Simultaneous determination of 6β-hydroxycortisol and cortisol in human urine by liquid chromatography with ultraviolet absorbance detection for phenotyping the CYP3A activity determined by the cortisol 6β-hydroxylation clearance

Furuta, Takahisa; Mori, Chisato; Suzuki, Atsushi; Shibasaki, Hiromi; Yokokawa, Akitomo; Kasuya, Yasuji

doi:10.1016/j.jchromb.2003.11.001

Cited by 26 publications

(28 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Plasma concentrations of cortisol were analyzed by gas chromatography-mass spectrometry using cortisol-13 C 4 as the internal standard (Furuta et al, 2000a). Urinary excreted amounts of 6b-OHF were analyzed by high-performance liquid chromatography using 6a-hydroxycorticosterone as the internal standard (Furuta et al, 2004;Shibasaki et al, 2012). Both the interassay and intra-assay coefficients of variation (CV) were ,1.80% for gas chromatography-mass spectrometry-selected ion monitoring, and both were ,2.5% for high-performance liquid chromatography.…”

Section: Chemicalsmentioning

confidence: 99%

Intraindividual and Interindividual Variabilities in Endogenous Cortisol 6β-Hydroxylation Clearance as an Index for In Vivo CYP3A Phenotyping in Humans

et al. 2012

Self Cite

View full text Add to dashboard Cite

This study was designed to evaluate the in vivo activity of cytochrome P450 3A (CYP3A) in 49 healthy Japanese subjects aged 22-58 years using endogenous cortisol 6b-hydroxylation clearance [CLm(6b)], a novel biomarker for CYP3A phenotyping. CLm(6b) in the 49 healthy subjects was 2.40 6 0.79 ml/min with an approximately 4-fold interindividual variability of CYP3A activity. The mean clearance in the 24 women was 2.50 6 0.89 ml/min; the value in the women was higher than in the 25 men (2.30 6 0.69 ml/min) by approximately 9%. We also measured the change of CLm(6b) in 14 healthy subjects in the morning at 10:00-12:00 every 2 or 3 days over a period of 36-53 days and observed a 1.5-fold to 3.4-fold dayto-day intraindividual variability in the CYP3A activity. The mean value for CLm(6b) in each subject for 36-53 days was 2.54 6 0.76 ml/min (n = 14). We also evaluated the CLm(6b) every 2 hours from 8:00-20:00 in 26 healthy subjects. The within-day intraindividual clearance variability was 1.1-fold to 2.5-fold (2.45 6 0.91 ml/min, n = 26). No characteristic diurnal rhythms were observed in the in vivo activity of CYP3A.

show abstract

Section: Chemicalsmentioning

confidence: 99%

Intraindividual and Interindividual Variabilities in Endogenous Cortisol 6β-Hydroxylation Clearance as an Index for In Vivo CYP3A Phenotyping in Humans

et al. 2012

Self Cite

View full text Add to dashboard Cite

show abstract

“…Here we describe a simple, cost-effective and rapid method for simultaneous determination of F, E and 6b-OHF in human urine with adequate sensitivity using UPLC-UV detection. By taking advantage of UPLC techniques on smaller particle size (1.7 lm) and higher operating pressures, this method can offer efficient chromatography for the simultaneous determination of F, E, and 6b-OHF in human urine with reduced run time (7 min) compared to traditional HPLC method with a long analysis time of about 27 min [8,13].…”

Section: Clinical Applicationmentioning

confidence: 99%

“…The levels of urinary F, E, and 6b-OHF have been determined by various techniques such as enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA), but these techniques have relatively low selectivity and are time consuming and have largely been replaced by high performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC/MS) [8], and ultra high performance liquid chromatography tandem mass spectrometric (UPLC/MS/MS) [9]. Simultaneous determination of F, E, and 6b-OHF has the advantage of evaluating the activities of both renal 11bHSD2 and CYP3A.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Cortisol, Cortisone, and 6β-Hydroxycortisol in Human Urine by UPLC with UV Detector and Application to Determine Diurnal Variations

Xiao

Chan

et al. 2011

Chromatographia

View full text Add to dashboard Cite

In the present studies, a simple rapid ultra performance liquid chromatography (UPLC) method with UV detection for the simultaneous determination of cortisol, cortisone and 6b-hydroxycortisol in human urine was developed. The three analytes and the internal standard dexamethasone were separated on a Waters Acquity UPLC-Tunable UV system with an Acquity UPLC BEH C18 column (50 9 2.1 mm ID, 1.7 lm) using a gradient elution of methanol and water (containing 0.01% formic acid) with a run time of 7 min. The method was accurate and precise, over the ranges of 5-200 ng mL -1 for cortisol, and 10-1,000 ng mL -1 for both cortisone and 6b-hydroxycortisol, and showed good linearity (r 2 [ 0.999). This method was applied for the measurement of cortisol, cortisone and 6b-hydroxycortisol in samples collected over different periods as a tool to assess the activity of CYP3A and 11b-hydroxysteroid dehydrogenase type 2 enzymes.

show abstract

“…Various methods for the simultaneous determination of 6␤-OHF and F in urine have been described, such as highperformance liquid chromatographic (HPLC) with UV [6][7][8] and fluorescence detection [9], gas chromatography-mass spectrometry (GC-MS) [10], and liquid chromatography-tandem mass spectrometry (LC-MS-MS) [11]. Separate detection of 6␤-OHF and F has been performed by radioimmunoassay (RIA) [12], enzyme-linked immunosorbentassay (ELISA) [13].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of 6β-hydroxycortisol and cortisol in human urine and plasma by liquid chromatography with ultraviolet absorbance detection for phenotyping the CYP3A activity

Gong

et al. 2005

Journal of Chromatography B

View full text Add to dashboard Cite

Simultaneous determination of 6β-hydroxycortisol and cortisol in human urine by liquid chromatography with ultraviolet absorbance detection for phenotyping the CYP3A activity determined by the cortisol 6β-hydroxylation clearance

Cited by 26 publications

References 41 publications

Intraindividual and Interindividual Variabilities in Endogenous Cortisol 6β-Hydroxylation Clearance as an Index for In Vivo CYP3A Phenotyping in Humans

Intraindividual and Interindividual Variabilities in Endogenous Cortisol 6β-Hydroxylation Clearance as an Index for In Vivo CYP3A Phenotyping in Humans

Simultaneous Determination of Cortisol, Cortisone, and 6β-Hydroxycortisol in Human Urine by UPLC with UV Detector and Application to Determine Diurnal Variations

Simultaneous determination of 6β-hydroxycortisol and cortisol in human urine and plasma by liquid chromatography with ultraviolet absorbance detection for phenotyping the CYP3A activity

Contact Info

Product

Resources

About