Simultaneous determination of 11 antibiotics and their main metabolites from four different groups by reversed-phase high-performance liquid chromatography–diode array–fluorescence (HPLC–DAD–FLD) in human urine samples

Fernández‐Torres, Rut; Consentino, M. Olías; López, Miguel Ángel; Mochón, M. Callejón

doi:10.1016/j.talanta.2010.01.031

Cited by 70 publications

(32 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, after this time, AMXCs diastereomer were the preferably produced form for AMX‐related compounds at both pH values, suggesting that there are additional contributions involved during the environmental AMX transformation pathway. The absence of AMXC stereoisomers in all real samples (which also are the main metabolites of AMX35, 36) indicate its instability in environmental waters. An internal rearrangement that led to the formation of the diketopiperazine ring is the favored transformation while the ( 5R )‐AMX‐DKP stereoisomer was the only TP detected in surface waters.…”

Section: Resultsmentioning

confidence: 99%

Behavior of amoxicillin in wastewater and river water: identification of its main transformation products by liquid chromatography/electrospray quadrupole time‐of‐flight mass spectrometry

Pérez-Parada

Agüera

Gómez-Ramos

et al. 2011

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

The identification of transformation products (TPs) of pharmaceuticals in the environment is essentially a challenging task due to the lack of standards and the instrumental capabilities required to detect compounds (sometimes unknowns) that are produced under environmental conditions. In this work, we report the use of liquid chromatography/electrospray quadrupole time-of-flight mass spectrometry (LC/QTOF-MS/MS) as a tool for the identification of amoxicillin (AMX) and its main TPs in wastewater and river water samples. Laboratory degradation experiments of AMX were performed in both alkaline and acidic media in order to confirm that the expected transformation pathway in the aquatic media is through the β-lactam ring cleavage. A thorough study was carried out with both standards and real samples (wastewater and river water samples). Four compounds were identified as main TPs: both amoxicillin diketopiperacine-2',5' and amoxilloic acid diastereomers. Amoxilloic acid stereoisomers are reported for the first time in environmental matrices. The transformation product (5R)-amoxicillin diketopiperacine-2',5' was frequently detected in river waters. Besides, another AMX transformation product formed during analysis was also structurally elucidated for the first time (amoxicilloic acid methyl ester) via accurate mass measurements. Collected data show that although AMX is not present as such in environmental samples, different TPs occur. This study represent a valuable indicator of the potential of LC/QTOF-MS/MS for the identification and structural elucidation of TPs in the environment using accurate MS/MS experiments, enabling thus the recognition of the environmental transformation pathway.

show abstract

Section: Resultsmentioning

confidence: 99%

Behavior of amoxicillin in wastewater and river water: identification of its main transformation products by liquid chromatography/electrospray quadrupole time‐of‐flight mass spectrometry

Pérez-Parada

Agüera

Gómez-Ramos

et al. 2011

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

show abstract

“…TET was quantified using Agilent 1200 series HPLC equipped with a diode array and fluorescence detector (Fernandez-Torres et al, 2010;Liyanage & Manage, 2014). The injected volume was 20 μL and chromatography was performed at 30 0C.…”

Section: Hplc Quantificationmentioning

confidence: 99%

Occurrence and distribution of tetracycline resistance determinants and their pollution profile in the aquaculture environment in Sri Lanka

Liyanage

Manage

2019

J. Natn. Sci. Foundation Sri Lanka

View full text Add to dashboard Cite

Tetracycline (TET) has been extensively used in aquaculture for chemotherapy against various fish diseases. The contamination levels in 16 aquaculture farms in Sri Lanka were analysed using high performance liquid chromatography (HPLC). The presence of antibiotic-resistant bacteria was identified using the 16SrRNA gene sequencing and the corresponding antibiotic resistant genes (ARGs) were screened at each location using the PCR method. The concentration of TET in the aquaculture farm samples ranged between 0.001± 0.001 -0.112± 0.017 ppm. Bacillus and Staphylococcus were recorded as the most dominant resistant bacterial genera against TET. Acinetobacter sp., Achromabacter sp., Staphylococcus sp., Micrococcus sp. were also identified as resistant bacteria. In the present study, tet (M) and tet (A) were the abundant resistance genes (11/16 isolates), followed by tet (S) (5/16) and tet (B) (4/16). It was found that tet (M) is one of the most widely distributed tetracycline resistance determinants in the aquaculture environment in Sri Lanka.

show abstract

“…The analytical column Zorbax Eclipse XDB‐C18 (4.6 × 150 mm, id, 5 μm particle size) was used for antibiotic determination. The HPLC method for the separation of sulfamethoxazole and chloramphenicol used a gradient elution program with 0.7 mL/min flow rate. The mobile phase consisted of a mixture of 0.1% formic acid in water and acetonitrile.…”

Section: Methodsmentioning

confidence: 99%

Determination of Emerging Substances in the Danube and Potential Risk Evaluation

Letić

Milanović

Milić

et al. 2014

CLEAN Soil Air Water

View full text Add to dashboard Cite

Seasonal variations of caffeine and antibiotic concentrations were investigated along the selected sites of the Danube using solid-phase extraction followed by highperformance liquid chromatography (HPLC). The potential risks on the aquatic organisms were evaluated. HPLC methods for caffeine and antibiotic determination were modified and validated showing good accuracy, repeatability, selectivity, and robustness. The obtained results confirmed the presence of caffeine at four sampling points, near the wastewater discharges. The mean caffeine concentrations for summer, autumn, winter, and spring were 24.78, 26.83, 24.61, and 86.29 ng/L, respectively. The analyzed antibiotics (sulfamethoxazole, chloramphenicol, and tiamuline) were under the limit of detection in all tested samples taken from the Danube. The maximum risk indexes (MaxRIs) were calculated for caffeine at each sampling site. Four out of seven selected sampling sites showed sublethal effects on the aquatic organisms with 10 < MaxRI < 100 (class II), while the other three had no potential risk for aquatic organisms. The highest potential risk was calculated for the sampling site in the vicinity of one of the biggest wastewater discharges.

show abstract

Simultaneous determination of 11 antibiotics and their main metabolites from four different groups by reversed-phase high-performance liquid chromatography–diode array–fluorescence (HPLC–DAD–FLD) in human urine samples

Cited by 70 publications

References 47 publications

Behavior of amoxicillin in wastewater and river water: identification of its main transformation products by liquid chromatography/electrospray quadrupole time‐of‐flight mass spectrometry

Behavior of amoxicillin in wastewater and river water: identification of its main transformation products by liquid chromatography/electrospray quadrupole time‐of‐flight mass spectrometry

Occurrence and distribution of tetracycline resistance determinants and their pollution profile in the aquaculture environment in Sri Lanka

Determination of Emerging Substances in the Danube and Potential Risk Evaluation

Contact Info

Product

Resources

About