Simultaneous determination for oxicam non-steroidal anti-inflammatory drugs in human serum by liquid chromatography–tandem mass spectrometry

Shirako, Junichi; Kawasaki, Marina; Komine, Kotoe; Kunisue, Yoko; Terada, Masaru; Sasaki, Chizuko; Irie, Wataru; Murakami, Chikako; Tonooka, Keiko; Tomobe, Koji; Shinozuka, Tatsuo

doi:10.1016/j.forsciint.2012.11.016

Cited by 18 publications

(12 citation statements)

References 4 publications

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“…Although some of the chromatographic methods [14–20, 22, 23] seemed to have lower LODs and shorter analysis times, CZE methods need small amount of analyte volumes, and it takes shorter time to condition the capillary. This study has lower sensitivity than a previous chromatographic one [21].…”

Section: Resultsmentioning

confidence: 99%

Validated Method for the Determination of Piroxicam by Capillary Zone Electrophoresis and Its Application to Tablets

Dal

Oktayer

Doğrukol-Ak

2014

Journal of Analytical Methods in Chemistry

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Simple and rapid capillary zone electrophoretic method was developed and validated in this study for the determination of piroxicam in tablets. The separation of piroxicam was conducted in a fused-silica capillary by using 10 mM borate buffer (pH 9.0) containing 10% (v/v) methanol as background electrolyte. The optimum conditions determined were 25 kV for separation voltage and 1 s for injection time. Analysis was carried out with UV detection at 204 nm. Naproxen sodium was used as an internal standard. The method was linear over the range of 0.23–28.79 µg/mL. The accuracy and precision were found to be satisfied within the acceptable limits (<2%). The LOD and LOQ were found to be 0.07 and 0.19 µg/mL, respectively. The method described here was applied to tablet dosage forms and the content of a tablet was found in the limits of USP-24 suggestions. To compare the results of capillary electrophoretic method, UV spectrophotometric method was developed and the difference between two methods was found to be insignificant. The capillary zone electrophoretic method developed in this study is rapid, simple, and suitable for routine analysis of piroxicam in pharmaceutical tablets.

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Section: Resultsmentioning

confidence: 99%

Validated Method for the Determination of Piroxicam by Capillary Zone Electrophoresis and Its Application to Tablets

Dal

Oktayer

Doğrukol-Ak

2014

Journal of Analytical Methods in Chemistry

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“…Numerous analytical methods have been reported for analysis of LNC alone or in combination with other pharmacotherapeutic agent(s) in biological fluid, some of which include UV‐VIS, HPLC, HPLC‐MS/MS, LC‐MS, LC‐MS‐MS, LC‐ESI‐MS/MS, LC–MS/TOF, and LC–MS . Very few extraction methods have been employed for the analysis of LNC from plasma or blood.…”

Section: Introductionmentioning

confidence: 99%

“…Article Related Abbreviations: AUC, area under the curve; CPCSEA, committee for the purpose of control and supervision of experiments on animals; CV, coefficient of variance; IAEC, institutional animal ethical committee; IS, nternal standard; LLOQ, lower limit of quantification; LNC, Lornoxicam; MLX, Meloxicam; MRT, mean residence time; QC, quality control; TDM, therapeutic monitoring. Numerous analytical methods have been reported for analysis of LNC alone or in combination with other pharmacotherapeutic agent(s) in biological fluid, some of which include UV-VIS, HPLC, HPLC-MS/MS, LC-MS, LC-MS-MS, LC-ESI-MS/MS, LC-MS/TOF, and LC-MS [1,[4][5][6][7][8][9][10][11][12][13][14][15][16]. Very few extraction methods have been employed for the analysis of LNC from plasma or blood.…”

Section: Introductionmentioning

confidence: 99%

Lornoxicam quantification in rabbit plasma by reverse phase HPLC: Optimization and application to pharmacokinetic study

Salunkhe¹,

Jadhav

Bhinge³

2019

Separation Science Plus

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The aim of present study was to develop a simple, rapid, selective, sensitive and robust reverse phase high‐performance liquid chromatography method for quantification of Lornoxicam and internal standard meloxicam in rabbit plasma at the wavelength of 370 nm. Protein was precipitated from rabbit plasma sample by addition of acetonitrile as a vehicle. An isocratic elution of samples was performed on capcell pak C8 column with the mobile phase consisting 5 mM phosphate buffer (pH 4.8): methanol (40:60 v/v) delivered at flow rate 1.0 mL min−1. The method was quantitatively evaluated in terms of System suitability, linearity, precision, recovery, selectivity, robustness and stability studies. The validated reverse phase high‐performance liquid chromatography method was successfully applied for the bioavailability studies of Lornoxicam. The pharmacokinetic parameters were calculated for all the investigated drugs in rabbit plasma after single‐dose administrations of pure drug and formulation of Lornoxicam. Thus, developed method is simple, convenient and suitable for the analysis of Lornoxicam in bulk drug and pharmaceutical formulations.

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“…HPLC with UV/DAD (Bae, Kim, Jang, & Lee, ; Dasandi, Shivaprakash, Saroj, & Bhat, ; Liew, Loh, Tan, & Peh, ) had a sensitivity of about 50 ng/mL, but these methods needed a long chromatography run time (>12 min) and a large volume biological sample (>0.25 mL). High‐performance liquid chromatography–tandem mass spectrometry (HPLC‐MS/MS) was used in the determination of meloxicam (Ji, Lee, Kim, Jeong, & Lee, ; Lee, Ji, Kim, Lee, & Lee, ; Shirako et al, ). However its application was limited by the arduous and expensive sample preparation by solid‐phase extraction (Shirako et al, ) or time‐consuming and complicated sample preparation by liquid–liquid extraction (Ji et al, ; Lee et al, ), which could not meet the requirement for high throughput and speed in biosample analysis.…”

Section: Introductionmentioning

confidence: 99%

“…High‐performance liquid chromatography–tandem mass spectrometry (HPLC‐MS/MS) was used in the determination of meloxicam (Ji, Lee, Kim, Jeong, & Lee, ; Lee, Ji, Kim, Lee, & Lee, ; Shirako et al, ). However its application was limited by the arduous and expensive sample preparation by solid‐phase extraction (Shirako et al, ) or time‐consuming and complicated sample preparation by liquid–liquid extraction (Ji et al, ; Lee et al, ), which could not meet the requirement for high throughput and speed in biosample analysis. Yuan, Chen, and Zhong () reported an HPLC‐MS/MS method using rapid and simple sample preparation with protein precipitation (PPT) that had good sensitivity with a lower limit of quantification (LLOQ) of 0.10 ng/mL.…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of meloxicam in dog plasma by high performance liquid chromatography–tandem mass spectrometry and its application in a pharmacokinetic study

Tian

Xiao

Zhang

et al. 2018

Biomedical Chromatography

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A rapid, selective and sensitive high‐performance liquid chromatography–tandem mass spectrometry (HPLC‐MS/MS) method was developed to determine meloxicam in beagle dog plasma. Sample pretreatment involved a one‐step protein precipitation with methanol of 0.1 mL plasma. Analysis was performed on a Venusil ASB‐C18 column with mobile phase consisting of methanol–water (containing 0.1% formic acid) (75:25, v/v). The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring mode via electrospray ionization source. Each plasma sample was chromatographed within 4.1 min. The linear calibration curves for meloxicam was obtained in the concentration range of 10.3–4.12 × 103 ng/mL (r ≥ 0.99). The intra‐ and inter‐day precisions (relative standard deviation) were ≤ 15%, and accuracy (relative error) was within ±7.3%. The method herein described was fully validated and successfully applied to the pharmacokinetic study of meloxicam tablets in beagle dog.

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Simultaneous determination for oxicam non-steroidal anti-inflammatory drugs in human serum by liquid chromatography–tandem mass spectrometry

Cited by 18 publications

References 4 publications

Validated Method for the Determination of Piroxicam by Capillary Zone Electrophoresis and Its Application to Tablets

Validated Method for the Determination of Piroxicam by Capillary Zone Electrophoresis and Its Application to Tablets

Lornoxicam quantification in rabbit plasma by reverse phase HPLC: Optimization and application to pharmacokinetic study

Quantitative determination of meloxicam in dog plasma by high performance liquid chromatography–tandem mass spectrometry and its application in a pharmacokinetic study

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