Simultaneous determination of 18 tetrahydrocorticosteroid sulfates in human urine by liquid chromatography/electrospray ionization-tandem mass spectrometry

Quantitative Assessment of Dietary (Poly)phenol Intake: A High-Throughput Targeted Metabolomics Method for Blood and Urine Samples

“…However, it is a common misconception that recoveries lower than 50% are unacceptable, and given the good results obtained for precision (intra- and interday) and accuracy, results on ME and recovery did not compromise quantification. Low recoveries of some highly hydrophilic compounds such as sulfates have been previously reported, likely due to their low capacity of retention in HLB sorbents. ,, Higher efficiencies for sulfates in urine using other alternatives such as anion exchange sorbents OASIS MAX or WAX have been reported due to their specificity for fully ionized anions. , However, SPE extraction with HLB has recently been shown to be more efficient than MAX sorbent overall for the analysis of a wide range of (poly)­phenol metabolites, as in a present work . Additionally, recoveries obtained in the present study were precise (CV < 15%) (Tables and ), which also supports the use of HLB sorbent for this application.…”

“…29,33,35 Higher efficiencies for sulfates in urine using other alternatives such as anion exchange sorbents OASIS MAX or WAX have been reported due to their specificity for fully ionized anions. 35,36 However, SPE extraction with HLB has recently been shown to be more efficient than MAX sorbent overall for the analysis of a wide range of (poly)phenol metabolites, as in a present work. 33 Additionally, recoveries obtained in the present study were precise (CV < 15%) (Tables 2 and 3), which also supports the use of HLB sorbent for this application.…”