Simultaneous determination of 15 marker constituents in various Radix Astragali preparations by solid‐phase extraction and high‐performance liquid chromatography

Abstract

An improved quality control method was developed to simultaneously determine 15 major constituents (eight flavonoids and seven saponins) in various radix Astragali preparations, using SPE for pretreatment of samples, HPLC with diode-array and evaporative light scattering detectors (DAD-ELSD) for quantification in one run, and HPLC-ESI-TOF/MS for definite identification of compounds in preparations. Optimum separations were obtained with a ZORBAX C(18) column, using a gradient elution with 0.3% aqueous formic acid and ACN. This established method was fully validated with respect to linearity, precision, repeatability, and accuracy, and was successfully applied to quantify the 15 compounds in 19 commercial samples, including 3 dosage forms, i. e., oral solution, injection, concentrated granule, and its processed products of radix Astragali. The results demonstrated that many factors might result in significant differences in quality of the final preparations, including crude drugs, pretreatment processes, manufacturing procedure, storage conditions, etc. Then the developed method provided a reasonable and powerful manner to ensure the efficacy, safety, and batch-to-batch uniformity of radix Astragali products by standardizing each procedure, and thus should be proposed as quality control for the clinical use and modernization of herbal preparations.

Characterization of novel astragaloside malonates from Radix Astragali by HPLC with ESI quadrupole TOF MS

“…Astragalosides (triterpene saponins in Radix Astragali), with evident bioactivities of protection of cardiovascular system [9][10][11], anti-tumor [6], hepatoprotective [12] and neuroprotective [13], have been commonly used as ''marker constituents'' for quality control of the crude herbal medicine and related preparations [1,14,15]. The qualitative and quantitative analysis of astragalosides mainly focused on astragaloside I, II, IV and their isomers [1,[14][15][16].…”

Determination and quantification of astragalosides in Radix Astragali and its medicinal products using LC–MS

“…Astragalosides have been analyzed by thin-layer chromatography (TLC) [18], HPLC coupled with an ultraviolet absorption detector (HPLC -UV) [17,19], HPLC coupled with ultraviolet and evaporative light scattering detectors (HPLC -UV -ELSD) [20,21], and HPLC coupled with diode array and evaporative light scattering detectors (HPLC -DAD -ELSD) [10,22,23]. However, HPLC -UV suffers from unsatisfactory detection limits because astragalosides possess very few chromophore groups and can only be detected at short wavelengths (200 -210 nm).…”

Chromatographic Fingerprint Analysis and Characterization of Constituents in Shenqi Fuzheng Injection by HPLC-DAD-ELSD and UFLC-DAD-Q-TOF Tandem Mass Spectrometry Techniques

“…The other components were tentatively identified according to their MS fragmentation pattern, and also by studying their UV spectral characteristics and reported literatures. Accordingly, the peaks were ascribed to cytidine (1) [27,28], uracil (2) [29,30], guanosine (4) [31,32], adenine nucleoside (6) [17,18], syringoside (7) [33,34] (10) [35], isomucronulatol-7,2′-di-O-glucoside (11) [36], biochanin-7-glucoside (12) [37,38], 9,10-dimethoxypterocarpan-3-O-xylosylglucoside (15) [39], 9,10-dimethoxypterocarpan-3-O-β-D-glucoside (16) [39], isomucronulatol-7-O-glucoside (17) [36,40], astragaloside VI (1′) [26,39,41], astragaloside VII (2′) [26,39,41], and astragaloside II (4′) [39,[41][42][43]. However, they still need to be further confirmed with the reference compounds and structural analysis by nuclear magnetic resonance (NMR) method.…”