 2 H 3 ] PA (National Research Council Plant Biotechnology Institute), and [ 2 H 3 ] DPA (National Research Council Plant Biotechnology Institute) were used as internal standards. Plant tissues of 50 mg were frozen in liquid nitrogen and ground into fine powder.…”

Analysis of anticancer compound, indole-3-carbinol, in broccoli using a new ultrasound-assisted dispersive-filter extraction method based on poly(deep eutectic solvent)-graphene oxide nanocomposite

“…Several extraction methods such as SPE, dispersive solid-phase extraction (DSPE), and matrix solid-phase dispersion (MSPD) have been developed [ 18 , 19 ]. SPE affords high recovery and remarkable reproducibility, but the adsorbent amount required is large (tens to hundreds of milligrams), and it often requires an activation step (pretreatment with methanol and water) before loading the sample solution [ 20 , 21 ]. In DSPE, the adsorbent is uniformly dispersed to ensure sufficient contact with the sample solution, washing solvent, and elution solvent, such that adsorption and desorption occur rapidly [ 22 , 23 ].…”

Determination of anthraquinone in tea by stable isotope dilution assay-gas chromatography-tandem mass spectrometry

“…Among many techniques, solid-phase extraction (SPE) is considered one of the most effective techniques for coextraction due to its convenience, robustness, and rapidity. Therefore, SPE was used here for the analysis of tea samples [14,15].…”