2010
DOI: 10.1016/j.jchromb.2010.08.017
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Simultaneous determination of 8-hydroxy-2′-deoxyguanosine and 5-methyl-2′-deoxycytidine in DNA sample by high performance liquid chromatography/positive electrospray ionization tandem mass spectrometry

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Cited by 19 publications
(9 citation statements)
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References 34 publications
(37 reference statements)
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“…It can be found after the fragmentation, when the main [M+H] + adduct of 5mdC appears at m/z 126.0662. This ion originates due to the cleavage of the N -glycoside bond and transfer of a hydrogen atom from the sugar molecule [24]. Because these findings are comparable to the detection of dC and dG, the precursor/product ion pairs of m/z 228.0979/112.0505 for dC, m/z 242.1135/126.0662 for 5mdC and m/z 268.1040/152.0780 for dG were used as MRM transitions.…”
Section: Resultsmentioning
confidence: 99%
“…It can be found after the fragmentation, when the main [M+H] + adduct of 5mdC appears at m/z 126.0662. This ion originates due to the cleavage of the N -glycoside bond and transfer of a hydrogen atom from the sugar molecule [24]. Because these findings are comparable to the detection of dC and dG, the precursor/product ion pairs of m/z 228.0979/112.0505 for dC, m/z 242.1135/126.0662 for 5mdC and m/z 268.1040/152.0780 for dG were used as MRM transitions.…”
Section: Resultsmentioning
confidence: 99%
“…There might therefore be a connection between genetic and epigenetic alterations, possibly via oxidative stress in such patients. Several reports have investigated the relationship between oxidized DNA and DNA methylation [5558], including the simultaneous examination of 8-OHdG and 5MedC in DNA samples [55]. 8-OHdG may induce hypomethylation of DNA by inhibiting DNA methylation at nearby cytosine bases [58].…”
Section: Discussionmentioning
confidence: 99%
“…Therefore, the 8-OHdG levels are considered to be a valid marker of oxidative DNA damage. In addition, new techniques have been introduced into this field as gas chromatography with mass spectrometry [17,25], electrospray ionization mass spectrometry (MS) [26], liquid chromatography with mass spectrometry [27][28][29][30] and capillary electrophoresis [31][32][33][34][35][36]. From the direct ones, high performance liquid chromatography (HPLC) with electrochemical detection (ED) [23,24] belongs to the most commonly used method for determination of 8-OHdG.…”
Section: Determination Of 8-ohdgmentioning
confidence: 99%