Simultaneous determination of 18 abused opioids and metabolites in human hair using LC–MS/MS and illegal opioids abuse proven by hair analysis

Simultaneous determination of LSD and 2-oxo-3-hydroxy LSD in hair and urine by LC–MS/MS and its application to forensic cases

“…These methods are not as sensitive as those employing LC-MS, and large amounts of sample are required for analysis (25-112 mg). In the present study, LSD and O-H-LSD in hair were extracted by a pre- viously published LC-MS/MS method that has been used for routine analysis of drugs of abuse in our laboratory [21,[27][28][29]. Methanol extraction employed in this study has been applied to the extraction of most drugs including hydrolysis-susceptible and lipophilic drugs [30].…”

Maternal and Neonatal Hair and Breast Milk in The Assessment of Perinatal Exposure to Drugs of Abuse

“…Due to the small amount of hair specimen usually available for analysis, multianalyte methods are recommended, saving time, costs and amount of specimen required. To date, several analytical procedures for testing of drugs in hair have been reported, with most of them, focused on the validation of methods for the detection of drugs of abuse usually found in hair [60][61][62][63][64][65][66][67][68][69][70][71][72][73][74][75][76][77][78][79]. The most important validation parameters (recovery, intra-assay and interassay precision and accuracy and limit of detection and quantification) as a function of analytical method and matrix extraction for different drugs of abuse determined in hair are summarized in Table 1.…”

“…To test for the high number of drugs and metabolites in hair samples, pharmacotoxicology laboratories are required to update analytical procedures, in order to achieve rapid, simple and sensitive analyses with reduced sample preparation and instrumental processing, to finally increase overall sample-throughput. Different methods have been proposed for testing class-specific groups of compounds [66,69,[72][73][74][77][78][79], but only a few articles describe extensive analysis of multiclass drugs [60,61,[63][64][65]67,68,75,76,80] with some including also methods for THC quantification [62,70,71].…”

“…Currently, triple quadrupole type mass spectrometers are the most widely used for LC-MS testing and have become the preferred analytical instruments for drugs of abuse detection [65,[69][70][71][73][74][75][76][77][78]. Triple quadrupoles can perform single quadrupole experiments such as full scan and selected ion monitoring (SIM) experiments and have the capability of specialized tandem (MS/MS) scan modes such as product ion scanning, precursor ion scanning, constant neutral loss and multiple reaction monitoring.…”

Cocaine contamination in pubic hair: Analysis of the decontamination method

“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] Nevertheless, the methods used for hair testing are limited by the risk of false positives due to external contamination. [17][18][19][20][21] The search for a method that can discriminate between passive exposure and active drug consumption has long been debated.…”