Simultaneous determination of 2-deoxy-D-glucose and D-glucose in rat serum by high-performance liquid chromatography with post-column fluorescence derivatization.

Umegae, Yoshihiko; Nohta, Hitoshi; Ohkura, Yosuke

doi:10.1248/cpb.38.963

Cited by 6 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A range of derivatizing agents has been developed for this purpose p-hydroxybenzoic hydrazide [16,17], 2-cyanoacetamide [18], benzamidine [19,20], meso-1,2-bis(4-methoxyphenyl) ethylenediamine [21], 4-methoxybenzamidine [22], ethanolamine [23]. We chose benzamidine as a derivatization agent because of its high selectivity, reactivity, sensitivity and convenience [21, 24 30].…”

Section: Post-column Derivatizationmentioning

confidence: 99%

Rapid screening of airway secretions for fucose by parallel ligand-exchange chromatography with post-column derivatization and fluorescence detection

et al. 2001

View full text Add to dashboard Cite

Key WardsColumn liquid chromatography Ligand-exchange chromatography Post-col u m n derivatization Fluorescence detection Fucose in airway secretions SummaryFucose (6-deoxygalactose) is a constituent of airway mucous glycoproteins. In this paper we describe a high-throughput method for screening nasal lavage fluid samples and induced sputum samples for fucose. Fucose was released by hydrolysis with 0.5 M sulfuric acid at 100 ~ for4 h. After pH adjustment remaining proteins were removed by on-line dialysis. Chromatography was performed with I',,vo 300 mm x 7.8 mm i.d. Bio-Rad Aminex HPX-87H columns arranged in a box-car configuration. Post-column derivatization was performed with benzamidine under alkaline conditions. Fluorescence was monitored at an excitation wavelength of 360 nm, using an optical cut-off filter of 420 nm. The limit of quantitation for fucose was 40 gM (S/N = 3) in 300 gL nasal lavage mediu m, with use of a 20-gL injection loop. Relative standard deviation (RSD) values for intra and inter assay data were below 15% and 20%, respectively, at spike levels of 635 gM I_-fucose. The method was used to monitor the fucose content of human airway secretions.

show abstract

Section: Post-column Derivatizationmentioning

confidence: 99%

Rapid screening of airway secretions for fucose by parallel ligand-exchange chromatography with post-column derivatization and fluorescence detection

et al. 2001

View full text Add to dashboard Cite

show abstract

“…For 2‐DG quantitation in biological samples, Umegae et al . reported a method for post‐column fluorescent derivatization by reaction with meso ‐1, 2‐ bis (4‐methoxyphenyl)ethylene‐diamine in an alkaline medium after separation on a strong anion exchange column (Umegae et al ., ). Other methods for analyzing the presence of 3 H‐2‐DG in rat muscle using ion exchange chromatography have been reported (Wallis et al ., ); however, the method is only useful for radiolabeled 2‐DG.…”

Section: Introductionmentioning

confidence: 97%

“…Thus, HPLC‐UV is not the method of choice when the analytes are to be quantitated in submicrogram quantities in biologics. Therefore, highly sensitive and reproducible pre‐column and post‐column fluorescence derivatization methods were developed for the quantitation of mono and oligosaccharides in biologics (Anumula, ; Kakita et al ., ; Umegae et al ., ). For 2‐DG quantitation in biological samples, Umegae et al .…”

Section: Introductionmentioning

confidence: 99%

“…Thus, HPLC-UV is not the method of choice when the analytes are to be quantitated in submicrogram quantities in biologics. Therefore, highly sensitive and reproducible pre-column and post-column fluorescence derivatization methods were developed for the quantitation of mono and oligosaccharides in biologics (Anumula, 1994;Kakita et al, 2002;Umegae et al, 1990). For 2-DG quantitation in biological samples, Umegae et al reported a method for post-column fluorescent derivatization by reaction with meso-1, 2-bis(4-methoxyphenyl)ethylenediamine in an alkaline medium after separation on a strong anion exchange column (Umegae et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, highly sensitive and reproducible pre-column and post-column fluorescence derivatization methods were developed for the quantitation of mono and oligosaccharides in biologics (Anumula, 1994;Kakita et al, 2002;Umegae et al, 1990). For 2-DG quantitation in biological samples, Umegae et al reported a method for post-column fluorescent derivatization by reaction with meso-1, 2-bis(4-methoxyphenyl)ethylenediamine in an alkaline medium after separation on a strong anion exchange column (Umegae et al, 1990). Other methods for analyzing the presence of 3 H-2-DG in rat muscle using ion exchange chromatography have been reported (Wallis et al, 2002); however, the method is only useful for radiolabeled 2-DG.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A validated bioanalytical HPLC method for pharmacokinetic evaluation of 2‐deoxyglucose in human plasma

Gounder¹,

Lin²,

Stein³

et al. 2011

Biomedical Chromatography

View full text Add to dashboard Cite

2-deoxyglucose (2-DG), an analog of glucose, is widely used to interfere with the glycolysis in tumor cells and studied as a therapeutic approach in clinical trials. To evaluate the pharmacokinetics of 2-DG, we describe the development and validation of a sensitive HPLC fluorescent method for the quantitation of 2-DG in plasma. Plasma samples were deproteinized with methanol and the supernatant was dried at 45°C. The residues were dissolved in methanolic sodium acetate/boric acid solution. 2-DG and other monosaccharides were derivatized to 2-aminobenzoic acid derivatives in a single step in the presence of sodium cyanoborohydride at 80°C for 45min. The analytes were separated on a YMC ODS C18 reversed-phase column using gradient elution. The excitation and emission wavelengths were set at 360 and 425nm. The 2-DG calibration curves were linear over the range of 0.63 to 300μg/mL with the limit of detection of 0.5μg/mL. The assay provided satisfactory intra-day and inter-day precision with RSD less than 9.8% and the accuracy ranged from 86.8% to 110.0%. The HPLC method is reproducible and suitable for the quantitation of 2-DG in plasma. The method was successfully applied to characterize the pharmacokinetics profile of 2-DG in patients with advanced solid tumors.

show abstract

Fluorogenic reactions for biomedical chromatography

Ohkura

Kai

Nohta

1994

Journal of Chromatography B: Biomedical Sciences and Applicatio

View full text Add to dashboard Cite

Simultaneous determination of 2-deoxy-D-glucose and D-glucose in rat serum by high-performance liquid chromatography with post-column fluorescence derivatization.

Cited by 6 publications

References 0 publications

Rapid screening of airway secretions for fucose by parallel ligand-exchange chromatography with post-column derivatization and fluorescence detection

Rapid screening of airway secretions for fucose by parallel ligand-exchange chromatography with post-column derivatization and fluorescence detection

A validated bioanalytical HPLC method for pharmacokinetic evaluation of 2‐deoxyglucose in human plasma

Fluorogenic reactions for biomedical chromatography

Contact Info

Product

Resources

About