Simultaneous determination and pharmacokinetics of tetrandrine, fangchinoline, and cyclanoline in rat plasma by ultra-high performance liquid chromatography-mass spectrometry after oral administration of stephaniae tetrandrae radix extract

Wang, Zhibin; Ma, Yueming; Hua, Liu; Bi, Yujin; Wang, Meng; Kuang, Hai‐Xue

doi:10.4103/wjtcm.wjtcm_73_20

Cited by 9 publications

(7 citation statements)

References 20 publications

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“…The therapeutic efficacy of tetrandrine in silicosis treatment has been widely recognized, [27] and it has been approved in China for this purpose. [3] The antifibrotic, anti-inflammatory, and hematopoietic properties of BYHWD have been extensively documented.…”

Section: Discussionmentioning

confidence: 99%

“…Tetrandrine is a bisbenzylisoquinoline alkaloid that possesses anti-inflammatory and antifibrotic properties. [27,42] Recently, Song et al [4] demonstrated that tetrandrine attenuated silicosis by inhibiting the NLRP3 inflammatory receptor in macrophages by utilizing single-cell RNA sequencing and transcriptomics of lung tissue as well as quantitative microarray analysis of serum from silicosis and control mice. Furthermore, it was revealed that tetrandrine exhibited enrichment in the Toll-like receptor signaling pathway during the treatment of silicosis.…”

Section: Discussionmentioning

confidence: 99%

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Unraveling the mechanism of tetrandrine combined with Buyang Huanwu Decoction against silicosis using network pharmacology and molecular docking analyses

Zhao

et al. 2023

Medicine

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Silicosis is an incurable chronic disease characterized by lung fibrosis and inflammation. The combination of tetrandrine and Buyang Huanwu Decoction (BYHWD) has a curative effect on silicosis. However, the mechanism of action and the key active constituent in BYHWD are still unclear. The present study employed network pharmacology and molecular docking to determine the mechanism of action and the key active components of BYHWD of Tetrandrine in combination with BYHWD for silicosis. The primary elements and targets of BYHWD were obtained from the Traditional Chinese Medicine Systems Pharmacology and analysis platform. The targets associated with tetrandrine and silicosis were identified and extracted from the Comparative Toxicogenomics Database and GeneCards database. The potential targets for the treatment of silicosis using a combination of Tetrandrine and BYHWD were identified by considering the overlapping targets between compound drugs and silicosis. These targets were then utilized to construct protein-protein interaction networks, compound drug-ingredient-target networks, and perform enrichment analyses. The top 5 active ingredients present in the compound drug-ingredient-target network are tetrandrine, quercetin, luteolin, kaempferol, and beta-carotene. Similarly, the top 6 hub genes in the protein-protein interaction network are FGF2, MMP-9, MMP-1, IL-10, IL-17A, and IL-6. The molecular docking suggested that the active components may easily access the active pocket of the hub gene. The in-silico investigation suggested that quercetin might be the active component in BYHWD responsible for therapeutic effectiveness against silicosis. This study identified the active compound and potential molecular mechanism underlying the therapeutic effects of BYHWD in combination with tetrandrine for treating silicosis. Notably, we found that quercetin may serve as the key compound in BYHWD for the treatment of silicosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Unraveling the mechanism of tetrandrine combined with Buyang Huanwu Decoction against silicosis using network pharmacology and molecular docking analyses

Zhao

et al. 2023

Medicine

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“…Elsafadi et al reported that the differentiation of human bone marrow stromal (skeletal or mesenchymal) stem cells is denoted by a decrease in a-SMA (Elsafadi et al, 2019). Radiation injury predisposes to the formation of fibrosis in damaged tissues, and the fibrotic process is closely related to the EMT process, which refers to the process The Wnt/β-catenin (or classic Wnt) signaling pathway is highly conserved during tissue development and regulates the differentiation, proliferation, death, and function of various cells (He & Tang, 2020;Li, Lu, et al, 2022;Li, Zhang, et al, 2022;Wang, Ma, et al, 2021). It is important for the maintenance of multiple epithelial stem cells and is a key driver of most types of tissue stem cells in adult mammals (Nusse & Clevers, 2017).…”

Section: Discussionmentioning

confidence: 99%

Repair mechanism of radiation‐induced salivary gland injury by hypoxia‐pretreated human urine‐derived stem cell exosomes

et al. 2023

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ObjectiveTo explore the protective effect of human urine‐derived stem cell exosomes (hUSC‐Exos) on radiation‐induced salivary gland (SG) injuries in Sprague Dawley rats.MethodsFresh adult urine was collected, and primary hUSCs were isolated and identified. The hUSCs were hypoxia‐pretreated with 1% oxygen for 24 h and then transferred to a normoxic culture environment for 24 h. The hUSC‐Exos were collected and identified for exosomes. A radiation‐induced injury model was established in the rats, and exosomes were introduced by local injection in the SG and tail vein. The submandibular gland was excised for morphological observation 1 week later. Immunohistochemical detection of the glandular tissue was conducted by α‐smooth muscle actin (a‐SMA), stem cell growth factor receptor (c‐Kit) staining, and periodic acid–Schiff staining. Qualitative polymerase chain reaction and western blot analysis were adopted to detect the gene and protein expression of Wnt3a, GSK3β, and Axin.ResultsIn both the normoxic and hypoxic hUSC‐Exo groups, microvesicular structures with bilayer membranes of approximately 80 nm in diameter were detected, and the expressions of CD9 and CD63 were detected by nanoflow cytometry. Compared with the control group, in the radiation‐induced injury model group, the expression of a‐SMA was significantly higher, the expression of c‐Kit was significantly lower, and the expressions of Wnt3a, GSK3β, and Axin were significantly upregulated; the differences were statistically significant (p < 0.05). Compared with the model group, in the normoxic and hypoxic hUSC‐Exo groups, the expression of a‐SMA was significantly decreased, the expression of c‐Kit was significantly increased, and the expressions of Wnt3a, GSK3β, and Axin were significantly upregulated; the differences were statistically significant (p < 0.05).ConclusionHypoxia‐pretreated hUSC‐Exos could repair radiation‐induced SG injuries by activating the Wnt3a/GSK3β pathway to suppress the expressions of a‐SMA and c‐Kit.

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“…These factors are important for developing resilience and are frequently the focus of interventions aimed at mitigating the negative effects of trauma. According to statistics, the prevalence of dental caries in children aged 3–5 years in China is as high as 66.0%, and the incidence of dental trauma in children aged 12 years is as high as 19.5% [ 3 ], but the visit rate and caries supplementation ratio are only 19.4% and 3.1% [ 4 ]. Because the root of the tooth is located in the alveolar bone, in order to accurately understand the tooth arrangement and root distribution and collision characteristics, dentists need to evaluate the tooth status of patients in detail based on imaging techniques, cone beam computed tomography (CBCT) technology can diagnose tooth, periodontal, and jaw diseases due to its advantages of high imaging accuracy and low artifact rate [ 5 ]; and in addition, it provides a reference basis for the selection of treatment options and prognosis, so it is used as a common means of diagnosis and treatment of oral diseases.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of children’s oral diagnosis and treatment using imaging examination using AI based Internet of Things

Li,

Qu,

Yue

et al. 2024

THC

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BACKGROUND: Although cone beam computed tomography (CBCT) plays an important role in the diagnosis and treatment of oral diseases, its image segmentation method needs to be further improved, and there are still objections about the clinical application effect of general anesthesia (GA) on children’s dental fear (CDF). OBJECTIVE: This study aimed to investigate the application value of CBCT based on intelligent computer segmentation model in oral diagnosis and treatment of children in the context of biomedical signals, and to analyze the alleviating effect of GA on CDF. METHODS: Based on the regional level set (CV) algorithm, the local binary fitting (LBF) model was introduced to optimize it, and the tooth CBCT image segmentation model CV-LBF was established to compare the segmentation accuracy (SA), maximum symmetric surface distance (MSSD), average symmetric surface distance (ASSD), over segmentation rate (OR), and under segmentation rate (UR) between these model and other algorithms. 82 children with CDF were divided into general anesthesia group (GAG) (n= 38) and controls (n= 44) according to the voluntary principle of their families. Children in GAG were treated with GA and controls with protective fixed intervention. Children’s fear survey schedule-dental subscale (CFSS-DS) and Venham scores were counted before intervention in the two groups. CFSS-DS scores were recorded at 2 hours after intervention and after recovery in children in GAG. CFSS-DS and Venham scores were performed in all children 1 week after surgery. RESULTS: The results showed that the S⁢A value of CV-LBF algorithm was higher than that of region growing algorithm (P< 0.05). OR, UR, MSSD, and ASSD values of CV-LBF algorithm were evidently lower than those of other algorithms (P< 0.05). CFSS-DS scores were lower in GAG than in controls 2 hours after intervention and at return visits after 1 week of intervention (P< 0.001), and Venham scores were lower in GAG than in controls after intervention (P< 0.001). After intervention, the proportion of children with Venham grade 0, 1, 2, and 3 was obviously higher in GAG than in controls (P< 0.001), while the proportion of children with Venham grade 4 and 5 was clearly higher in controls than in GAG (P< 0.001). CONCLUSION: The results revealed that the computer intelligent segmentation model CV-LBF has potential application value in CBCT image segmentation of children’s teeth, and GA can effectively alleviate anxiety of children with CDF and can be used as biomedical signals.

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Simultaneous determination and pharmacokinetics of tetrandrine, fangchinoline, and cyclanoline in rat plasma by ultra-high performance liquid chromatography-mass spectrometry after oral administration of stephaniae tetrandrae radix extract

Cited by 9 publications

References 20 publications

Unraveling the mechanism of tetrandrine combined with Buyang Huanwu Decoction against silicosis using network pharmacology and molecular docking analyses

Unraveling the mechanism of tetrandrine combined with Buyang Huanwu Decoction against silicosis using network pharmacology and molecular docking analyses

Repair mechanism of radiation‐induced salivary gland injury by hypoxia‐pretreated human urine‐derived stem cell exosomes

Evaluation of children’s oral diagnosis and treatment using imaging examination using AI based Internet of Things

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